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Combined in vitro effects of temozolomide and erucic acid on U87 MG cell viability, oxidative stress, and expression of genes involved in apoptotic signalling Cover

Combined in vitro effects of temozolomide and erucic acid on U87 MG cell viability, oxidative stress, and expression of genes involved in apoptotic signalling

Archives of Industrial Hygiene and Toxicology
Volume 77 (2026): Issue 2 (June 2026)
By: ,  ,  ,  ,  ,  ,  ,   and    
Open Access
|Jun 2026
Figures & tables

Figures & Tables

Figure 1

Cell viability and LDH release following the treatment of U87 MG cells with TMZ (200 ng/mL) alone or in combination with EA (20, 40, 80, and 160 µg/mL) for 24 h and 72 h. The data are presented as means ± SD of 8 independent biological replicates. Within each replicate, five technical wells were averaged to obtain one value. a P=0.028; b P=0.019; c P=0.006; d P=0.004; e P=0.002; f P=0.0009; g P=0.029; h P=0.018; i P=0.004; j P=0.006; k P=0.0008 compared to control (one-way ANOVA followed by Dunnett’s test). EA – erucic acid; TMZ – temozolomide

Figure 2

Representative phase-contrast images showing morphological changes in U87 MG cells following treatment with TMZ (200 ng/mL) alone or in combination with EA (20, 40, 80, and 160 µg/mL) for 72 h. Images were acquired using a Nikon ELWD 20× objective (N.A. 0.3) with a 10× ocular lens, yielding a total magnification of 200×. EA – erucic acid; TMZ – temozolomide

Figure 3

TAC and TOS levels following the treatment of U87 MG cells with TMZ (200 ng/mL) alone or in combination with EA (20, 40, 80, and 160 µg/mL) for 24 h and 72 h. The data are presented as means ± SD of 8 independent biological replicates. Within each replicate, five technical measurements were averaged to obtain one value. a P=0.041; b P=0.014; c P=0.009; d P=0.004; e P=0.047; f P=0.028; g P=0.009; h P=0.006; i P=0.006; j P=0.004; k P=0.002; l P=0.0007; m P=0.008; n P=0.006; o P=0.002; p P=0.0009 compared to control (one-way ANOVA followed by Dunnett’s test). EA – erucic acid; TAC – total antioxidant capacity; TMZ – temozolomide; TOS – total oxidant status

Figure 4

Relative mRNA expression of Bax, Bcl-2, Bcl-xL, caspase-3, p53, and p21 following the treatment of U87 MG cells with TMZ (200 ng/mL) alone or in combination with EA (20, 40, 80, and 160 µg/mL) for 24 h and 72 h. The data are presented as means ± SD of 3 independent biological replicates. Within each replicate, five technical measurements were averaged to obtain one value. aP=0.032; bP=0.028; cP=0.019; dP=0.008; eP=0.008; fP=0.004; gP=0.009; hP=0.006; iP=0.032; jP=0.006; kP=0.002; lP=0.032; mP=0.006; nP=0.002; oP=0.028; pP=0.019; qP=0.004 compared to control (one-way ANOVA followed by Dunnett’s test). EA – erucic acid; TMZ – temozolomide
DOI: https://doi.org/10.2478/aiht-2026-77-4098 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English
Page range: 123 - 131
Submitted on: Jan 1, 2026
Accepted on: May 1, 2026
Published on: Jun 30, 2026
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Keywords:
cancer therapy,
chemotherapy,
cytotoxicity,
fatty acids,
glioblastoma,
oxidative stress
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2026 İbrahim Gecili, Sıdıka Genç, Ahmet Hacımüftüoğlu, Adem Güner, Esmanur Niğde, Kübra Karabulut, Dündar Okan Yıllar, Ali Taghizadehehghalehjoughi, Abdelaty Mostafa Abdelaty Hassibelnaby, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 77 (2026): Issue 2 (June 2026)
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