Figure 1
Effect of different extraction conditions on the peak area. Citrate buffer (0.1 mol/L, pH=4, V=10 mL) was used for extraction. Samples [dehydrated lucerne (sample 1) with a mass fraction of 327 ng/g codeine and 1510 ng/g morphine] were prepared in triplicate. Results were expressed as mean ± standard deviation
Figure 2
Total and selected ion chromatograms of a lucerne sample spiked with a standard to contain 400 ng/g of morphine and codeine. TMS–trimethylsilyl; 6-MAM–6-monoacetylmorphine, internal standard
Table 1
Limit of detection and quantification, accuracy, and precision for determining the mass fraction of morphine and codeine in dehydrated lucerne samples (n=6)
| Analyte | ω (ng/g) | Precision (RSD %) | Accuracy (%) | Limit of detection (ng/g) | Limit of quantification (ng/g) |
|---|---|---|---|---|---|
| Morphine | 100 | 2.0 | 95.5 | 22 | 73 |
| 800 | 0.6 | 98.9 | |||
| Codeine | 100 | 3.9 | 96.2 | 25 | 83 |
| 800 | 1.1 | 99.7 |
Figure 3
Total and selected ion chromatograms of dehydrated lucerne (sample 1) with a mass fraction of 327 ng/g codeine and 1510 ng/g morphine. TMS–trimethylsilyl; 6-MAM–6-monoacetylmorphine, internal standard