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Urine levels of nicotine and its metabolites in young population exposed to second-hand smoke in nightclubs: a pilot study Cover

Urine levels of nicotine and its metabolites in young population exposed to second-hand smoke in nightclubs: a pilot study

Open Access
|Sep 2024

Figures & Tables

Figure 1

Study design
Study design

Figure 2

Differences in median nicotine (red column), cotinine (grey column), and 3HC (green column) increase (in ng/L) in urine between non-smokers (NS) and smokers (S)(chi-squared test). Box plots show the median, interquartile range (box), and overall range (whiskers). p<0.05 – statistically significant
Differences in median nicotine (red column), cotinine (grey column), and 3HC (green column) increase (in ng/L) in urine between non-smokers (NS) and smokers (S)(chi-squared test). Box plots show the median, interquartile range (box), and overall range (whiskers). p<0.05 – statistically significant

Figure 3

Differences in the ratio between median baseline and post-exposure nicotine (red column), cotinine (grey column), and 3HC (green column) concentrations in urine between non-smokers (NS) and smokers (S) (chi-squared test). The y-axis shows how many times urine concentration increased between baseline and the post-exposure measurement. p<0.05 – statistically significant
Differences in the ratio between median baseline and post-exposure nicotine (red column), cotinine (grey column), and 3HC (green column) concentrations in urine between non-smokers (NS) and smokers (S) (chi-squared test). The y-axis shows how many times urine concentration increased between baseline and the post-exposure measurement. p<0.05 – statistically significant

Nicotine, cotinine, and trans-3′-hydroxycotinine concentrations in urine samples of 22 study participants before (baseline) and after having spent 190 min in a nightclub and slept for six hours in average (post-exposure)

VariableBaseline concentrations (ng/mL)Post-exposure concentrations (ng/mL)Differences between baseline and post-exposure measurements*
MinMaxMedian (IQR)MinMaxMedian (IQR)
Smokers (N=5)Nicotine0.66323.3416.22 (172.21)25.20509.58364.72 (370.88)p<0.05
Cotinine2.61323.1554.44 (235.22)54.82946.04373.06 (639.29)p=0.095
3HC5.46451.51168.41 (328.16)<LOD5933.42575.15 (3681.79)p=0.529
Non-smokers (N=17)Nicotine0.6742.252.66 (14.26)6.91588.2732.37 (49.29)p<0.05
Cotinine<LOD63.0035.36 (40.49)2.62190.5565.09 (66.94)p<0.05
3HC1.2377.765.46 (16.52)2.46148.7732.95 (68.96)p<0.05
DOI: https://doi.org/10.2478/aiht-2024-75-3846 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
Language: English, Croatian, Slovenian
Page range: 211 - 216
Submitted on: Mar 1, 2024
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Accepted on: Aug 1, 2024
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Published on: Sep 29, 2024
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2024 Antonia Zečić, Bernarda Vazdar, Livia Slišković, Davorka Sutlović, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.