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Nanoplastics increase in vitro oestrogenic activity of neurotherapeutic drugs Cover

Nanoplastics increase in vitro oestrogenic activity of neurotherapeutic drugs

Archives of Industrial Hygiene and Toxicology
Volume 75 (2024): Issue 1 (March 2024)
By: Lucija Božičević,  Valerije Vrček,  Nikolina Peranić,  Nikolina Kalčec and  Ivana Vinković Vrček  
Open Access
|Mar 2024

Figures & Tables

Figure 1

Transmission electron micrograph (TEM) and physico-chemical properties of 10 mg/L polystyrene nanoparticles (PSNPs) in ultrapure water (UPW) and the RPMI-1640 cell culture medium (CCM) at baseline (t=0 h) and after 48 h (t=48 h) at 25 °C. dTEM – PSNP dispersion in ultrapure-water, dH – PSNP hydrodynamic diameter in UPW and CCM; ζ – PSNP zeta potential in in UPW and CCM
Transmission electron micrograph (TEM) and physico-chemical properties of 10 mg/L polystyrene nanoparticles (PSNPs) in ultrapure water (UPW) and the RPMI-1640 cell culture medium (CCM) at baseline (t=0 h) and after 48 h (t=48 h) at 25 °C. dTEM – PSNP dispersion in ultrapure-water, dH – PSNP hydrodynamic diameter in UPW and CCM; ζ – PSNP zeta potential in in UPW and CCM

Figure 2

Viability of T47D-KBluc cells treated with A) carbamazepine (Cbz) and B) fluoxetine (Flx) established with the MTS assay. Untreated cells were used as negative control (Ctrl) and cells treated with 10 % (v/v) DMSO as positive control (DMSO). Results are given as the percentage of Ctrl and calculated as mean values from three independent experiments. Standard deviations are given as error bars, and values that are significantly different from Ctrl are marked with * (P<0.05)
Viability of T47D-KBluc cells treated with A) carbamazepine (Cbz) and B) fluoxetine (Flx) established with the MTS assay. Untreated cells were used as negative control (Ctrl) and cells treated with 10 % (v/v) DMSO as positive control (DMSO). Results are given as the percentage of Ctrl and calculated as mean values from three independent experiments. Standard deviations are given as error bars, and values that are significantly different from Ctrl are marked with * (P<0.05)

Figure 3

Viability and apoptosis in T47D-KBluc cells treated with PSNPs, Cbz and Flx established with flow cytometry. Untreated cells were used as negative control (Ctrl) and cells treated with 10 % (v/v) DMSO as positive control (DMSO). Results are given as the percentage of live, early apoptotic, late apoptotic, and dead cells compared to Ctrl and calculated as mean values from three independent experiments. Standard deviations are given as error bars
Viability and apoptosis in T47D-KBluc cells treated with PSNPs, Cbz and Flx established with flow cytometry. Untreated cells were used as negative control (Ctrl) and cells treated with 10 % (v/v) DMSO as positive control (DMSO). Results are given as the percentage of live, early apoptotic, late apoptotic, and dead cells compared to Ctrl and calculated as mean values from three independent experiments. Standard deviations are given as error bars

Figure 4

Oestrogen receptor activity in response to carbamazepine (Cbz), fluoxetine (Flx), and polystyrene nanoparticle (PSNP) treatment of T47D-KBluc cells. Results are shown as the percentage of fold induction of luminescent signal in comparison with positive control (10 nmol/L diethylstilbestrol, DES) and represent mean values from three independent experiments done in triplicate. Standard deviations (SD) are given as error bars. Values above the black line denote endocrine disrupting response according to the OECD test No. 455 (5)
Oestrogen receptor activity in response to carbamazepine (Cbz), fluoxetine (Flx), and polystyrene nanoparticle (PSNP) treatment of T47D-KBluc cells. Results are shown as the percentage of fold induction of luminescent signal in comparison with positive control (10 nmol/L diethylstilbestrol, DES) and represent mean values from three independent experiments done in triplicate. Standard deviations (SD) are given as error bars. Values above the black line denote endocrine disrupting response according to the OECD test No. 455 (5)

Figure 5

Oestrogen receptor activity in response to PSNP mixtures with (A) carbamazepine (Cbz) and (B) fluoxetine (Flx) in T47D-KBluc cells. Results are shown as the percentage of fold induction of luminescent signal in comparison with positive control (10 nmol/L diethylstilbestrol, DES) and represent mean values from three independent experiments done in triplicate. Standard deviations (SD) are given as error bars. Values above the black line denote endocrine disrupting response according to the OECD test No. 455 (5)
Oestrogen receptor activity in response to PSNP mixtures with (A) carbamazepine (Cbz) and (B) fluoxetine (Flx) in T47D-KBluc cells. Results are shown as the percentage of fold induction of luminescent signal in comparison with positive control (10 nmol/L diethylstilbestrol, DES) and represent mean values from three independent experiments done in triplicate. Standard deviations (SD) are given as error bars. Values above the black line denote endocrine disrupting response according to the OECD test No. 455 (5)
DOI: https://doi.org/10.2478/aiht-2024-75-3818 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 68 - 75
Submitted on: Jan 1, 2024
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Accepted on: Mar 1, 2024
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Published on: Mar 29, 2024
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
polystyrene,
carbamazepine,
fluoxetine,
oestrogen receptors,
T47D-KBluc cell line
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2024 Lucija Božičević, Valerije Vrček, Nikolina Peranić, Nikolina Kalčec, Ivana Vinković Vrček, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution 4.0 License.

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