Figure 1
Experimental design. s.c. – subcutaneous; i.p. – intraperitoneal; p.o. – per os; TC – tumour control; IRI – irinotecan; THC – Δ9-tetrahydrocannabinol
Figure 2
Changes in mouse body weight following treatment with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Values represent the mean of five mice per group.
Table 1
Relative brain and liver weights in syngeneic colorectal cancer mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control - TC)
| Relative weight (%) | ||||
|---|---|---|---|---|
| TC | IRI | THC | IRI + THC | |
| Brain | 1.42±0.05 | 1.48±0.12 | 1.36±0.04 | 1.35±0.09 |
| Liver | 5.37±0.11 | 5.36±0.21 | 5.24±0.09 | 5.66±0.24 |
Figure 3
Changes in average tumour volume in syngeneic colorectal mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Values are expressed as means ± SEM of five mice per group. a significantly different vs IRI (p<0.05).
Figure 4
Changes in total cholinesterase (ChE), acetylcholinesterase (AChE), and butyrylcholinesterase activities (BChE) in the whole blood of syngeneic colorectal cancer mice after treatment with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Results are presented as medians and interquartile ranges. * significantly different from control (Kruskal-Wallis test, p<0.05)
Figure 5
Changes in oxidative stress parameters in the plasma and erythrocytes of syngeneic colorectal cancer mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control, TC). (a) The levels of reactive oxygen species (ROS) in the plasma; (b) the levels of glutathione (GSH) in the plasma; (c) concentrations of thiobarbituric reactive substances (TBARS) in the plasma; (d) erythrocyte catalase (CAT) activity; (e) erythrocyte superoxide dismutase (SOD) activity; (f) erythrocyte glutathione peroxidase (GPx) activity. * significantly higher than TC (p<0.05); a significantly higher than IRI (p<0.05); b significantly higher than THC (p<0.05)