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Effects of concomitant use of THC and irinotecan on tumour growth and biochemical markers in a syngeneic mouse model of colon cancer Cover

Effects of concomitant use of THC and irinotecan on tumour growth and biochemical markers in a syngeneic mouse model of colon cancer

Archives of Industrial Hygiene and Toxicology
Volume 74 (2023): Issue 3 (September 2023)
By: Suzana Žunec,  Irena Brčić Karačonji,  Martin Čatalinac,  Andreja Jurič,  Anja Katić,  Goran Kozina,  Vedran Micek,  Marijana Neuberg and  Ana Lucić Vrdoljak  
Open Access
|Sep 2023

Figures & Tables

Figure 1

Experimental design. s.c. – subcutaneous; i.p. – intraperitoneal; p.o. – per os; TC – tumour control; IRI – irinotecan; THC – Δ9-tetrahydrocannabinol
Experimental design. s.c. – subcutaneous; i.p. – intraperitoneal; p.o. – per os; TC – tumour control; IRI – irinotecan; THC – Δ9-tetrahydrocannabinol

Figure 2

Changes in mouse body weight following treatment with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Values represent the mean of five mice per group.
Changes in mouse body weight following treatment with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Values represent the mean of five mice per group.

Figure 3

Changes in average tumour volume in syngeneic colorectal mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Values are expressed as means ± SEM of five mice per group. a significantly different vs IRI (p<0.05).
Changes in average tumour volume in syngeneic colorectal mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Values are expressed as means ± SEM of five mice per group. a significantly different vs IRI (p<0.05).

Figure 4

Changes in total cholinesterase (ChE), acetylcholinesterase (AChE), and butyrylcholinesterase activities (BChE) in the whole blood of syngeneic colorectal cancer mice after treatment with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Results are presented as medians and interquartile ranges. * significantly different from control (Kruskal-Wallis test, p<0.05)
Changes in total cholinesterase (ChE), acetylcholinesterase (AChE), and butyrylcholinesterase activities (BChE) in the whole blood of syngeneic colorectal cancer mice after treatment with IRI, THC, their combination (IRI+THC), and in respective control (tumour control – TC). Results are presented as medians and interquartile ranges. * significantly different from control (Kruskal-Wallis test, p<0.05)

Figure 5

Changes in oxidative stress parameters in the plasma and erythrocytes of syngeneic colorectal cancer mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control, TC). (a) The levels of reactive oxygen species (ROS) in the plasma; (b) the levels of glutathione (GSH) in the plasma; (c) concentrations of thiobarbituric reactive substances (TBARS) in the plasma; (d) erythrocyte catalase (CAT) activity; (e) erythrocyte superoxide dismutase (SOD) activity; (f) erythrocyte glutathione peroxidase (GPx) activity. * significantly higher than TC (p<0.05); a significantly higher than IRI (p<0.05); b significantly higher than THC (p<0.05)
Changes in oxidative stress parameters in the plasma and erythrocytes of syngeneic colorectal cancer mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control, TC). (a) The levels of reactive oxygen species (ROS) in the plasma; (b) the levels of glutathione (GSH) in the plasma; (c) concentrations of thiobarbituric reactive substances (TBARS) in the plasma; (d) erythrocyte catalase (CAT) activity; (e) erythrocyte superoxide dismutase (SOD) activity; (f) erythrocyte glutathione peroxidase (GPx) activity. * significantly higher than TC (p<0.05); a significantly higher than IRI (p<0.05); b significantly higher than THC (p<0.05)

Relative brain and liver weights in syngeneic colorectal cancer mice treated with IRI, THC, their combination (IRI+THC), and in respective control (tumour control - TC)

Relative weight (%)
TCIRITHCIRI + THC
Brain1.42±0.051.48±0.121.36±0.041.35±0.09
Liver5.37±0.115.36±0.215.24±0.095.66±0.24
DOI: https://doi.org/10.2478/aiht-2023-74-3765 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 198 - 206
Submitted on: Jul 1, 2023
Accepted on: Sep 1, 2023
Published on: Sep 30, 2023
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
antitumour activity,
cannabinoid-based preparations,
oxidative stress,
systemic toxicity
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2023 Suzana Žunec, Irena Brčić Karačonji, Martin Čatalinac, Andreja Jurič, Anja Katić, Goran Kozina, Vedran Micek, Marijana Neuberg, Ana Lucić Vrdoljak, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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