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New in vitro findings about halogenated boroxine cytotoxicity and deregulation of cell death-related genes in GR-M melanoma cells Cover

New in vitro findings about halogenated boroxine cytotoxicity and deregulation of cell death-related genes in GR-M melanoma cells

Archives of Industrial Hygiene and Toxicology
Volume 74 (2023): Issue 1 (March 2023)
By: Nikolina Elez-Burnjaković,  Lejla Pojskić,  Anja Haverić,  Naida Lojo-Kadrić,  Maida Hadžić Omanović,  Jasmin Ramić,  Ajla Smajlović,  Milka Maksimović and  Sanin Haverić  
Open Access
|Apr 2023

Figures & Tables

Figure 1

Growth inhibition of GR-M and PBM cells treated with different concentrations of halogenated boroxine; *p<0.05
Growth inhibition of GR-M and PBM cells treated with different concentrations of halogenated boroxine; *p<0.05

Primer sequences used for selected cell death-related genes

GeneReference sequence No. (FASTA format)Primers
BECN1NM_003766.5Forward: CTCCCGAGGTGAAGAGCATCReverse: GGGGGATGAATCTGCGAGAG
SQSTM1NM_003900.5Forward: CCGTGAAGGCCTACCTTCTGReverse: TCCTCGTCACTGGAAAAGGC
BCL-2NM_000633.3Forward: GGGGTCATGTGTGTGGAGAGReverse: GAAATCAAACAGAGGCCGCA
DRAM1NM_018370.3Forward: TTGGTGCAGCCACGATGTATReverse: ACACCACAGACAAAGGCCAA

Changes in relative BCL-2 gene expression after GR-M and PBM cell treatment with halogenated boroxine compared to negative control

HB concentrations (mg/mL)GR-M cellsPBM cells

Fold change/P value

Fold change/P value
0.05down-3.805/0.672up2.04/0.001
0.1up11.769/0.001down-1.158/0.677
0.2up1.568/0.001up2.14/0.001
0.4down-10.933/0.001down-1.262/0.169
0.8down-1.041/0.848up6.365/0.001

Changes in relative DRAM1 gene expression after GR-M and PBM cell treatment with halogenated boroxine compared to negative control

HB concentrations (mg/mL)GR-M cellsPBM cells

Fold change/P value

Fold change/P value
0.05down1.661/0.3405up15.778/0.667
0.1down1.098/0.671up459.792/0.001
0.2down1.209/0.828up7968.193/0.001
0.4down4.195/0.001up6014.928/0.001
0.8down2.344/0.001//

Changes in relative BECN1 gene expression after GR-M and PBM cell treatment with halogenated boroxine compared to negative control

HB concentrations (mg/mL)GR-M cellsPBM cells

Fold change/P value

Fold change/P value
0.05up1.788/0.001up3.884/0.001
0.1down2.143/0.0815up1.848/0.001
0.2up2.202/0.322up15.735/0.001
0.4up1.04/0.83up62.542/0.001
0.8down3.188/0.001up72636.933/0.001

Changes in relative SQSTM1 gene expression after GR-M and PBM cell treatment with halogenated boroxine compared to negative control

HB concentrations (mg/mL)GR-M cellsPBM cells

Fold change/P value

Fold change/P value
0.05up1.387/0.1695down1.078/0.6465
0.1up3.643/0.001down1.951/0.153
0.2up11.612/0.001up5.764/0.001
0.4up6.92/0.001up7.424/0.001
0.8up3.295/0.001up1.004/0.6465
DOI: https://doi.org/10.2478/aiht-2023-74-3702 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 16 - 21
Submitted on: Dec 1, 2022
|
Accepted on: Feb 1, 2023
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Published on: Apr 4, 2023
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
anti-proliferative effect,
BCL-2,
BECN1, DRAM1,
human Caucasian melanoma,
peripheral blood mononuclear cell,
SQSTM1
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2023 Nikolina Elez-Burnjaković, Lejla Pojskić, Anja Haverić, Naida Lojo-Kadrić, Maida Hadžić Omanović, Jasmin Ramić, Ajla Smajlović, Milka Maksimović, Sanin Haverić, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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