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How different microfilters affect the recovery of eleven EU-regulated mycotoxins Cover

How different microfilters affect the recovery of eleven EU-regulated mycotoxins

Archives of Industrial Hygiene and Toxicology
Volume 74 (2023): Issue 1 (March 2023)
By: Marija Kovač Tomas,  Anto Mijatović,  Mateja Bulaić Nevistić,  Bojan Šarkanj,  Jurislav Babić and  Tihomir Kovač  
Open Access
|Apr 2023

Figures & Tables

Figure 1

Chemical structures of EU-regulated mycotoxins (excluding ergot alkaloids)
Chemical structures of EU-regulated mycotoxins (excluding ergot alkaloids)

Figure 2

Microfiltrate preparation. Filtrate solutions (vials) 1 and 2 were injected into the UHPLC column one at a time
Microfiltrate preparation. Filtrate solutions (vials) 1 and 2 were injected into the UHPLC column one at a time

Figure 3

UHPLC-MS/MS chromatogram of the selected EU-regulated mycotoxins in the unfiltered multi-mycotoxin standard solution (AFB1/AFG1 0.5 ng/mL, AFB2/AFG2 0.125 ng/mL, DON 50 ng/mL, FB1/FB2 37.5 ng/mL, ZEA 7.5 ng/mL, T-2/HT-2 2.5 ng/mL and OTA 0.25 ng/mL) obtained with an Acquity H-class – Xevo TQ-S micro system
UHPLC-MS/MS chromatogram of the selected EU-regulated mycotoxins in the unfiltered multi-mycotoxin standard solution (AFB1/AFG1 0.5 ng/mL, AFB2/AFG2 0.125 ng/mL, DON 50 ng/mL, FB1/FB2 37.5 ng/mL, ZEA 7.5 ng/mL, T-2/HT-2 2.5 ng/mL and OTA 0.25 ng/mL) obtained with an Acquity H-class – Xevo TQ-S micro system

Figure 4

Recoveries of mycotoxins with various filter membrane materials in filtrate solution 1 and 2 relative to the recovery (dashed line) from the unfiltered multi-mycotoxin solution. Low recoveries indicating mycotoxin loss during filtration are framed in squares. CA – cellulose acetate; MCE – mixed cellulose ester; NY – nylon; PES – polyethersulphone; PTFE – polytetrafluoroethylene
Recoveries of mycotoxins with various filter membrane materials in filtrate solution 1 and 2 relative to the recovery (dashed line) from the unfiltered multi-mycotoxin solution. Low recoveries indicating mycotoxin loss during filtration are framed in squares. CA – cellulose acetate; MCE – mixed cellulose ester; NY – nylon; PES – polyethersulphone; PTFE – polytetrafluoroethylene

Figure 5

Relative recovery of the 11 mycotoxins in filtrate solutions 1 and 2 by filter membrane material. CA – cellulose acetate; FS1 – filtrate solution 1 (first three drops of the solution); FS2 – filtrate solution 2 (the rest of the solution); MCE – mixed cellulose ester; NY – nylon; PES – polyethersulphone; PTFE – polytetrafluoroethylene
Relative recovery of the 11 mycotoxins in filtrate solutions 1 and 2 by filter membrane material. CA – cellulose acetate; FS1 – filtrate solution 1 (first three drops of the solution); FS2 – filtrate solution 2 (the rest of the solution); MCE – mixed cellulose ester; NY – nylon; PES – polyethersulphone; PTFE – polytetrafluoroethylene

Figure 6

Relative recovery of the “outlier” mycotoxins AFB2 (a) and HT-2 (b) from filtrate solutions 1 and 2 by filter membrane materials. CA – cellulose acetate; FS1 – filtrate solution 1 (first three drops of the solution); FS2 – filtrate solution 2 (the rest of the solution); MCE – mixed cellulose ester; NY – nylon; PES – polyethersulphone; PTFE – polytetrafluoroethylene
Relative recovery of the “outlier” mycotoxins AFB2 (a) and HT-2 (b) from filtrate solutions 1 and 2 by filter membrane materials. CA – cellulose acetate; FS1 – filtrate solution 1 (first three drops of the solution); FS2 – filtrate solution 2 (the rest of the solution); MCE – mixed cellulose ester; NY – nylon; PES – polyethersulphone; PTFE – polytetrafluoroethylene

MS/MS parameters for mycotoxin analysis

MycotoxinPrecursor ionm/zCone voltageVCollision energyVProduct ionsm/z
AFB1313.2 (M+H)+6038241.0
23285.3
AFB2315.2 (M+H)+6030259.0
25287.0
AFG1329.2 (M+H)+6028311.0
24243.0
AFG2331.2 (M+H)+6024313.0
28245.1
DON297.2 (M+H)+2510249.0
10203.2
FB1722.4 (M+H)+5040334.3
40352.3
FB2706.4 (M+H)+5040336.2
40318.2
ZEA317.1 (M–H)--5830131.0
20175.0
T-2484.7 (M+NH4)+2520185.0
25215.0
HT-2442.6 (M+NH4)+2510263.4
15215.3
OTA404.1 (M+H)+3024239.0
14358.0

Preparation of the multi-mycotoxin standard solution

AnalyteConcentration of analytical standard (μ/mL)Volume of analytical standard for preparation of 1000 μL of combined multi-mycotoxin standard solution* (μL)Concentration of combined multi-mycotoxin standard solution (ng/mL)Concentration of 100× diluted combined multi-mycotoxin standard solution (ng/mL)
DON10050500050
AFB12.025500.5
AFB20.52512.50.125
AFG12.025500.5
AFG20.52512.50.125
FB15075375037.5
FB25075375037.5
ZEA1007.57507.5
T-21002.52502.5
HT-21002.52502.5
OTA102.5250.25
Volume of combined multi-mycotoxin standard solution for preparation of 1000 μL of 100× diluted combined multi-mycotoxin standard solution** (μL)10
DOI: https://doi.org/10.2478/aiht-2023-74-3693 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 8 - 15
Submitted on: Nov 1, 2022
Accepted on: Mar 1, 2023
Published on: Apr 4, 2023
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
filter-analyte interaction,
liquid chromatography-tandem mass spectrometry,
underestimation,
sample preparation
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2023 Marija Kovač Tomas, Anto Mijatović, Mateja Bulaić Nevistić, Bojan Šarkanj, Jurislav Babić, Tihomir Kovač, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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