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The effect of low doses of chlorpyrifos on blood and bone marrow cells in Wistar rats Cover

The effect of low doses of chlorpyrifos on blood and bone marrow cells in Wistar rats

Open Access
|Sep 2022

Figures & Tables

Figure 1

Effect of sub-chronic oral 28-day exposure to chlorpyrifos (CPF) on body weight gain (%, mean ± standard deviation) in adult male Wistar rats (N=5 per group). NC – negative control (NC); PC – positive control (ethyl methane sulphonate); SC – solvent control (0.03 % ethanol). * – significantly different from NC; $ – significantly different from SC (p<0.05)

Figure 2

Number of reticulocytes (mean ± standard deviation) per 1000 erythrocytes in adult male Wistar rats (N=5 per group) after sub-chronic oral 28-day exposure to chlorpyrifos. NC – negative control (NC); PC – positive control (ethyl methane sulphonate); SC – solvent control (0.03 % ethanol). * – significantly different from NC; b – significantly different from 0.015 mg/kg bw/day of CPF; c – significantly different from 0.015 mg/kg bw/day of CPF (p<0.05)

Figure 3

Number of micronuclei (mean ± standard deviation) in 2000 polychromatic erythrocytes in adult male Wistar rats (N=5 per group) after sub-chronic oral 28-day exposure to chlorpyrifos. NC – negative control (NC); PC – positive control (ethyl methane sulphonate); SC – solvent control (0.03 % ethanol). * – significantly different from NC; b– significantly different from 0.015 mg/kg bw/day of CPF (p<0.05)

Figure 4

Number of micronuclei (mean ± standard deviation) in 4000 reticulocytes in adult male Wistar rats (N=5 per group) after sub-chronic oral 28-day exposure to chlorpyrifos (CPF). NC – negative control (NC); PC – positive control (ethyl methane sulphonate); SC – solvent control (0.03 % ethanol). ↑ – significantly different from all experimental groups (p<0.05)

Figure 5

Photomicrograph of DNA damage in the bone marrow of adult male Wistar rats exposed to low doses chlorpyrifos by oral gavage for 28 consecutive days. The three bright nucleoids show low primary DNA damage; white arrow indicates a long-tailed nucleoid with extensive DNA damage. Letter c indicates a diffuse nucleoid with DNA damage possibly related to cytotoxicity

Effects of sub-chronic, oral, 28-day exposure to chlorpyrifos on primary DNA damage (tail DNA %) in bone marrow cells of adult male Wistar rats (N=5 per group) assessed with the alkaline comet assay

Experimental groupTail intensity (DNA %)
Negative control3.00±0.12
0.47
0–27.83

Positive control (ethyl methane sulphonate)16.33±0.32*
15.33
0–47.55

Solvent control (0.03 % ethanol)3.58±0.15
0.46
0–31.96

CPF 0.010 mg/kg bw/day3.09±0.12
0.48
0–30.68

CPF 0.015 mg/kg bw/day3.21±0.14
0.42
0–38.85

CPF 0.160 mg/kg bw/day2.70±0.13
0.31
0–54.96
DOI: https://doi.org/10.2478/aiht-2022-73-3665 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
Language: English, Croatian, Slovenian
Page range: 223 - 232
Submitted on: Jun 1, 2022
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Accepted on: Sep 1, 2022
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Published on: Sep 30, 2022
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2022 Vilena Kašuba, Vedran Micek, Mirta Milić, Davor Želježić, Anja Katić, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.