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Prenatal and perinatal phthalate exposure is associated with sex-dependent changes in hippocampal miR-15b-5p and miR-34a-5p expression and changes in testicular morphology in rat offspring Cover

Prenatal and perinatal phthalate exposure is associated with sex-dependent changes in hippocampal miR-15b-5p and miR-34a-5p expression and changes in testicular morphology in rat offspring

Archives of Industrial Hygiene and Toxicology
Volume 73 (2022): Issue 3 (September 2022)
By: Peter Štefánik,  Jaroslav Michalec,  Martina Morová,  Lucia Olexová and  Lucia Kršková  
Open Access
|Sep 2022

Figures & Tables

Figure 1

Comparison of luminal area cryosections of testes stained with Gill’s haematoxylin and eosin between a control and a phthalate-treated male rat
Comparison of luminal area cryosections of testes stained with Gill’s haematoxylin and eosin between a control and a phthalate-treated male rat

Figure 2

Comparison of representative micrographs of miR-15b-5p positive signals (green) in the hippocampal cornus ammonis 2 (CA2) region between a control and a phthalate-treated female rat
Comparison of representative micrographs of miR-15b-5p positive signals (green) in the hippocampal cornus ammonis 2 (CA2) region between a control and a phthalate-treated female rat

Figure 3

Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). Sex × treatment interaction is significant (F1,25=8.3501, p=0.0078). ** p<0.01 and # p<0.05 vs respective male and female controls (unpaired Student’s t-test: t=-2.778, p=0.017)
Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). Sex × treatment interaction is significant (F1,25=8.3501, p=0.0078). ** p<0.01 and # p<0.05 vs respective male and female controls (unpaired Student’s t-test: t=-2.778, p=0.017)

Figure 4

Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect (F1,25=4.9967, p=0.0345) and treatment effect (F1,25=4.7352, p=0.0392) are significant. * p<0.05 and # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.459, p=0.03)
Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect (F1,25=4.9967, p=0.0345) and treatment effect (F1,25=4.7352, p=0.0392) are significant. * p<0.05 and # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.459, p=0.03)

Figure 5

Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect shows a trend towards significance (F1,25=4.0915, p=0.0539). $ p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.529, p=0.022); # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.758, p=0.017)
Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect shows a trend towards significance (F1,25=4.0915, p=0.0539). $ p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.529, p=0.022); # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.758, p=0.017)

Figure 6

Relative number of miR-15b-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). ## p<0.01 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-3.143, p=0.008)
Relative number of miR-15b-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). ## p<0.01 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-3.143, p=0.008)

Figure 7

Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)
Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)

Figure 8

Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)
Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)

Figure 9

Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). The treatment effect (F1,19=5.009, p=0.037) and the sex × treatment interaction (F1,19=4.39, p=0.049) are significant. ** p<0.001 and # p<0.05 for the Pht m and Pht f comparison (unpaired Student’s t-test: t=2.363, p=0.042)
Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). The treatment effect (F1,19=5.009, p=0.037) and the sex × treatment interaction (F1,19=4.39, p=0.049) are significant. ** p<0.001 and # p<0.05 for the Pht m and Pht f comparison (unpaired Student’s t-test: t=2.363, p=0.042)

Figure 10

Relative number of miR-34a-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). # p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.476, p=0.028)
Relative number of miR-34a-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). # p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.476, p=0.028)

Comparison of sperm-producing duct areas (μm2) between control (n=12) and phthalate-treated (n=12) male rats_ Values are presented as means±standard deviations (SD)

ItemsControl (Mean±SD)Phthalates (Mean±SD)p value
Channel area (μm2)100292±3768.6103586±5053.50.595936
Inner duct area (μm2)19419±176925151±1433.30.019613
Inner duct area / channel area (μm2)188091±1430241257±11300.008059
DOI: https://doi.org/10.2478/aiht-2022-73-3641 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 191 - 199
Submitted on: Mar 1, 2022
Accepted on: Sep 1, 2022
Published on: Sep 30, 2022
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
dentate gyrus,
di-(2-ethylhexyl)phthalate,
di-isononyl phthalate,
di-n-butyl phthalate,
microRNA
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2022 Peter Štefánik, Jaroslav Michalec, Martina Morová, Lucia Olexová, Lucia Kršková, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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