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![Migration of A. junii (A) and A. baumannii (B) from the air/liquid interface to the air-exposed section of the glass slide. EPS is dyed in blue/purple to better show spreading up the slide [A. junii (C); A. baumannii (D)]](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/64708df871e4585e08a9fe5e/j_aiht-2020-71-3436_fig_009.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=AKIA6AP2G7AKOUXAVR44%2F20251205%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20251205T095335Z&X-Amz-Expires=3600&X-Amz-Signature=a106c1d6d2e87c8d7bda3174ef563696d6a025057c466fc4873940fb022581d1&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
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Swarming and twitching motility determined by standard assays for B_ cereus, A_ junii, A_ baumannii and P_ aeruginosa and expressed as diameter of the growth area from inoculation spot
| B. cereus | A. junii | A. baumannii | P. aeruginosa | |
|---|---|---|---|---|
| Swarming | 82±14 mm | N/A | N/A | 16±7 mm |
| Twitching | N/A | 35±12 mm | 45±14 mm | 51±9 mm |
Experimental combinations performed in order to explain the migration of bacterial cells up the glass slide
| Biofilm growth (days) | B. cereus | A. baumannii | |||||
|---|---|---|---|---|---|---|---|
| 103 CFU/mL | 107 CFU/mL | 103 CFU/mL | 107 CFU/mL | Carbol-fuchsin | |||
| 1 | Exp 1 | Exp 2 | Exp 17 | Exp 18 | Exp 33 | ||
| LB medium | 3 | Exp 3 | Exp 4 | Exp 19 | Exp 20 | Exp 34 | |
| 7 | Exp 5 | Exp 6 | Exp 21 | Exp 22 | Exp 35 | ||
| 10 | Exp 7 | Exp 8 | Exp 23 | Exp 24 | Exp 36 | ||
| 1 | Exp 9 | Exp 10 | Exp 25 | Exp 26 | Exp 37 | ||
| 3 | Exp 11 | Exp 12 | Exp 27 | Exp 28 | Exp 38 | ||
| Sterile saline (0.3 %) | 7 | Exp 13 | Exp 14 | Exp 29 | Exp 30 | Exp 39 | |
| 10 | Exp 15 | Exp 16 | Exp 31 | Exp 32 | Exp 40 | ||