Biomonitoring findings for occupational lead exposure in battery and ceramic tile workers using biochemical markers, alkaline comet assay, and micronucleus test coupled with fluorescence in situ hybridisation Cover

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Biomonitoring findings for occupational lead exposure in battery and ceramic tile workers using biochemical markers, alkaline comet assay, and micronucleus test coupled with fluorescence in situ hybridisation

Archives of Industrial Hygiene and Toxicology

Volume 71 (2020): Issue 4 (December 2020)

By: Vilena Kašuba, Mirta Milić, Davor Želježić, Marin Mladinić, Alica Pizent, Zorana Kljaković-Gašpić, Melita Balija and Irena Jukić

Open Access

|Dec 2020

Figures & Tables

General characteristics of lead-exposed workers and controls by gender and smoking

		Exposed group				Control group
Parameters (mean±SE)	Women (N=17)	Men (N=33)	Smokers (N=33)	Non-smokers (N=17)	Women (N=18)	Men (N=30)	Smokers (N=26)	Non-smokers (N=22)
Age (years)	36±2.57	40±1.57	36.55±1.75	41.8242.0	35.17±2.49	40.73±1.87	37.15±2.27	40.41±1.98
Years of exposure	5.05±1.42	13.39±1.60	9.98±1.51	11.07±2.34	0	0	0	0
B-(μPB g/L)	228.13±25.20^c,d	340.33±30.98^e	299.23±27.08^g	296.66±43.42^h	28.2±3.49^b	47.16±5.01	40.49±6.11	43.14±4.22
ALAD (U/L ercs)	28.21±3.48^d	20.92±2.91^e	22.12±2.63^f,g	25.65±4.24^h	55.84±2.81	56.61±2.61	57.85±2.62	53.27±2.15
EP (μmol/L ercs)	2.11±0.44^e	3.59±0.71^e	2.89±0.57^g	3.3540.92^"	0.98±0.08	0.98±0.06	1.03±0.07	0.9±0.06
S-B ₁₂ (μg/L)	410.14±35.82^d	467.55±25.81	445.93±26.69	446.93±33.05	537.33±46.75	434.53±24.52	447.38±30	503.45±38.38
S-folate (ng/L)	6.41±0.66^b,d	5.61±0.42	5.26±0.38^f,g	7.0640.62^"	9.11±0.86	6.84±0.64	7.18±0.56	8.30±0.96

MN-FISH assay endpoints in lead-exposed and control subgroups by gender and smoking status

		Exposed group				Control group
Parameters (mean±SE)	Women (N=8)	Men (N=12)	Smokers (N=12)	Non-smokers (N=8)	Women (N=5)	Men (N=11)	Smokers (N=10)	Non-smokers (N=6)
MN_total	22.38±2.33	22.92±3.53	24.08±3.17	20.14±2.78	6.6±1.4^c	9.00±1.00^d	9.8±1.03^e,f	5.67±0.56^g
C+ MN_total	9.25±2.13^b	7.42±1.38	8.46±1.30	7.57±2.45	2.4±0.4^c	4.27±0.59	4.3±0.62^r	2.67±0.56^g
C- MN_total	13.13±2.00	15.5±2.72	15.62±2.52	12.57±2.17	4.2±1.46^c,d	4.73±1.15	5.5±1.36^f	3.00±0.00^g
NB_total	2.88±0.61^b	2.67±0.81	2.85±0.77	2.57±0.61	2.4±1.12	5.00±1.33	5±1.48	2.83±1.01
C+NB_total	0.13±0.13	0.5±0.34	0.46±0.31	0.14±0.14	0.2±0.2	1±0.38	1±0.42	0.33±0.21
C-NB_total	2.75±0.56	2.17±0.58	2.38±0.57	2.43±0.53	2.2±0.97	4±0.96	4±1.06	2.5±0.85
NPB_total	1.5±0.42	2±0.85	1.69±0.58	2±1.13	0.6±0.4	0.27±0.14	0.5±0.22	0.17±0.17
C+NPB_total	0.13±0.13	0	0.08±0.08	0	0	0	0	0
C-NPB_total	1.38±0.46	2±0.85	1.62±0.59	2±1.13	0.6±0.4	0.27±0.14	0.5±0.22	0.17±0.17

General characteristics of lead-exposed workers and controls

Parameters (mean ± SE)	Exposed group (N=50)	Control group (N=48)
Age (years)	38.34±9.71	38.65±1.53
Years of exposure	10.36±8.94	0
B-PB (μg/L)	298.36±162.07	41.58±23.02
ALAD (U/L ercs)	23.32±15.87	56.32±9.92
EP (μmol/L ercs)	3.05±3.43	0.98±0.27
S-B₁₂ (ng/L)	446.25±137.98	473.08±166.99
S-folate (ng/L)	5.83±2.31	7.69±3.70

Micronucleus assay and alkaline comet assay endpoints in lead-exposed workers and controls by gender and smoking

Parameters (mean±SE)	Total MNi	NDI	TL	TI	Total NBs	Total NPBs	NPB/MN ratio
Exposed (N=50)	18.71±11.06^a	2.17±0.23^a	16.15±5.33	2.64±3.22	3.65±5.91	2.02±2.47^a	0.12±0.02
Controls (N=48)	8.98±7.50	2.13±0.25	14.27±1.23	1.61±0.74	2.29±2.34	1.14±1.53	0.26±0.08
Control (N=18) W	8.11±1.72	2.22±0.06	13.67±0.21^b	1.34±0.16^b
Control (N=30) M	9.5±1.41	2.07±0.05	14.67±0.25	1.79±0.14
Exposed (N=17) W	20.06±2.09^d	2.16±0.07	16.19±1.28^d	3.04±0.92^d
Exposed (N=33) M	18.4±2.23^e	2.16±0.05^e	16.20±0.99	2.46±0.53
Control N=(26) S	9.27±1.59	2.13±0.05
Control (N=22) NS	8.64±1.47	2.14±0.05
Exposed (N=33) S	17.87±2.07^f,g	2.21±0.04^f,g
Exposed (N=17) NS	20.24±2.49^h	2.09±0.08^h

MN-FISH assay endpoints in lead-exposed and control subgroups

Parameters (mean±SE)	Exposed group N=20	Control group N=16
Age (years)	39.6±2.05	40.31±1.59
Gender	8 women 12 men	5 women 11 men
Smoking	12 smokers 8 non-smokers	10 smokers 6 non-smokers
Time of exposure (years)	11.66±1.91	0
MN_total	22.70±2.27	8.25±0.84
C+ MN_total	8.15±1.80	3.69±0.47
C- MN_total	14.55±1.80	4.56±0.89
NB_total	2.75±0.53	4.18±1.01
C+ NB_total	0.35±0.21	0.75±0.28
C- NB_total	2.40±0.41	3.43±0.74
NPB_total	1.80±0.53	0.38±0.15
C+ NPB_total	0.05±0.05	0
C- NPB_total	1.75±0.54	0.38±0.15

DOI: https://doi.org/10.2478/aiht-2020-71-3427 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254

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Language: English, Croatian, Slovenian

Page range: 339 - 352

Submitted on: May 1, 2020

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Accepted on: Nov 1, 2020

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Published on: Dec 31, 2020

Published by: Institute for Medical Research and Occupational Health

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

genetic endpoints,

human lymphocytes,

Related subjects:

Basic medical science,

Basic medical science, other

© 2020 Vilena Kašuba, Mirta Milić, Davor Želježić, Marin Mladinić, Alica Pizent, Zorana Kljaković-Gašpić, Melita Balija, Irena Jukić, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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