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Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture Cover

Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture

Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Issue 3 (September 2020)
By: Dragana Javorac,  Aleksandra Buha Đorđević,  Milena Anđelković,  Simona Tatović,  Katarina Baralić,  Evica Antonijević,  Jelena Kotur-Stevuljević,  Danijela Đukić-Ćosić,  Biljana Antonijević and  Zorica Bulat  
Open Access
|Oct 2020

Figures & Tables

Figure 1

Brain Pb levels in Wistar rats after acute exposure to Pb alone and in combination with Cd (N=6–8 per group). *** P<0.001 compared to control (one-way ANOVA followed by LSD). Pb 150 – the group treated with Pb in the dose of 150 mg/kg bw; Cd15+Pb150 – the group treated with a combination of 150 mg/kg Pb and 15 mg/kg of Cd. Boxes represent interquartile ranges (25–75th percentile), the line within the box median values, and the ends of the whiskers minimum and maximum values within the group
Brain Pb levels in Wistar rats after acute exposure to Pb alone and in combination with Cd (N=6–8 per group). *** P<0.001 compared to control (one-way ANOVA followed by LSD). Pb 150 – the group treated with Pb in the dose of 150 mg/kg bw; Cd15+Pb150 – the group treated with a combination of 150 mg/kg Pb and 15 mg/kg of Cd. Boxes represent interquartile ranges (25–75th percentile), the line within the box median values, and the ends of the whiskers minimum and maximum values within the group

Figure 2

Brain Cd levels in Wistar rats after acute exposure to Cd alone and in combination with Pb (N=6–8 per group). * P<0.05, ** P<0.01, *** P<0.001 compared to control; † P<0.05, †† P<0.01 compared to Cd15; ‡ p<0.05, compared to Cd30 (one-way ANOVA followed by LSD). Cd15 – the group treated with Cd in the dose of 15 mg/kg bw; Cd30 – the group treated with Cd in the dose of 30 mg/kg bw; Cd15+Pb150 – the group treated with a combination of 150 mg/kg Pb and 15 mg/kg Cd. Boxes represent interquartile ranges (25–75th percentile), the line within the box median value, and the ends of the whiskers minimum and maximum values within the group
Brain Cd levels in Wistar rats after acute exposure to Cd alone and in combination with Pb (N=6–8 per group). * P<0.05, ** P<0.01, *** P<0.001 compared to control; † P<0.05, †† P<0.01 compared to Cd15; ‡ p<0.05, compared to Cd30 (one-way ANOVA followed by LSD). Cd15 – the group treated with Cd in the dose of 15 mg/kg bw; Cd30 – the group treated with Cd in the dose of 30 mg/kg bw; Cd15+Pb150 – the group treated with a combination of 150 mg/kg Pb and 15 mg/kg Cd. Boxes represent interquartile ranges (25–75th percentile), the line within the box median value, and the ends of the whiskers minimum and maximum values within the group

Brain Zn, Cu, Mn, and Fe levels in Wistar rats after acute exposure to Pb and/or Cd (N=6–8 per group)

Groups
Essential metalsControl (μg/g)Cd15 (μg/g)Cd30 (μg/g)Pb150 (μg/g)Cd15+Pb150 (μg/g)
Zn11.1210.69–15.1012.3511.39–13.7112.6812.12–15.6612.7410.87–14.8914.8411.16–15.40
Cu1.99±0.412.24±0.272.17±0.232.26±0.261.88±0.22
Mn1.93±0.441.38±0.241.72±0.341.65±0.401.90±0.51
Fe22.4215.52–26.3518.1216.08–30.8119.3115.85–33.6121.1919.12–22.8616.8713.44–24.93

Effects of acute Pb and/or Cd treatment on oxidative stress parameters in the brain of Wistar rats (N=6–8 per group)

Groups
ParameterControlCd15Cd30Pb150Cd15+Pb150
TBARS (μmol/mg protein)161.36±47.74175.25±43.23309.64±106.78** †230.95±47.82390.57±95.15*** †† §
AOPP (μmol/min/g protein)27.57±6.2824.76±4.9027.68±6.4325.4±6.1824.83±8.83
O2·− (μmol/min/g protein)91.74±6.6599.78±17.3091.38±18.9495.13±11.8290.24±7.92
SOD (U/g)57.35±10.6347.52±5.0357.73±18.8258.29±5.8749.32±10.92
SH(mmol/g protein)0.14±0.030.15±0.020.13±0.020.15±0.020.12±0.02
TAS(μmol Trolox Equiv. /g protein)130.00 87.70–188.28268.66* 232.28–368.51300.07*** 274.71–346.72222.61** ‡‡ 108.48–336.72264.91** 221.20–274.67
TOS(μmol H2O2 Equiv. /g protein)2.36±0.682.46±0.532.05±0.542.77±0.652.89±1.89
OSI0.0162±0.00780.0106±0.0049*0.0068±0.0016**0.0149±0.0087‡0.0099±0.0037*
DOI: https://doi.org/10.2478/aiht-2020-71-3425 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 197 - 204
Submitted on: Apr 1, 2020
Accepted on: Sep 1, 2020
Published on: Oct 6, 2020
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Cd,
Cu,
Fe,
MDA,
Mn,
neurotoxicity,
oxidative stress,
Pb,
TBARS,
Zn
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2020 Dragana Javorac, Aleksandra Buha Đorđević, Milena Anđelković, Simona Tatović, Katarina Baralić, Evica Antonijević, Jelena Kotur-Stevuljević, Danijela Đukić-Ćosić, Biljana Antonijević, Zorica Bulat, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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