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Cherry laurel fruit extract counters dimethoate-induced reproductive impairment and testicular apoptosis Cover

Cherry laurel fruit extract counters dimethoate-induced reproductive impairment and testicular apoptosis

Open Access
|Dec 2020

Figures & Tables

Figure 1

Light microscopy of testicular tissue in the experimental groups (H&E, 20x). (A) normal seminiferous tubular morphology in control rats; (B) damaged testicular structure in dimethoate-treated rats (arrow head shows the lumens of seminiferous tubules containing fewer elongated spermatids; arrows show haemorrhage; asterisks show desquamation in germinal cells); (C) normal testis structure in rats treated with cherry laurel extract (CLE); (D) histology of the testicular tissue of rats pre-treated with CLE before receiving dimethoate; (E) normal seminiferous tubule morphology in the vitamin C-treated rats receiving dimethoate; (F) rare degenerated tubules and histopathological changes in rats receiving post-treatment with CLE
Light microscopy of testicular tissue in the experimental groups (H&E, 20x). (A) normal seminiferous tubular morphology in control rats; (B) damaged testicular structure in dimethoate-treated rats (arrow head shows the lumens of seminiferous tubules containing fewer elongated spermatids; arrows show haemorrhage; asterisks show desquamation in germinal cells); (C) normal testis structure in rats treated with cherry laurel extract (CLE); (D) histology of the testicular tissue of rats pre-treated with CLE before receiving dimethoate; (E) normal seminiferous tubule morphology in the vitamin C-treated rats receiving dimethoate; (F) rare degenerated tubules and histopathological changes in rats receiving post-treatment with CLE

Figure 2

Apoptotic germ cells (arrows) in testicular tissues (H&E, 400x). (A) the control group; (B) in the dimethoate alone group, many TUNEL-positive apoptotic cells were found; (C) cherry laurel extract (CLE) alone group; (D) CLE pre-treatment decreased the number of TUNEL-positive apoptotic cells in rats receiving dimethoate; (E) vitamin C-treated group receiving dimethoate; (F) CLE post-treatment group receiving dimethoate
Apoptotic germ cells (arrows) in testicular tissues (H&E, 400x). (A) the control group; (B) in the dimethoate alone group, many TUNEL-positive apoptotic cells were found; (C) cherry laurel extract (CLE) alone group; (D) CLE pre-treatment decreased the number of TUNEL-positive apoptotic cells in rats receiving dimethoate; (E) vitamin C-treated group receiving dimethoate; (F) CLE post-treatment group receiving dimethoate

Effects of different treatments on relative reproductive organ weights in rats

GroupsRelative reproductive organ weights*
Testis (Right+left/2)Whole epididymis (Right+left/2)Right cauda epididymisSeminal vesicleVentral prostate
Control0.433±0.009bc0.167±0.005ab0.065±0.001ab0.369±0.017b0.176±0.007a
Dimethoate0.379±0.014d0.113±0.007c0.047±0.003c0.328±0.012c0.129±0.005b
CLE0.456±0.013abc0.171±0.006a0.069±0.002a0.408±0.014ab0.184±0.010a
CLEpre + dimethoate0.474±0.041ab0.171±0.005a0.064±0.001ab0.448±0.014a0.166±0.003a
Vitamin C + dimethoate0.477±0.016a0.172±0.004a0.066±0.001ab0.451±0.007a0.175±0.003a
Dimethoate + CLEpost0.430±0.014c0.146±0.002b0.060±0.001b0.388±0.004b0.166±0.003a
Median0.4420.1610.0630.3900.165
First quartile0.4170.1460.0600.3750.156
Third quartile0.4690.1740.0680.4430.183
Interquartile range0.0600.0280.0080.0630.025

Effects of different treatments on whole body and absolute reproductive organ weights in rats

GroupsBody (weight g)Absolute reproductive organ weights (g)
Testis (Right+left/2)Whole epididymis (Right+left/2)Right cauda epididymisSeminal vesicleVentral prostate
Control310.30±6.19a1.339±0.003a0.516±0.035a0.202±0.001a1.137±0.039a0.544±0.019a
Dimethoate233.75±7.64b0.878±0.012d0.260±0.010d0.111±0.007d0.761±0.007d0.298±0.003d
CLE308.00±9.22a1.394±0.021a0.521±0.002a0.210±0.001a1.245±0.002b0.562±0.025a
CLEpre + dimethoate243.67±4.79b1.151±0.027b0.416±0.008b0.157±0.001b1.090±0.025a0.402±0.002bc
Vitamin C + dimethoate253.70±2.51b1.209±0.003b0.437±0.008b0.168±0.002b1.142±0.009a0.442±0.007b
Dimethoate + CLEpost235.44±2.27b1.012±0.030c0.344±0.005c0.139±0.001c0.913±0.010c0.374±0.002c
Median254.501.2050.4300.1631.1150.415
First quartile235.221.0150.3430.1390.9170.375
Third quartile303.001.3400.5200.2031.2400.540
Interquartile range67.890.330.180.060.290.16

Effects of different treatments on malondialdehyde (MDA) levels and antioxidant enzyme activities in testicular tissue of rats

GroupsParameters
MDA (nmol/mg protein)SOD (U/mg protein)GPx (U/mg protein)CAT (U/mg protein)
Control0.341±0.021x3.676±0.105a0.331±0.009A5.257±0.250
Dimethoate0.486±0.021y3.174±0.090b0.265±0.012B4.291±0.244
CLE0.386±0.017x3.635±0.078a0.335±0.017A4.068±0.282
CLEpre + dimethoate0.377±0.015x3.603±0.066a0.326±0.014A4.681±0.310
Vitamin C + dimethoate0.362±0.012x3.662±0.079a0.327±0.014A4.828±0.257
Dimethoate + CLEpost0.367±0.028x3.614±0.114a0.329±0.017A5.114±0.294
Median0.3803.6500.3204.700
First quartile0.3303.3200.2903.945
Third quartile0.4303.8050.3555.360
Interquartile range0.100.490.071.42

Effects of different treatments on semen parameters, DNA damage, and testicular apoptosis in rats

Semen parameters
GroupsMotility (%)Concentration (million/right cauda epididymis)Abnormal sperm rate (%)Sperm DNA damage (%)Testicular apoptosis (%)
HeadTailTotal
Control74.34±1.09b110.40±2.04b3.60±0.40d6.10±0.43c9.70±0.52d8.47±0.10c1.57±0.28c
Dimethoate31.25±2.52e46.25±1.59e22.63±0.42a20.13±0.72a42.76±0.96a11.36±0.18a6.07±0.42a
CLE83.17±1.28a121.40±1.08a3.30±0.30d5.20±0.39c8.50±0.37d8.98±0.13c1.86±0.30c
CLEpre + dimethoate54.44±1.39cd91.78±1.35c7.67±0.44c8.78±0.40b16.45±0.56c10.12±0.14b2.70±0.36b
Vitamin C + dimethoate56.67±1.31c94.11±1.95c8.33±0.58c9.22±0.52b17.55±0.90bc10.82±0.16a2.33±0.40bc
Dimethoate + CLEpost50.19±1.07d77.11±1.14d11.33±0.54b8.89±0.43b20.22±0.63b11.23±0.14a2.17±0.53bc
Median55.0094.008.008.0016.0010.001.00
First quartile50.0076.284.006.0010.007.000.00
Third quartile75.84112.0011.0010.0020.1613.005.00
Interquartile range26.2535.727.004.0010.166.005.00
DOI: https://doi.org/10.2478/aiht-2020-71-3412 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
Language: English, Croatian, Slovenian
Page range: 329 - 338
Submitted on: Mar 1, 2020
Accepted on: Oct 1, 2020
Published on: Dec 31, 2020
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2020 Elçin Bakır, Serpil Sarıözkan, Burcu Ünlü Endirlik, Ayşe Baldemir Kılıç, Arzu Hanım Yay, Fazile Cantürk Tan, Ayşe Eken, Gaffari Türk, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.