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Superior protective effects of in vitro propagated green garlic against hydrogen peroxide-induced cytotoxicity in human hepatoma cells Cover

Superior protective effects of in vitro propagated green garlic against hydrogen peroxide-induced cytotoxicity in human hepatoma cells

Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Issue 2 (June 2020)
By: Tomislav Vinković,  Nada Parađiković,  Monika Tkalec Kojić,  Gordana Mendaš,  Tanja Živković Semren,  Valentina Gluščić,  Ivana Vinković Vrček and  Ivan Pavičić  
Open Access
|Jun 2020

Figures & Tables

Figure 1

Total garlic extract phenolic content (expressed as mg of GAE per gram of garlic powder) and antioxidant activity (AOX, expressed as mm of TEAC per gram of garlic powder). Values represent means ± standard deviations. Different letters denote significant differences (P<0.05) between CG and PG. GAE – gallic acid equivalent; TEAC – Trolox equivalent antioxidant activity

Figure 2

Total flavonoid and alliin content (expressed as milligrams of quercetin and alliin per gram of garlic powder, respectively). Values represent means ± standard deviations. Different letters denote significant differences (P<0.05) between CG and PG

Figure 3

Hepatoma cell viability according to the WST-8 colorimetric assay. Data are expressed as a percentage of absorbance with respect to negative control as a starting point (100 %). Values represent means ± standard deviations. Values marked with the same letters are not significantly different, whereas values marked with different letters are significantly different. # indicates significant difference from negative control and * from positive (H2O2) control (P<0.05)

Figure 4

GSH levels determined with the MBCI fluorescent probe. Data are expressed as a percentage of fluorescence of negative control as a starting point (100 %). Values represent means ± standard deviations. Values marked with the same letters are not significantly different, whereas values marked with different letters are significantly different. # indicates significant difference from negative control and * from positive (H2O2) control (P<0.05)

Figure 5

Intracellular ROS levels determined with the DCFH-DA fluorescent probe. Data are expressed as a percentage of fluorescence of negative control as a starting point (100 %). Values represent means ± standard deviations. Values marked with the same letters are not significantly different, whereas values marked with different letters are significantly different. # indicates significant difference from negative control and * from positive (H2O2) control (P<0.05)

Differences in yield and overall alliin content between conventional growing and in vitro propagation

Garlic PTLeaves numberYield per plant (bulbs or leafy part) (g)Yield per square meter (g)Alliin yield per square meter (g)
CG12.57±0.89a38.69±0.73a1102.77±20.89b9.02±0.15b
PG9.89±0.66b30.57±0.42b3056.98±42.14a74.79±0.94a
DOI: https://doi.org/10.2478/aiht-2020-71-3399 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 130 - 137
Submitted on: Feb 1, 2019
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Accepted on: Jun 1, 2020
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Published on: Jun 29, 2020
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Allium sativum L.,
alliin,
antioxidant activity,
glutathione,
total phenolic content,
total flavonoid content
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2020 Tomislav Vinković, Nada Parađiković, Monika Tkalec Kojić, Gordana Mendaš, Tanja Živković Semren, Valentina Gluščić, Ivana Vinković Vrček, Ivan Pavičić, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution 4.0 License.

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