Cytotoxic and mutagenic potential of juglone: a comparison of free and nano-encapsulated form Cover

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Cytotoxic and mutagenic potential of juglone: a comparison of free and nano-encapsulated form

Archives of Industrial Hygiene and Toxicology

Volume 71 (2020): Issue 1 (March 2020)

By: Semiha Erisen, Tülin Arasoğlu, Banu Mansuroglu, İsmail Kocacaliskan and Serap Derman

Open Access

|Apr 2020

Figures & Tables

Chemical structure of juglone (A) and poly (lactic-co-glycolic acid) (B)

Particle size distribution (A), scanning electron microscopy image (B), FT-IR spectra (C), and release pattern (D) of nanoparticles

Viability of L929 fibroblasts treated with free and PLGA nanoparticle-coated juglone (JNP) after 24 h (A) and 48 h (B); Cell viability was measured with the MTT assay. Values are expressed as means of quadruple samples with standard error. *statistically significant differences between juglone and JNP (p<0.05)

Viability of alfalfa cells treated with free and PLGA nanoparticle-coated juglone (JNP) after 24 h (A) and 48 h (B); Cell viability was measured with the MTT assay. Values are expressed as means of triple samples with standard error. *statistically significant differences between juglone and JNP (p<0.05)

Potential mutagenicity in S. typhimurium TA98 treated with 0.31−10 μmol/L juglone: (A) TA98-S9 treated with JNPs; (B) TA98+S9 treated with JNPs; (C) TA98-S9 treated with free juglone; (D) TA98+S9 treated with free juglone; PC – positive control: 2-NF (2 μg/mL) and 4-NQO (0.1 μg/mL) without S9 and 2-AA (1 μg/mL) with S9; NC – negative control: water; *B value ≥0.99

Potential mutagenicity in S. typhimurium TA100 treated with 0.31−10 μmol/L juglone: (A) TA100-S9 treated with JNPs; (B) TA100+S9 treated with JNPs; (C) TA100-S9 treated with free juglone; (D) TA100+S9 treated with free juglone; PC – positive control: 2-NF (2 μg/mL) and 4-NQO (0.1 μg/mL) without S9 and 2-AA (2.5 μg/mL) with S9; NC – negative control: water; *B value ≥0.99

Physicochemical properties of juglone loaded PLGA nanoparticles

Encapsulating efficiency (%)	Drug loading (%)	Particle size (nm)	Zeta potential (mV)	Polydispersity index
29.95±2.8	15.42±2.1	213.33±5.2	-14.58±2.8	0.115±0.03

DOI: https://doi.org/10.2478/aiht-2020-71-3344 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254

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Language: English, Croatian, Slovenian

Page range: 69 - 77

Submitted on: Oct 1, 2019

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Accepted on: Feb 1, 2020

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Published on: Apr 9, 2020

Published by: Institute for Medical Research and Occupational Health

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

L929 fibroblasts,

Related subjects:

Basic medical science,

Basic medical science, other

© 2020 Semiha Erisen, Tülin Arasoğlu, Banu Mansuroglu, İsmail Kocacaliskan, Serap Derman, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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