Figure 1
Figure 2
Figure 3
Chromatographic parameters for beta-blockers separated on the Chirobiotic V column using the MeOH:HAc:TEA (100:0_2:0_15) mobile phase, flow rate of 0_5 mL/min, and column temperature of 45 °C (detection wavelength 230 nm)
| Analyte | Enantiomer | Retention time/min | Rs | k | α |
|---|---|---|---|---|---|
| E1 | 13.19 | 1.12 | |||
| Acebutolol | E2 | 13.99 | 1.43 | 1.24 | 1.12 |
| E1 | 14.53 | 1.33 | |||
| Atenolol | E2 | 15.38 | 1.37 | 1.46 | 1.10 |
| Bisoprolol | E1 | 10.85 | 1.30 | 0.74 | 1.12 |
| E2 | 11.43 | 0.83 | |||
| E1 | 17.49 | 0.65 | 1.77 | 1.03 | |
| Labetalol | E2 | 18.11 | 3.06 | 1.82 | 1.26 |
| E3 | 21.16 | 2.30 | |||
| E4 | 23.67 | 2.41 | 2.69 | 1.17 | |
| E1 | 11.43 | 0.84 | |||
| Metoprolol | E2 | 12.04 | 1.30 | 0.93 | 1.12 |
Expected and calculated concentrations of bisoprolol enantiomers and their recoveries in spiked samples of distilled and surface waters from Zagreb and Prague
| Expected γ (μg/L) | Calculated γ (μg/L) | Recovery (%) | ||||
|---|---|---|---|---|---|---|
| BIS 1 | BIS 2 | BIS 1 | BIS 2 | BIS 1 | BIS 2 | |
| Distilled water 1 | 12.00 | 12.00 | 12.02 | 11.85 | 100.2 | 98.8 |
| Distilled water 2 | 240.00 | 240.00 | 265.71 | 264.58 | 110.7 | 110.2 |
| Prague 1 | 12.00 | 12.00 | 13.18 | 13.19 | 109.8 | 109.9 |
| Prague 2 | 9.36 | 9.36 | 10.08 | 10.08 | 107.7 | 107.7 |
| Prague 3 | 8.16 | 8.16 | 9.44 | 9.49 | 115.7 | 116.3 |
| Sava 1 | 1.80 | 1.80 | 2.27 | 2.35 | 126.3 | 130.3 |
| Sava 2 | 6.00 | 6.00 | 8.14 | 8.30 | 135.7 | 138.3 |
| Sava 3 | 12.00 | 12.00 | 13.90 | 14.31 | 115.8 | 119.3 |