ISSR Fingerprints and ITS Restriction Cleavage Differs in Selected Native Chilli Pepper Genotypes
References
- Albrecht, E., Zhang, D., Saftner, R.A., & Stommel, J.R. (2012). Genetic diversity and population structure of Capsicum baccatum genetic resources. Genetic Resources and Crop Evolution, 59, 517–538. https://doi.org/10.1007/s10722-011-9700-y
- Alansi, S., Tarroum, M., Al-Qurainy, F., Khan, S., & Nadeem, M. (2016). Use of ISSR markers to assess the genetic diversity in wild medicinal Ziziphus spina-christi (L.) Willd. collected from different regions of Saudi Arabia. Biotechnology and Biotechnological Equipment, 30, 942–947. https://doi.org/10.1080/13102818.2016.1199287
- Amiteye, S. (2021). Basic concepts and methodologies of DNA marker systems in plant molecular breeding. Heliyon, 30, e08093. https://doi.10.1016/j.heliyon.2021.e08093
- Csilléry, G. (2006). Pepper taxonomy and the botanical description of the species. Acta Agronomica Hungarica, 54, 151–166. https://doi.org/10.1556/AAgr.54.2006.2.5
- da Costa, F.R., Pereira, T.N.S., Vitória, A.P., de Campos, K.P., Rodrigues, R., da Silva, D.H., & Pereira, M.G. (2006). Genetic diversity among Capsicum accessions using RAPD markers. Crop Breeding and Applied Biotechnology, 6, 18–23.
- dos Santos Pessoa, A.M., do Rago, E.R., dos Santos, C.A., & do Rago, M.M. (2018). Genetic diversity among accessions of Capsicum annuum L. through morphoagronomic characters. Genetics and Molecular Research, 17, gmr16039883. https://doi.org/10.4238/gmr16039883
- Gemmill, C.E.C., & Grierson, E.R.P. (2021). Inter-Simple Sequence Repeats (ISSR), Microsatellite-Primed Genomic Profiling Using Universal Primers. Methods of Molecular Biology, 2222, 49–262. https://doi.org.10.1007/978-1-0716-0997-2_14
- Gu, X.Z., Cao, Y.C., Zhang, Z.H., Zhang, B.X., Zhao, H., Zhang, X.M., Wang, H.P., Li, X.X., & Wang, L.H. (2019). Genetic diversity and population structure analysis of Capsicum germplasm accessions. Journal of Integrative Agriculture, 18, 1312–1320. https://doi.org/10.1016/S2095-3119(18)62132-X
- Gupta, M., Verma, B., Kumar, N., Chahota, R.K., Rathour, R., Sharma, S.K., Bhatia, S., & Sharma, T.R. (2012). Construction of intersubspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers. Journal of Genetics, 91, 279–287. https://doi.org/10.1007/s12041-012-0180-4
- Gupta, V., Jatav, P.K., Haq, S.U., Verma, K.S., Kaul, V.K., Kothari, S., & Kachhwaha, S. (2019). Translation initiation codon (ATG) or SCoT markers-based polymorphism study within and across various Capsicum accessions: insight from their amplification, cross-transferability and genetic diversity. Journal of Genetics, 98,1–12. https://doi.org/10.1007/s12041-019-1095-0
- Haq, S., Dubey, S., Dhingra, P. et al. (2022). Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights. Journal of Genetic Engeneering and Biotechnology, 20, 116. https://doi.org/10.1186/s43141-022-00398-1
- Hasan, M., Kulsum, M., Ullah, M., Hossain, M.M., & Mahmud, M.E. (2014). Genetic diversity of some chili (Capsicum annuum L.) genotypes. International Journal of Agricultural Research, Innovation and Technology, 4, 32–35. https://doi.org/10.3329/ijarit.v4i1.21088
- Qin, C., Yu, C., Shen, Y., Fang, X., Chen, L., Min, J., Cheng, J., Zhao, S., Xu, & M., Luo, Y. (2014). Whole-genome sequencing of cultivated and wild peppers provides insights into Capsicum domestication and specialization. Proceedings of National Academy of Sciences, 111, 5135–5140. https://doi.org/10.1073/pnas.1400975111
- Ibarra-Torres, P., Valadez-Moctezuma, E., Pérez-Grajales, M., Rodríguez-Campos, J., & Jaramillo-Flores, M.E. (2015). Inter-and intraspecific differentiation of Capsicum annuum and Capsicum pubescens using ISSR and SSR markers. Scientia Horticulturae, 181,137–146. https://doi.org/10.1016/jscienta.2014.10.054
- Jha, T.B., & Bhowmick, B.K. (2021). Conservation of floral, fruit and chromosomal diversity: a review on diploid and polyploid Capsicum annuum complex in India. Molecular Biology Reports, 48, 5587–5605. https://doi.org/10.1007/s11033-021-06355-4
- Kehie, M., Kumaria, S., Devi, K.S., & Tandon, P. (2015). Genetic diversity and molecular evolution of Naga King Chili inferred from internal transcribed spacer sequence of nuclear ribosomal DNA. Meta Gene, 2, 56–63. https://doi.org.10.1016/j.mgene.2015.11.006
- Lozada, D.N., Bhatta, M., Coon, D., & Bosland, P.W. (2021). Single nucleotide polymorphisms reveal genetic diversity in New Mexican chile peppers (Capsicum spp.). BMC Genomics, 17, 356. https://doi.org.10.1186/s12864-021-07662-7
- Maharijaya, A., Vosman, B., Pelgrom, K., Wahyuni, Y., de Vos, R.C., & Voorrips, R.E. (2019). Genetic variation in phytochemicals in leaves of pepper (Capsicum) in relation to thrips resistance. Arthropod-Plant Interactions, 13, 1–9. https://doi.org/10.1007/s11829-018-9628-7
- Meckelmann, S.W., Riegel, D.W., van Zonneveld, M.J., Ríos, L., Peña, K., Ugas, R., Quinonez, L., Mueller-Seitz, E., & Petz, M. (2013). Compositional Characterization of Native Peruvian Chilli Peppers (Capsicum spp.). Journal of Agriculture Food and Chemistry, 61, 2530–2537.
- Milad, S.I., Wahba, L.E., & Barakat, M.N. (2011). Identification of RAPD and ISSR markers associated with flag leaf senescence under water-stressed conditions in wheat (Triticum aestivum L.). Australian Journal of Crop Science, 5, 337–343. https://doi.org/10.3316/informit.279862523722050
- Naegele, R.P., Mitchell, J., & Hausbeck, M.K. (2016). Genetic diversity, population structure, and heritability of fruit traits in Capsicum annuum. Plos one, 11. https://doi.org/10.1371/journal.pone.0156969
- Nilkanta, H., Amom, T., Tikendra, L., Rahaman, H., & Nongdam, P. (2017). ISSR marker based population genetic study of Melocanna baccifera (Roxb.) Kurz: a commercially important bamboo of Manipur, North-East India. Scientifica, 9. https://doi.org/10.1155/2017/3757238
- Rai, V.P., Kumar, R., Kumar, S., Rai, A., Kumar, S., Singh, M., Singh, S.P., Rai, A.B., & Paliwal, R. (2013). Genetic diversity in Capsicum germplasm based on microsatellite and random amplified microsatellite polymorphism markers. Physiology and Molecular Biology of Plants, 19, 575–586. https://doi.org/10.1007/s12298-013-0185-3
- Sharmin, A., Hoque, E., Haque, M., & Khatun, F. (2018). Molecular Diversity Analysis of Some Chilli (Capsicum spp.) Genotypes Using SSR Markers. American Journal of Plant Sciences, 9, 82464. https://doi.org.10.4236/ajps.2018.93029
- Shiragaki, K., Yokoi, S., & Tezuka, T. (2020). Phylogenetic Analysis and Molecular Diversity of Capsicum Based on rDNAITS Region. Horticulturae, 6, 87. https://doi.org/10.3390/horticulturae6040087
- Swamy, K.R.M. (2023). Origin, distribution, taxonomy, botanical description, genetic diversity and breeding of capsicum (Capsicum annuum L.). International Journal of Development Research, 13, 61956-61977.
- White, T.J. et al. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols a guide to methods and applications. Academic Press (pp. 315–322).
- Zhao, F., Li, D., Tian, X., & Du, W. (2020). QTL mapping for the resistance to yellow rust race CYR34 in triticale. Chilean Journal of Agricultural Research, 80, 405–412. https://doi.org/10.4067/S0718-58392020000300405
Language: English
Page range: 55 - 60
Submitted on: Feb 3, 2026
Accepted on: Mar 17, 2026
Published on: May 18, 2026
Published by: Slovak University of Agriculture in Nitra
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
Related subjects:
© 2026 Jana Žiarovská, Lucia Urbanová, Silvia Farkasová, Eloy Fernandez Cusimamani, published by Slovak University of Agriculture in Nitra
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.