Chemical composition and biological effects of Citrus aurantium var. dulce essential oil

Vukić, Milena D.; Branković, Jovica; Ðukić, Maja B.

doi:10.2478/ahr-2023-0003

Abstract

Citrus aurantium var. dulce (sweet orange) belongs to one of the largest genera of the Rutaceae family. The species of this genus are consumed worldwide fresh or in form of beverages. They include well-known crops lemons, oranges, mandarins, grapefruits, and limes. Essential oils (EOs) obtained from this species have great economic value since they are mainly produced from the peel of the crops, which are considered waste during their industrial processing. Considering, the aim of this study was to evaluate the chemical composition of the essential oil (EO) obtained from the peel of Citrus aurantium var. dulce, as well as to assess the biological effects by the means of antioxidant and antibacterial activity. Chemical composition analysis performed using GC and GC/MS analysis revealed that this EO is a rich source of limonene presented in this sample in the amount of 93.86% of the total. Obtained results of antioxidant testing indicate better inhibition of ABTS^•+ (68.32 ±3.54%) compared to the DPPH^• (8.60 ±1.52%). Moreover, the results of the antimicrobial assessment using the disc diffusion method displayed almost no inhibition power of this essential oil towards G⁻ bacteria and yeast strains, while towards G⁺ bacterial strains weak inhibition was observed.

References

Adams, R. P. (2007). Identification of Essential Oil Components By Gas Chromatography/Mass Spectroscopy. Allured Publishing Corporation. Ed., 4.
Search in Google Scholar Back to article
Akarca, G., & Sevik, R. (2021). Biological Activities of Citrus limon L. and Citrus sinensis L. Peel Essential Oils. Journal of Essential Oil Bearing Plants, 24(6), 1415–1427. https://doi.org/10.1080/0972060X.2021.2022000
Search in Google Scholar Back to article
Aljaafari, M. N., AlAli, A. O., Baqais, L., Alqubaisy, M., AlAli, M., Molouki, A., Ong-Abdullah, J., Abushelaibi, A., Lai, K.-S., & Lim, S.-H. E. (2021). An Overview of the Potential Therapeutic Applications of Essential Oils. Molecules, 26(3), 628. https://doi.org/10.3390/molecules26030628
Search in Google Scholar Back to article
Baptista-Silva, S., Borges, S., Ramos, O. L., Pintado, M., & Sarmento, B. (2020). The progress of essential oils as potential therapeutic agents: a review. Journal of Essential Oil Research, 32(4), 279–295. https://doi.org/10.1080/10412905.2020.1746698
Search in Google Scholar Back to article
Bendahaa, H., Bouchalb, B., el Mounsia, I., Salhic, A., Berrabehd, M., el Bellaouib, M., & Mimounia, M. (2016). Chemical composition, antioxidant, antibacterial and antifungal activities of peel essential oils of Citrus aurantium grown in Eastern Morocco. Der Pharmacia Lettre, 8(4), 239–245.
Search in Google Scholar Back to article
Bora, H., Kamle, M., Mahato, D. K., Tiwari, P., & Kumar, P. (2020). Citrus Essential Oils (CEOs) and Their Applications in Food: An Overview. Plants, 9(3), 357. https://doi.org/10.3390/plants9030357
Search in Google Scholar Back to article
Bourgou, S., Rahali, F. Z., Ourghemmi, I., & Saïdani Tounsi, M. (2012). Changes of Peel Essential Oil Composition of Four Tunisian Citrus during Fruit Maturation. The Scientific World Journal, 2012, 1–10. https://doi.org/10.1100/2012/528593
Search in Google Scholar Back to article
Bousbia, N., Vian, M. A., Ferhat, M. A., Meklati, B. Y., & Chemat, F. (2009). A new process for extraction of essential oil from Citrus peels: Microwave hydrodiffusion and gravity. Journal of Food Engineering, 90(3), 409–413. https://doi.org/10.1016/j.jfoodeng.2008.06.034
Search in Google Scholar Back to article
Christaki, S., Moschakis, T., Kyriakoudi, A., Biliaderis, C. G., & Mourtzinos, I. (2021). Recent advances in plant essential oils and extracts: Delivery systems and potential uses as preservatives and antioxidants in cheese. Trends in Food Science & Technology, 116, 264–278. https://doi.org/10.1016/j.tifs.2021.07.029
Search in Google Scholar Back to article
de Araújo, J. S. F., de Souza, E. L., Oliveira, J. R., Gomes, A. C. A., Kotzebue, L. R. V., da Silva Agostini, D. L., de Oliveira, D. L. V., Mazzetto, S. E., da Silva, A. L., & Cavalcanti, M. T. (2020). Microencapsulation of sweet orange essential oil (Citrus aurantium var. dulcis) by liophylization using maltodextrin and maltodextrin/gelatin mixtures: Preparation, characterization, antimicrobial and antioxidant activities. International Journal of Biological Macromolecules, 143, 991–999. https://doi.org/10.1016/j.ijbiomac.2019.09.160
Search in Google Scholar Back to article
Fisher, K., & Phillips, C. A. (2006). The effect of lemon, orange and bergamot essential oils and their components on the survival of Campylobacter jejuni, Escherichia coli O157, Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus in vitro and in food systems. Journal of Applied Microbiology, 101(6), 1232–1240. https://doi.org/10.1111/j.1365-2672.2006.03035.x
Search in Google Scholar Back to article
Floegel, A., Kim, D.-O., Chung, S.-J., Koo, S. I., & Chun, O. K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis, 24(7), 1043–1048. https://doi.org/10.1016/j.jfca.2011.01.008
Search in Google Scholar Back to article
Friedman, M., Henika, P. R., & Mandrell, R. E. (2002). Bactericidal Activities of Plant Essential Oils and Some of Their Isolated Constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. Journal of Food Protection, 65(10), 1545–1560. https://doi.org/10.4315/0362-028X-65.10.1545
Search in Google Scholar Back to article
Galovičová, L., Čmiková, N., Vukovic, N., Vukic, M., Kowalczewski, P. Ł., Bakay, L., & Kačániová, M. (2022). Chemical Composition, Antioxidant, Antimicrobial, Antibiofilm and Anti-Insect Activities of Jasminum grandiflorum Essential Oil. Horticulturae, 8(10), 953. https://doi.org/10.3390/horticulturae8100953
Search in Google Scholar Back to article
Geraci, A., di Stefano, V., di Martino, E., Schillaci, D., & Schicchi, R. (2017). Essential oil components of orange peels and antimicrobial activity. Natural Product Research, 31(6), 653–659. https://doi.org/10.1080/14786419.2016.1219860
Search in Google Scholar Back to article
González-Mas, M. C., Rambla, J. L., López-Gresa, M. P., Blázquez, M. A., & Granell, A. (2019). Volatile Compounds in Citrus Essential Oils: A Comprehensive Review. Frontiers in Plant Science, 10. https://doi.org/10.3389/fpls.2019.00012
Search in Google Scholar Back to article
Mahato, N., Sharma, K., Koteswararao, R., Sinha, M., Baral, E., & Cho, M. H. (2019). Citrus essential oils: Extraction, authentication and application in food preservation. Critical Reviews in Food Science and Nutrition, 59(4), 611–625. https://doi.org/10.1080/10408398.2017.1384716
Search in Google Scholar Back to article
Palazzolo, E., Armando Laudicina, V., & Antonietta Germanà, M. (2013). Current and Potential Use of Citrus Essential Oils. Current Organic Chemistry, 17(24), 3042–3049. https://doi.org/10.2174/13852728113179990122
Search in Google Scholar Back to article
Popović-Djordjević, J., Cengiz, M., Ozer, M. S., & Sarikurkcu, C. (2019). Calamintha incana: Essential oil composition and biological activity. Industrial Crops and Products, 128, 162–166. https://doi.org/10.1016/j.indcrop.2018.11.003
Search in Google Scholar Back to article
Proestos, C., Lytoudi, K., Mavromelanidou, O., Zoumpoulakis, P., & Sinanoglou, V. (2013). Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils. Antioxidants, 2(1), 11–22. https://doi.org/10.3390/antiox2010011
Search in Google Scholar Back to article
Ruiz, B., & Flotats, X. (2014). Citrus essential oils and their influence on the anaerobic digestion process: An overview. Waste Management, 34(11), 2063–2079. https://doi.org/10.1016/j.wasman.2014.06.026
Search in Google Scholar Back to article
Singh, B., Singh, J. P., Kaur, A., & Yadav, M. P. (2021). Insights into the chemical composition and bioactivities of citrus peel essential oils. Food Research International, 143, 110231. https://doi.org/10.1016/j.foodres.2021.110231
Search in Google Scholar Back to article
Thielmann, J., & Muranyi, P. (2019). Review on the chemical composition of Litsea cubeba essential oils and the bioactivity of its major constituents citral and limonene. Journal of Essential Oil Research, 31(5), 361–378. https://doi.org/10.1080/10412905.2019.1611671
Search in Google Scholar Back to article
van den Dool, H., & Dec. Kratz, P. (1963). A generalization of the retention index system including linear temperature programmed gas – liquid partition chromatography. Journal of Chromatography A, 11, 463–471. https://doi.org/10.1016/S0021-9673(01)80947-X
Search in Google Scholar Back to article
Vieira, A. J., Beserra, F. P., Souza, M. C., Totti, B. M., & Rozza, A. L. (2018). Limonene: Aroma of innovation in health and disease. Chemico-Biological Interactions, 283, 97–106. https://doi.org/10.1016/j.cbi.2018.02.007
Search in Google Scholar Back to article

Chemical composition and biological effects of Citrus aurantium var. dulce essential oil

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