Effects of Gibberellic Acid and Low Temperature on Germination of Some Prunus Species Embryos (Without Cotyledons) Under Laboratory Conditions

Ghayyad, Mahmoud. A.

doi:10.2478/ahr-2018-0010

Abstract

Seeds of Prunus species do not germinate as a result of different mechanisms of dormancy such as physiological, physical and/or chemical ones. This study was carried out in order to determine the effects of three concentrations of Gibberellic acid (GA₃) 1, 3, and 5 mg.L⁻¹ and low temperature at 5 °C on germination and on the length of isolated embryos from cotyledons of almond, apricot, plum, peach, mahaleb and sweet cherry on top of filter paper under laboratory conditions. The highest germination percentage (96.67%) was at 1 mg.L⁻¹ GA₃ without cold treatment or 3 mg.L⁻¹ GA₃ + cold treatment in almond, the highest length of embryos (15.47 mm) was also in almond at 1 mg.L⁻¹ GA₃ without cold treatment. Embryos of mahaleb and sweet cherry germinated at low germination percentages of 31.16%, 33.33% respectively at 3 mg.L⁻¹ GA₃ + cold treatment. It was concluded that embryos of almond, apricot, plum and peach were germinated successfully on top of filter paper under laboratory conditions and GA₃ increased significantly the germination percentages of the isolated embryos either after cold treatment or without compared with controls. A strong positive correlation was found between seed germination without testa, embryos germination and final embryos length simultaneously.

References

ARBELOA, A. – DAORDEN, M.E. – GARCIA, E. – ANDREU, P. – MARIN, J.A. 2009. In vito culture of Myroplan (Prunus cerasifera Ehrh) embryos. In HortScience, 2009, no. 44, pp. 1672–1674.10.21273/HORTSCI.44.6.1672
Search in Google Scholar Back to article
GERCEKCIOGLU, R. – CEKIC, C. 1999. The effects of some treatments on germination of mahaleb (Prunus mahaleb L.) seeds. In Turkish J Agric Forestry, 1999, no. 23, pp. 145–150.
Search in Google Scholar Back to article
GENEVE, R.L. 1999. Seed dormancy in commercial vegetable and flower species. In Proc. Intl. Plant Prop. Soc., 1999, no. 49, pp. 248–254.
Search in Google Scholar Back to article
GHAYYAD, M. – KURBYSA, M. – NAPOLSY, G. 2010. Effect of endocarp removal, gibberelline, stratification and sulfuric acid on germination of Mahaleb (Prunus mahaleb L.) seeds. In American-Eurasian Journal of Agricultural and Environmental Science, 2010, no. 9, pp. 163–168 https://www.idosi.org/aejaes/jaes9(2)/10.pdf
Search in Google Scholar Back to article
HARTMANN, H.T. – KESTER, D.E. – DAVIES, F. JR. – GENEVE, R.L.1997. Plant Propagation Principles and Practices. 6^th ed., New Jersey : Prentice Hall, 1997.
Search in Google Scholar Back to article
HASSANEIN, A.M. – AZOOZ, M.M. 2003. Propagation of Citrus reticulata via in vitro seed germination and shoot cuttings. In Biol. Plant., 2003, no. 47, pp. 173–177.10.1023/B:BIOP.0000022247.46798.59
Search in Google Scholar Back to article
HORMAZA, J.I. 1999. Early selection in cherry combining RAPDs with embryo culture. In Sci. Hort., 1999, no. 79, pp. 121–126.10.1016/S0304-4238(98)00204-0
Search in Google Scholar Back to article
JOHNSON, G. – CHIRCO, E. 2003. Excised embryo tests of peach, apple and pear. Proceedings of the ISTA Forest Tree and Shrub Seed Committee Workshop Prague – Průhonice, Czech Republic, October 20–22. Forestry and Game Management Research Institute Jiloviště-Strnady, CR and Forestry Commission Research Agency, UK. http://www.vulhm.cz/sites/File/vydavatelska_cinnost/sborniky_a_dalsi_publikace/sbornik_ista.pdf
Search in Google Scholar Back to article
KAUR, R. – SHARMA, N. – KUMAR, K. – SHARMA, D.R. – SHARMA, S.D. 2006. In vitro germination of walnut (Juglans regia L.) embryos. In Scientia Horticulturae, 2006, no. 109, pp. 385–388. http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.845.1786&rep=rep1&type=pdf10.1016/j.scienta.2006.05.012
Search in Google Scholar Back to article
LEDBETTER, C.A. – PALMQUIST, D.A. – PETERSON, S.J. 1998. Germination and net in vitro growth of peach, almond, and peach-almond hybrid embryos in response to mannitol inclusion in the nutrient medium. In Euphytica, 1998, no. 103, pp. 243–250.10.1023/A:1018324624252
Search in Google Scholar Back to article
MILLER, A.R. – SCHEEREUS, J.C. – ERB, P.S. – CHANDLER, C.K. 1992. Enhanced strawberry seed germination through in vitro culture of cut achenes. In J. Amer. Soc. Hort. Sci., 1992, no. 117, pp. 313–316.10.21273/JASHS.117.2.313
Search in Google Scholar Back to article
NING, G.G. – BAI, S.P. – BAO, M.Z. – LIU, L. 2007. Factors affecting plantlet regeneration from in vitro cultured immature embryos and cotyledons of Prunus mume ‘Xue mei’. In In Vitro Cell. Dev. Biol. Plant, 2007, no. 43, pp. 225–230.10.1007/s11627-006-9006-5
Search in Google Scholar Back to article
PAYGHAMZADEH, K. – KAZEMITABAR, S.K. 2010a. The effects of BAP, IBA and genotypes on in vitro germination of immature walnut embryos. In International Journal of Plant Production, vol. 4, 2010, no. 4, pp. 309–322. http://ijpp.gau.ac.ir/article_714_fe51cf9369d57b06b13921ab106e59b0.pdf
Search in Google Scholar Back to article
PAYGHAMZADEH, K. – KAZEMITABAR, S.K. 2010b. In vitro germination of Pecan (Carya illinoinensis) embryo. In Biharean Biologist, 2010, no. 4, pp. 37–43. http://biozoojournals.ro/bihbiol/cont/v4n1/bb.041106.Kazemitabar.pdf
Search in Google Scholar Back to article
PIPINIS, E. – MILIOS, E. – MAVROKORDOPOULOU, O. – GKANATSIOU, C. – ASLANIDOU, M. – SMIRIS, P. 2012. Effect of pretreatments on seed germination of Prunus mahaleb L. In Not. Bot. Horti Agrobo., vol. 40, 2012, no. 2, pp. 183–189. http://www.notulaebotanicae.ro/index.php/nbha/article/viewFile/7887/702210.15835/nbha4027887
Search in Google Scholar Back to article
SAN, B. – YILDIRIM, A.N. 2009. Seed and in vitro embryo germination in aged almond. In Seed Sci. and Technol., 2009, no. 37, pp. 365–371. https://doi.org/10.15258/sst.2009.37.2.1010.15258/sst.2009.37.2.10
Search in Google Scholar Back to article
SAN, P. – YILDIRIM, A.N. – YILDIRIM, F. 2014. An In Vitro Germination Technique for Some Stone Fruit Species: the Embryo Isolated from Cotyledons Successfully Germinated without Cold Pre-treatment of Seeds. In Hortscience, vol. 49, 2014, no. 3, pp. 294–296. http://hortsci.ashspublications.org/content/49/3/294.full10.21273/HORTSCI.49.3.294
Search in Google Scholar Back to article
SCALTSOYIANNES, A. – TSOULPHA, P. – PANETSOS, K.P. – MOULALIS, D. 1997. Effect of genotype on micropropagation of walnut trees (Juglans regia L.). In Sil. Gen., 1997, no. 46, pp. 326–332.
Search in Google Scholar Back to article

Effects of Gibberellic Acid and Low Temperature on Germination of Some Prunus Species Embryos (Without Cotyledons) Under Laboratory Conditions

Abstract

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