Have a personal or library account? Click to login
EGF enema and EGFR monoclonal antibody injection alleviate the inflammatory bowel disease in AMO/DSS induced mice model Cover

EGF enema and EGFR monoclonal antibody injection alleviate the inflammatory bowel disease in AMO/DSS induced mice model

Postępy Higieny i Medycyny Doświadczalnej
Volume 79 (2025): Issue 1 (January 2025)
By: Xueyan Zheng and  Chengdang Wang  
Open Access
|Jun 2025

Figures & Tables

Figure 1.

The effect of EGFR mAb treatment combined with EGF enema in the IBD model in vivo. (A) Line-graph showing changes in body weight of mice (n=3 per group) in control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. Treatment time and cycles are mentioned. (B) Bar-graph showing changes in weight of colon tissues (grams) among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. Data is presented as mean ± SEM. * indicates comparison to the initial body weight (Week 0), and # denotes comparison to the body weight at Week 2. ns; non-significant. *P<0.05, **P<0.01, ***P<0.001, # P<0.05, ##P<0.01, ###P<0.001
The effect of EGFR mAb treatment combined with EGF enema in the IBD model in vivo. (A) Line-graph showing changes in body weight of mice (n=3 per group) in control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. Treatment time and cycles are mentioned. (B) Bar-graph showing changes in weight of colon tissues (grams) among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. Data is presented as mean ± SEM. * indicates comparison to the initial body weight (Week 0), and # denotes comparison to the body weight at Week 2. ns; non-significant. *P<0.05, **P<0.01, ***P<0.001, # P<0.05, ##P<0.01, ###P<0.001

Figure 2.

EGFR mAb treatment combined with EGF enema alleviates colonic inflammation in the IBD model in vivo. (A-D) Representative H&E staining images (100x) of colon tissue from control (A), AOM/DSS model (B), model with EGF enema (C) and model with EGF enema and EGFR mAb treatment (D)
EGFR mAb treatment combined with EGF enema alleviates colonic inflammation in the IBD model in vivo. (A-D) Representative H&E staining images (100x) of colon tissue from control (A), AOM/DSS model (B), model with EGF enema (C) and model with EGF enema and EGFR mAb treatment (D)

Figure 3.

EGFR mAb treatment combined with EGF enema reduces proliferation in the IBD model in vivo. (A) IHC images (100x) showing changes in expression of Ki67 and EGFR among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. (B) Bar-graph showing quantification of staining in (A). Data is presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001
EGFR mAb treatment combined with EGF enema reduces proliferation in the IBD model in vivo. (A) IHC images (100x) showing changes in expression of Ki67 and EGFR among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. (B) Bar-graph showing quantification of staining in (A). Data is presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001

Figure 4.

EGFR mAb treatment combined with EGF enema reduces oncogenic signaling in the IBD model in vivo. (A) Immunoblots showing changes in expression of p-AKT, p-ERK and EGFR among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. (B) Bar-graph showing quantification of changes in protein expression in (A). Data is presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001
EGFR mAb treatment combined with EGF enema reduces oncogenic signaling in the IBD model in vivo. (A) Immunoblots showing changes in expression of p-AKT, p-ERK and EGFR among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. (B) Bar-graph showing quantification of changes in protein expression in (A). Data is presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001
DOI: https://doi.org/10.2478/ahem-2025-0011 | Journal eISSN: 1732-2693
Journal RSS Feed
Language: English
Page range: 87 - 94
Submitted on: Sep 19, 2024
Accepted on: May 12, 2025
Published on: Jun 21, 2025
Published by: Hirszfeld Institute of Immunology and Experimental Therapy
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
inflammatory bowel disease,
AOM/DSS model,
EGF,
EGFR,
monoclonal antibody
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Medicine,
Basic medical science,
Immunology

© 2025 Xueyan Zheng, Chengdang Wang, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Previous articleVolume 79 (2025): Issue 1 (January 2025)Next article