Table 1.
The antiproliferative activity of tested extracts presented as the mean IC50 value ± standard deviation (SD). The extracts were dissolved in culture medium
| A-498 | HepG2 | PLC/PRF/5 | MDA-MB-231 | MV4-11 | BALB/3T3 | |
|---|---|---|---|---|---|---|
| IC50 (mean ± SD) [μg/mL] | ||||||
| Aristolochia baetica | 430.20±138.23 | 340.94±143.0 | 722.25±103.98 | 241.21±73.38 | 277.08±87.26 | 387.69±142.75 |
| Cistus lanifedus | 528.65±277.15 | 759.24±200.31 | n.a. | 403.77±157.14 | n.t. | 604.93±315.09 |
| Crataegus Monogyna (Leaves) | n.a. | n.a. | n.a. | 598.15±45.96 | n.t. | n.a. |
| Crataegus Monogyna (Fruit) | n.a. | n.a. | n.a. | 84.99±11.11* | n.t. | n.a. |
| Erica arborea | 347.27±106.93 | 481.40±148.33 | n.a. | 450.48±158.56 | n.a. | 288.68±11.00 |
| Rhamnus alaternus | n.a. | 729.25±205.32 | n.a. | n.a. | n.t. | n.a. |
| Lavendula dentata | 375.60±87.05 | 317.38±25.75 | 354.15±13.99 | 277.91±24.73 | 684.34±192.55 | 226.85±25.63 |
| Cisplatin | 2.64+±0.78 | 1.32±0.94 | 1.64±0.29 | 3.11±0.48 | 0.39±0.047 | 0.50±0.32 |
Figure 1.
Caspase-3/7 activity after 72 h of HepG2 cells treatment with extracts at 200 µg/ml concentration. Concentrations of cytostatics: Cisplatin – 1 µg/ml, Doxorubicin – 10 µM, and Camptothecin – 0,5 µg/ml. Doxorubicin and Camptothecin were added 24 h before Caspase-3/7 assay. The normality of the data distribution was performed using the Shapiro-Wilk test. One-way ANOVA tests with Dunnett’s multiple comparisons test were performed. **** - p< 0,0001 vs. ctrl. The number of observations for ctrl and Aristolochia baetica – 7, Lavendula dentata – 6, Cisplatin – 5, Doxorubicin, and Camptothecin – 3.
Table 2.
Fold mean relative caspase-3/7 activity in comparison to the untreated control ± SD in HepG2 cells
| Mean relative caspase-3/7 activity ± SD | |
|---|---|
| Aristolochia baetica (200μg/ml) | 0.680±0.134 |
| Lavendula dentata (200μg/ml) | 0.795±0.120 |
| Cisplatin (1μg/ml) | 1.425±0.067 |
| Doxorubicin (10μM) | 4.890±1.096 |
| Camptothecin (0,5μg/ml) | 8.975±0.028 |
Figure 2.
The cell cycle analysis for extracts at 1000 µg/ml after 72 hours of treatment of MV4-11 cells. Concentrations of cisplatin – 0.4 µg/ml. The number of observations for ctrl – 5, Aristolochia baetica and Lavendula dentata – 4, Cisplatin – 6. Two-way ANOVA and Dunnett’s multiple comparisons test was used for cell cycle analysis. *- p < 0.05, **- p < 0.01, ***- p < 0.001, ****-p < 0.0001 vs. ctrl
Figure 3.
The cell cycle analysis for extracts at 100 µg/ml after 72 hours of treatment of MV4-11 cells. Concentrations of cisplatin – 0.4 µg/ml. The number of observations for ctrl – 5, Aristolochia baetica and Lavendula dentata – 4, Cisplatin – 6. Two-way ANOVA and Dunnett’s multiple comparisons test was used for cell cycle analysis. *- p < 0.05, **- p < 0.01, ****-p < 0.0001 vs. ctrl.