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Evaluation of antitumoral effect of mistletoe fruit extract on Ehrlich ascites tumor cells with muse cell analyzer and argyrophilic nucleolar organizer region staining method Cover

Evaluation of antitumoral effect of mistletoe fruit extract on Ehrlich ascites tumor cells with muse cell analyzer and argyrophilic nucleolar organizer region staining method

Postępy Higieny i Medycyny Doświadczalnej
Volume 76 (2022): Issue 1 (January 2022)
By: Şükrü Ateş,  Harun Ülger,  Seher Yilmaz,  Gökçe Şeker Karatoprak,  Özge Al,  Sümeyye Uçar,  Mustafa Taştan,  Adem Tokpinar,  Şerife Alpa and  Ammad Ahmad Farooqi  
Open Access
|Jul 2022

Figures & Tables

Fig. 1

Experimental processes in this study
Experimental processes in this study

Fig. 2

The raw graphics taken from the Muse® Cell Analyzer. A: Muse® Annexin V and Dead Cell Assay, B: Muse® Cell Cycle Assay, C: Muse® Mitopotential Assay
The raw graphics taken from the Muse® Cell Analyzer. A: Muse® Annexin V and Dead Cell Assay, B: Muse® Cell Cycle Assay, C: Muse® Mitopotential Assay

Fig. 3

Data of the annexin V & dead cell assay at the end of 24, 48, and 72 hours of incubation. p<0.05 was considered statistically significant. Data are expressed as mean ± SEM (standard error of mean). There is no statistically significant difference between the groups containing the same letter (p> 0.05). A: Live cell count (%), B: Early apoptotic cell count (%), C: Late apoptotic cell count (%), D: Total apoptotic cell count (%). 5-FU: 5-fluorouracil; 100, 200, 400, 800 μg/ml: Mistletoe groups
Data of the annexin V & dead cell assay at the end of 24, 48, and 72 hours of incubation. p<0.05 was considered statistically significant. Data are expressed as mean ± SEM (standard error of mean). There is no statistically significant difference between the groups containing the same letter (p> 0.05). A: Live cell count (%), B: Early apoptotic cell count (%), C: Late apoptotic cell count (%), D: Total apoptotic cell count (%). 5-FU: 5-fluorouracil; 100, 200, 400, 800 μg/ml: Mistletoe groups

Fig. 4

Data of the cell cycle assay at the end of 24, 48, and 72 hours of incubation. p <0.05 was considered statistically significant. Data are expressed as mean ± SEM. There is no statistically significant difference between groups containing the same letter (p> 0.05). A: Cell cycle distribution-G0 / G1 stage (%), B: Cell cycle distribution-S stage (%), C: Cell cycle distribution-G2 / M stage (%). 5-FU: 5-fluorouracil; 100, 200, 400, 800 μg/ml: Mistletoe groups
Data of the cell cycle assay at the end of 24, 48, and 72 hours of incubation. p <0.05 was considered statistically significant. Data are expressed as mean ± SEM. There is no statistically significant difference between groups containing the same letter (p> 0.05). A: Cell cycle distribution-G0 / G1 stage (%), B: Cell cycle distribution-S stage (%), C: Cell cycle distribution-G2 / M stage (%). 5-FU: 5-fluorouracil; 100, 200, 400, 800 μg/ml: Mistletoe groups

Fig. 5

Data of the mitopotential assay at the end of 24, 48, and 72 hours of incubation. p <0.05 was considered statistically significant. Data are expressed as mean ± SEM. There is no statistically significant difference between the groups containing the same letter (p> 0.05). A: Live depolarized cell count (%), B: Dead depolarized cell count (%), C: Total depolarized cell count (%). 5-FU: 5-fluorouracil; 100, 200, 400, 800 μg/ml: Mistletoe groups
Data of the mitopotential assay at the end of 24, 48, and 72 hours of incubation. p <0.05 was considered statistically significant. Data are expressed as mean ± SEM. There is no statistically significant difference between the groups containing the same letter (p> 0.05). A: Live depolarized cell count (%), B: Dead depolarized cell count (%), C: Total depolarized cell count (%). 5-FU: 5-fluorouracil; 100, 200, 400, 800 μg/ml: Mistletoe groups

Fig. 6

Image examples of the AgNOR staining cells. A. Control group, B. 5-FU group, C. 100 μg / ml mistletoe group, D. 200 μg/ml mistletoe group, E. 400 μg / ml mistletoe group, F. 800 μg/ml mistletoe group
Image examples of the AgNOR staining cells. A. Control group, B. 5-FU group, C. 100 μg / ml mistletoe group, D. 200 μg/ml mistletoe group, E. 400 μg / ml mistletoe group, F. 800 μg/ml mistletoe group

Mean AgNOR number after 24, 48, and 72 hours of incubation

Hours/GroupsControl5-FU100 μg/ml200 μg/ml400 μg/ml800 μg/mlp
24 hours3.54±1.26a2.82±1.06b2.98±1.16ab1.74±0.77c1.74±1.00c1.80±1.19c<0.001
48 hours3.68±1.55a3.16±1.07a2.40±0.78b2.12±1.25b1.96±0.75b1.28±0.53c<0.001
72 hours1.52±0.64a2.20±1.08bc2.26±1.24c2.20±1.01bc1.68±0.76ab1.74±0.85abc<0.001

Total phenolic and flavonoid contents of mistletoe fruit

Total phenol (mgGAE/gextract)Total flavonoid (mgCAE/gextract)
Mistletoe fruit6.24±2.264.82±1.36

TAA / NA value at the end of 24, 48, and 72 hours of incubation

Hours/GroupsControl5-FU100 μg/ml200 μg/ml400 μg/ml800 μg/mlp
24 hours0.11±0.04ab0.14±0.04b0.10±0.03ac0.13±0.06ab0.08±0.04c0.10±0.05ac<0.001
48 hours0.27±0.15a0.23±0.05a0.17±0.08b0.13±0.12bc0.09±0.05c0.09±0.07c<0.001
72 hours0.10±0.04ac0.12±0.04ab0.13±0.05b0.10±0.06ac0.08±0.04c0.11±0.04bc<0.001
DOI: https://doi.org/10.2478/ahem-2022-0014 | Journal eISSN: 1732-2693
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Language: English
Page range: 209 - 219
Submitted on: Mar 5, 2021
|
Accepted on: Jan 4, 2022
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Published on: Jul 11, 2022
Published by: Hirszfeld Institute of Immunology and Experimental Therapy
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
mistletoe,
Ehrlich ascites tumor,
apoptosis,
cell proliferation,
AgNORs
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Medicine,
Basic medical science,
Immunology

© 2022 Şükrü Ateş, Harun Ülger, Seher Yilmaz, Gökçe Şeker Karatoprak, Özge Al, Sümeyye Uçar, Mustafa Taştan, Adem Tokpinar, Şerife Alpa, Ammad Ahmad Farooqi, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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