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Effect of amygdalin on MCF-7, MDA-MB-231 and T-47D breast cancer cells in the in vitro study Cover

Effect of amygdalin on MCF-7, MDA-MB-231 and T-47D breast cancer cells in the in vitro study

Postępy Higieny i Medycyny Doświadczalnej
Volume 76 (2022): Issue 1 (January 2022)
By: Aleksandra Zielińska,  Justyna Płonka-Czerw,  Agnieszka Nowak and  Dariusz Kuśmierz  
Open Access
|Apr 2022

Figures & Tables

Fig. 1

Comparison of the chemical structure of amygdalin and laetrile.
Comparison of the chemical structure of amygdalin and laetrile.

Fig. 2

Comparison of the confluence degree between the cell cultures treated with amygdalin at a concentration of 50 mg/ml and the control cultures – not treated with amygdalin. The microscopic image [100x] was collected after 48 hours of cell cultures incubation. Scale bar = 100 μm.
Comparison of the confluence degree between the cell cultures treated with amygdalin at a concentration of 50 mg/ml and the control cultures – not treated with amygdalin. The microscopic image [100x] was collected after 48 hours of cell cultures incubation. Scale bar = 100 μm.

Fig. 3

The effect of different doses of amygdalin (0 – 100 mg/ml) on the percentage of attached, viable cells (CVDE test), on the metabolic activity of cells (WST-1 test) and on the percentage of dying cells (LDH test) in the HFF-1, T-47D, MDA-MB-231, and MCF-7 in vitro cultures. Statistical significance means p <0.05 (*).
The effect of different doses of amygdalin (0 – 100 mg/ml) on the percentage of attached, viable cells (CVDE test), on the metabolic activity of cells (WST-1 test) and on the percentage of dying cells (LDH test) in the HFF-1, T-47D, MDA-MB-231, and MCF-7 in vitro cultures. Statistical significance means p <0.05 (*).

Fig. 4

The effect of amygdalin at a concentration of 50 mg/ml on the BAX, BCL2, and caspase-3 mRNA level in human breast cancer cells (T-47D, MDA-MB-231, MCF-7) and normal dermal fibroblasts in vitro compared to control (cell cultures not treated with amygdalin). Statistical significance means p <0.05 (*).
The effect of amygdalin at a concentration of 50 mg/ml on the BAX, BCL2, and caspase-3 mRNA level in human breast cancer cells (T-47D, MDA-MB-231, MCF-7) and normal dermal fibroblasts in vitro compared to control (cell cultures not treated with amygdalin). Statistical significance means p <0.05 (*).

Fig. 5

The BAX/BCL2 ratio (BAX mRNA copy number/BCL-2 mRNA copy number) in culture cells treated with amygdalin (at concentration of 50 mf/ml) compared to control (culture cells not treated with amygdalin). Statistical significance means p <0.05 (*).
The BAX/BCL2 ratio (BAX mRNA copy number/BCL-2 mRNA copy number) in culture cells treated with amygdalin (at concentration of 50 mf/ml) compared to control (culture cells not treated with amygdalin). Statistical significance means p <0.05 (*).

Human cell lines used in the study_

Cells characteristicsCel lines*
T-47DMCF-7MDA-MB-231HFF-1
MorphologyEphithelialEphithelialEphithelialFibroblasts
TissueMammary gland/derived from metastatic site: pleural effusionMammary gland/derived from metastatic site: pleural effusionMammary gland/derived from metastatic site: pleural effusionSkin: foreskin
DiseaseDuctal carcinoma/estrogen-dependentAdenocarcinoma/estrogen-dependentAdenocarcinoma/estrogen-independentNormal
Donor54 years adult/female69 years adult/female51 years adult/femaleNewborn/male

Nucleotide sequences of the primers used in the RT-qPCR reaction_

Primer nameNucleotide sequenceTranscript detectedGenBank database access number
KAS3F5′-GGCCTGCCGTGGTACAGAACTGG-3′CASP3 mRNANM032991
KAS3R5′-AGCGACTGGATGAACCAGGAGCCA-3′
BAXF5′-CCTGTGCACCAAGGTGCCGGAACT-3′BAX mRNAL22474
BAXR5′-CCACCCTGGTCTTGGATCCAGCCC-3′
BCL-2F5′-TTGTGGCCTTCTTTGAGTTCGGTG-3′BCL2 mRNAM14745
BCL-2R5′-GGTGCCGGTTCAGGTACATCAGTCA-3′
18SrRNAF5′-CAGTTATGGTTCCTTTGGTCGCTC-3′18S rRNAM10098
18SrRNAR5′-GTTGATAGGGCAGACGTTCGAATG-3′
DOI: https://doi.org/10.2478/ahem-2022-0013 | Journal eISSN: 1732-2693
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Language: English
Page range: 132 - 142
Submitted on: Mar 30, 2021
Accepted on: Jan 12, 2022
Published on: Apr 25, 2022
Published by: Hirszfeld Institute of Immunology and Experimental Therapy
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
breast cancer,
amygdalin,
cell culture,
T-47D,
MDA-MB-231,
MCF-7,
HFF-1,
BAX,
BCL-2,
caspase 3
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Medicine,
Basic medical science,
Immunology

© 2022 Aleksandra Zielińska, Justyna Płonka-Czerw, Agnieszka Nowak, Dariusz Kuśmierz, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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