Have a personal or library account? Click to login
Assessment of a multiplex RT-PCR for Simultaneous, Rapid Screening of Common Viral Infections of Central Nervous System: A Prospective Study for Enteroviruses and Herpesviruses Cover

Assessment of a multiplex RT-PCR for Simultaneous, Rapid Screening of Common Viral Infections of Central Nervous System: A Prospective Study for Enteroviruses and Herpesviruses

Postępy Higieny i Medycyny Doświadczalnej
Volume 76 (2022): Issue 1 (January 2022)
By: Maryam Khalili,  Hamid Rahimi Hajiabadi,  Mojtaba Akbari,  Rana Saleh,  Bahram Nasr Esfahani and  Sharareh Moghim  
Open Access
|Apr 2022

Figures & Tables

Fig. 1

The multiplex PCR assay’s specificity was examined using PCR mixes containing all two-primer pairs and a single template (cDNA/DNA) from the positive controls. Lane 1: standard 100-bp DNA ladder marker. Lane 2: the mixture of enterovirus (440 bp) and HSV (292 bp) positive controls. Lane 3: enterovirus (440 bp), Lane 4: HSV (292 bp), Lane 5: negative control (E. coli)
The multiplex PCR assay’s specificity was examined using PCR mixes containing all two-primer pairs and a single template (cDNA/DNA) from the positive controls. Lane 1: standard 100-bp DNA ladder marker. Lane 2: the mixture of enterovirus (440 bp) and HSV (292 bp) positive controls. Lane 3: enterovirus (440 bp), Lane 4: HSV (292 bp), Lane 5: negative control (E. coli)

Fig. 2

Comparison of the sensitivity between multiplex PCR and monoplex PCR for the 10-fold serial dilutions of positive controls. (A): HSV (292 bp) (B): Enteroviruses (440 bp)
Comparison of the sensitivity between multiplex PCR and monoplex PCR for the 10-fold serial dilutions of positive controls. (A): HSV (292 bp) (B): Enteroviruses (440 bp)
DOI: https://doi.org/10.2478/ahem-2022-0011 | Journal eISSN: 1732-2693
Journal RSS Feed
Language: English
Page range: 91 - 96
Submitted on: May 25, 2021
Accepted on: Oct 29, 2021
Published on: Apr 2, 2022
Published by: Hirszfeld Institute of Immunology and Experimental Therapy
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
multiplex PCR,
central nervous system disease,
meningitis,
children,
enteroviruses,
herpes simplex virus-1/2
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Medicine,
Basic medical science,
Immunology

© 2022 Maryam Khalili, Hamid Rahimi Hajiabadi, Mojtaba Akbari, Rana Saleh, Bahram Nasr Esfahani, Sharareh Moghim, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Previous articleVolume 76 (2022): Issue 1 (January 2022)Next article