Fig. 1
Fig. 2
Relationships between the patient group and the control group regarding smoking, alcohol consumption, and gender
| Variables value | Controls (%) | Patients (%) | OR (95%CI) | P value |
|---|---|---|---|---|
| Tobacco | ||||
| (N=220) | ||||
| Non-smokers | 150 (75.0) | 31 (14.1) | Reference | |
| Smokers | 50 (25.0) | 189 (85.9) | 18.29 (11.13–30.06) | <0.0001 |
| (N=439) | ||||
| Non-smokers | 150 (75.0) | 124 (28.2) | Reference | |
| Smokers | 50 (25.0) | 315 (71.8) | 7.62 (5.20–11.16) | <0.0001 |
| Alcohol consumption | ||||
| (N=220) | ||||
| No | 101 (50.5) | 70 (31.8) | Reference | |
| Yes | 99 (49.5) | 150 (68.2) | 2.19 (1.47–3.25) | <0.0001 |
| (N=439) | ||||
| No | 101 (50.5) | 149 (33.9) | Reference | |
| Yes | 99 (49.5) | 290 (66.1) | 1.98 (1.41–2.79) | <0.0001 |
| Gender | ||||
| (N=220) | ||||
| Female | 100 (50.0) | 52 (23.6) | Reference | |
| Male | 100 (50.0) | 168 (76.4) | 3.23 (2.13–4.90) | <0.0001 |
| (N=439) | ||||
| Female | 100 (50.0) | 124 (28.2) | Reference | |
| Male | 100 (50.0) | 315 (71.8) | 2.54 (1.80–3.59) | <0.0001 |
Genotype and allele frequencies of MTHFD1 c_1958G>A and TCN2 c_776G>C polymorphisms in oral cavity cancer patients and controls
| Genotypes | Controls (%) | Patients (%) | OR (95% CI) | P value |
|---|---|---|---|---|
| MTHFD1c.1958G>A | ||||
| GG | 53 (26.5) | 55 (12.5) | 1.00 | |
| GA | 110 (55) | 332 (75.6) | 2.91 (1.88–4.49) | <0.0001 |
| AA | 37 (18.5) | 52 (11.9) | 1.35 (0.77–2.38) | 0.29 |
| G | 216 (54) | 442 (49) | 1.00 | |
| A | 184 (46) | 436 (51) | 1.16 (0.91–1.47) | 0.23 |
| TCN2 c.776G>C | ||||
| GG | 34 (17) | 76 (34.5) | 1.00 | |
| CG | 71 (35.5) | 107 (48.7) | 0.67 (0.41–1.12) | 0.13 |
| CC | 95 (47.5) | 37 (16.8) | 0.17 (0.10–0.30) | <0.0001 |
| G | 139 (35) | 259 (59) | 1.00 | |
| C | 261 (65) | 181 (41) | 0.76 (0.58–1.01) | 0.06 |
Detailed characterization of MTHFD1 and TCN2 gene polymorphisms
| Gene | The encoded protein | Number of polymorphism | Location | Function of polymorphism1 | Allele | MAF |
|---|---|---|---|---|---|---|
| MTHFD1 | Methylene-tetrahydrofolate-dehydrogenase | rs2236225 | 14: 63978598 | Arg653Gln | G/A | 0,46 |
| TCN2 | Transcobalamin 2 | rs1801198 | 22: 29341610 | Arg259Pro | G/C | 0,45 |
The primers, length of PCR products, and restriction enzymes
| Polymorphism | Primers | Product length | Restriction enzyme |
|---|---|---|---|
| MTHFD1 c.1958G>A | Sense | 331 bp | MspI |
| TCN2 c.776G>C | Sense | 568 bp | ScrFI |
Odds ratio of oral cavity cancer related to MTHFD1 and TCN2 genotypes according to tobacco use, alcohol consumption and gender in patients with head and neck cancer
| Risk factor | OR (95%CI) - P value | |
|---|---|---|
| MTHFD1c.1958G>A * | TCN2 c.776G>C ** | |
| Tobacco | ||
| Non-smokers | 2.10 (1.09–4.06) P=0.03 | 0.72 (0.29–1.77) P=0.63 |
| Smokers | 2.60 (1.29–5.23) P=0.01 | 0.15 (0.05–0.45) P<0.0001 |
| Alcohol consumption | ||
| No | 1.23 (0.60–2.51) P=0.35 | 0.53 (0.27–1.01) P=0.08 |
| Yes | 1.01 (0.57–1.81) P=1 | 0.26 (0.13–0.52) P<0.0001 |
| Gender | ||
| Female | 1.77 (0.93–3.39) P=0.1 | 0.48 (0.22–1.04) P=0.07 |
| Male | 2.28 (1.32–3.96) P=0.003 | 0.33 (0.17–0.61) P=0.003 |