Optimizing Micropropagation of Apple (Malus × Domestica Borkh) and in Vitro Root Induction By Piriformospora indica

Masoudi, Samira; Kermani, Maryam Jafarkhani; Soleimani, Ali; Hajnajari, Hassan; Alidadi, Amin; Hosseini, Zahra Saadat

doi:10.2478/agri-2020-0013

Abstract

The inoculation of plant species with mycorrhiza fungus Piriformospora indica results in enhancement of growth, increase in yield, and induction of resistance to biotic and abiotic diseases through improvement of the root system. The aim of the present study was to optimize in vitro propagation protocol for three indigenous apples (Malus × domestica) cultivars (ꞌGolbaharꞌ, ꞌSharbatiꞌ, ꞌSoltani Shabestariꞌ) and one commercial cultivar (ꞌGolden Deliciousꞌ). Furthermore, the efficiency of P. indica at rooting stage was investigated on three cultivars (ꞌSharbatiꞌ, ꞌSoltani Shabestariꞌ, ꞌGolden Deliciousꞌ). Establishment and proliferation stages were optimized by collecting explants at different seasons and comparing different culture media respectively. Rooting optimization included six treatments containing different concentrations of auxins in the presence or absence of P. indica. Results showed that at the establishment stage, a maximum percent of survival was observed in explants collected in spring. At the proliferation stage, different media had a divergent effects on distinct cultivars. Although all cultivars reacted favourable to micropropagation in MS (Murashige & Skoog 1962) basal medium, the presence or absence of cytokinin 2ip (N₆-(2-Isopentenyl) adenine) in the culture media showed significant and incremental improvements in growth indices. In all cultivars highest rooting percent, root length, root thickness, and the number of roots/explant was observed in MS media containing auxins for three weeks followed by a treatment of MS medium containing P. indica for another three weeks. Plantlets treated with P. indica, grow stronger and healthier at the acclimation stage compared to the ones that excluded P. indica.

References

ARAB, M.M. ‒ YADOLLAHI, A. ‒ SHOJAEIYAN, A. ‒ SHOKRI, S. ‒ GHOJAH, S.M. 2014. Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G × N15 (hybrid of almond × peach) vegetative rootstock. In Journal of Genetic Engineering and Biotechnology, vol. 12, no. 2, pp. 81 ‒ 87.10.1016/j.jgeb.2014.10.001
Search in Google Scholar Back to article
BATELJA LODETA, K. ‒ VUJEVIĆ, B. ‒ ŽANETIĆ, M. ‒ GUGIĆ, J. ‒ OČIĆ, V. ‒ ŠAKIĆ BOBIĆ, B. ‒ BENČIĆ, Đ. ‒ BOŠNJAK MIHOVILOVIĆ, A. ‒ HABUŠ JERČIĆ, I. ‒ ČMELIK, Z. ‒ KEREŠA. S. 2019. Evaluation of morphological and chemical characteristics and micropropagation of traditionally grown domesticated apple varieties in Croatia. In Journal of Central European Agriculture, vol. 20, no. 1, pp. 274 ‒ 291.10.5513/JCEA01/20.1.2305
Search in Google Scholar Back to article
BHATTACHARYA, A. ‒ SINGH, R. 2018. Optimization of micropropagation from nodal segments of apple (Malus × domestica. Borkh) cultivars golden delicious and red Fuji. In Current Journal of Applied Science and Technology, vol. 31, no. 3, pp. 1 ‒ 9.10.9734/CJAST/2018/45902
Search in Google Scholar Back to article
DONG, S. ‒ TIAN, Z. ‒ CHEN, P.J. ‒ SENTHIL KUMAR, R. ‒ SHEN, CH. ‒ CAI, D. ‒ YEH, K.W. 2013. The maturation zone is an important target of Piriformospora indica in Chinese cabbage roots. In Journal of Experimental Botany, vol. 64, no. 14, pp. 4529 ‒ 4540.10.1093/jxb/ert265
Search in Google Scholar Back to article
DRUEGE, U. ‒ BALTRUSCHAT, H. ‒ FRANKEN, P. 2007. Piriformospora indica promotes adventitious root formation in cuttings. In Scientia Horticulturae, vol. 112, no. 4, pp. 422 ‒ 426.10.1016/j.scienta.2007.01.018
Search in Google Scholar Back to article
FAO. 2016. Available on URL; http://www.Fao.Org.
Search in Google Scholar Back to article
FRANKEN, P. 2012. The plant strengthening root endophyte Piriformospora indica: potential application and the biology behind. In Applied Microbiology and Biotechnology, vol. 96, no. 6, pp. 1455 ‒ 1464.10.1007/s00253-012-4506-1
Search in Google Scholar Back to article
GENG, F. ‒ MORAN, R. ‒ DAY, M. ‒ HALTEMAN, W. ‒ ZHANG, D. 2016. Increasing in vitro shoot elongation and proliferation of ‘G. 30’and ‘G. 41’apple by chilling explants and plant growth regulators. In HortScience, vol. 51, no. 7, pp. 899 ‒ 904.10.21273/HORTSCI.51.7.899
Search in Google Scholar Back to article
HILBERT, M. ‒ VOLL, L.M. ‒ DING, Y. ‒ HOFMANN, J. ‒ SHARMA, M. ‒ ZUCCARO, A. 2012. Indole derivative production by the root endophyte Piriformospora indica is not required for growth promotion but for biotrophic colonization of barley roots. In New Phytologist, vol. 196, no. 2, pp. 520 ‒ 534.10.1111/j.1469-8137.2012.04275.x
Search in Google Scholar Back to article
HOHTOLA, A. 1988. Seasonal changes in explant viability and contamination of tissue cultures from mature Scots pine. In Plant Cell, Tissue and Organ Culture, vol. 15, no. 3, pp. 211 ‒ 222.10.1007/BF00033645
Search in Google Scholar Back to article
HUA, M.D.S. ‒ KUMAR, R.S. ‒ SHYUR, L.F. ‒ CHENG, Y.B. ‒ TIAN, Z. ‒ OELMÜLLER, R. ‒ YEH, K.W. 2017. Metabolomic compounds identified in Piriformospora indica- colonized Chinese cabbage roots delineate symbiotic functions of the interaction. In Scientific Reports, vol. 7, no. 1, pp. 9291.10.1038/s41598-017-08715-2
Search in Google Scholar Back to article
JAFARKHANI KERMANI, M. – HOSSEINI, Z.S. – HABASHI, A.A. 2009. A refined tissue culture medium for in vitro proliferation of apple rootstocks. In Acta Horticulturae, no. 829, pp. 313 – 318. DOI: 10.17660/ActaHortic.2009.829.48.10.17660/ActaHortic.2009.829.48
Search in Google Scholar Back to article
KAUR, A. ‒ SINGH, D. ‒ GUPTA, N. ‒ KUMAR, A. 2018. In vitro propagation of important rootstocks of apple for rapid cloning and improvement. In Biotechnologies of Crop Improvement, vol. 1, pp. 215 – 24110.1007/978-3-319-78283-6_6
Search in Google Scholar Back to article
LAHRMANN, U. ‒ ZUCCARO, A. 2012. Opprimo ergo sumevasion and suppression in the root endophytic fungus Piriformospora indica. In Molecular Plant-microbe Interactions, vol. 25, no. 6, pp. 727 – 737.10.1094/MPMI-11-11-0291
Search in Google Scholar Back to article
LI, D. ‒ MENSAH, R.A. ‒ LIU, F. ‒ TIAN, N. ‒ QI, Q. ‒ YEH, K. ‒ LAI, Z. 2019. Effects of Piriformospora indica on rooting and growth of tissue-cultured banana (Musa acuminata cv. Tianbaojiao) seedlings. In Scientia Horticulturae, vol. 257, pp. 108649.10.1016/j.scienta.2019.108649
Search in Google Scholar Back to article
LIN, H.F. – XIONG, J. – ZHOU, H.M. – CHEN, C.M. – LIN, F.Z. – XU, X.M. – YEH, K.W. 2019. Growth promotion and disease resistance induced in Anthurium colonized by the beneficial root endophyte Piriformospora indica. In BMC Plant Biology, vol. 19, no. 1, pp. 40.10.1186/s12870-019-1649-6
Search in Google Scholar Back to article
LIU, H. – SENTHILKUMAR, R. – MAM G. – ZOU, Q. – ZHU, K. – SHEN, X. – YEH, K.W. 2019. Piriformospora indica-induced phytohormone changes and root colonization strategies are highly host-specific. In Plant Signaling and Behavior, vol. 14, no. 9, pp. 1 – 13. DOI: 10.1080/15592324.2019.1632688.10.1080/15592324.2019.1632688676827531230564
Search in Google Scholar Back to article
MONFORT, L.E.F. – BERTOLUCCI, S.K.V. – LIMA, A.F. – DE CARVALHO, A.A. – MOHAMMED, A. – BLANK, A.F. – PINTO, J.E.B.P. 2018. Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum. In Industrial Crops and Products, vol. 116, pp. 231 – 239.10.1016/j.indcrop.2018.02.075
Search in Google Scholar Back to article
MOZAFFARIAN, V.A. 1996. Dictionary of Iranian plant names (Latin, English, Persian). Farhang Moaser Publlishers. ISBN 964-5545-40-4.
Search in Google Scholar Back to article
OBERSCHELP, G.P.J. – GONÇALVES, A.N. 2016. Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden. In In vitro Cellular and Developmental Biology-Plant, vol. 52, no. 1, pp. 28 – 37.10.1007/s11627-015-9740-7
Search in Google Scholar Back to article
SCHALLER, G.E. – BISHOPP, A. – KIEBER, J.J. 2015. The yin-yang of hormones: cytokinin and auxin interactions in plant development. In The Plant Cell, vol. 27, no. 1, pp. 44 – 63.10.1105/tpc.114.133595
Search in Google Scholar Back to article
SHAH, S. – THAPA, B.B. – CHAND, K. – PRADHAN, S. – SINGH, A. – VARMA, A. – PANT, B. 2019. Piriformospora indica promotes the growth of the in-vitro-raised Cymbidium aloifolium plantlet and their acclimatization. In Plant Signaling and Behavior, vol. 14, no. 6, pp. 1596716.10.1080/15592324.2019.1596716
Search in Google Scholar Back to article
SHAKYA, M. – GOTTEL, N. – CASTRO, H. – YANG, Z.K. – GUNTER, L. – LABBE, J. – PODAR, M. 2013. A multifactor analysis of fungal and bacterial community structure in the root microbiome of mature Populus deltoides trees. In PLoS One, vol. 8, no. 10, pp. e76382.10.1371/journal.pone.0076382
Search in Google Scholar Back to article
SIRRENBERG, A. – GOBEL, C. – GROND, S. – CZEMPINSKI, N. – RATZINGER, A. – KARLOVSKY, P. – SANTO S, P. – FEUSSNER, I. – PAWLOWSKI, K. 2007. Piriformospora indica affects plant growth by auxin production. In Physiologia Plantarum, vol. 131, no. 4, pp. 581 – 589.10.1111/j.1399-3054.2007.00983.x
Search in Google Scholar Back to article
YUSNITA, Y. – JAMALUDIN, J. – AGUSTIANSYAH, A. – HAPSORO, D. 2017. A combination of IBA and NAA resulted in better rooting and shoot sprouting than single auxin on malay apple [Syzygium malaccense (L.) Merr. & Perry] stem cuttings. In AGRIVITA, Journal of Agricultural Science, vol. 40, no. 1, pp. 80 – 90.10.17503/agrivita.v40i0.1210
Search in Google Scholar Back to article

Optimizing Micropropagation of Apple (Malus × Domestica Borkh) and in Vitro Root Induction By Piriformospora indica

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