Responses of Two Barley Subspecies to In vitro-Induced Heavy Metal Stress: Seeds Germination, Seedlings Growth and Cytotoxicity Assay

Nouri, Mohamed; El Rasafi, Taoufik; Haddioui, Abdelmajid

doi:10.2478/agri-2019-0011

Abstract

In this work three heavy metals: cadmium (as CdSO₄), cobalt (as CoCl₂) and zinc (as ZnSO₄), were used to determine and compare their toxicity towards two subspecies of barley (Hordeum vulgare subsp. vulgare L. and Hordeum vulgare subsp. distichum L.), focusing on seeds germination, seedlings growth, and cytological parameters. The results indicate that the effect of these heavy metals depends on the metal kind, the metal concentrations and the plant subspecies. Generally, in the case of H. vulgare, the heavy metal salts understudy did not influence significantly seed germination and seedling growth parameters. However, these metal salts influence significantly these parameters for H. distichum. The cytological test showed significant decrease (p < 0.05) in the mitotic index among the increase of the heavy metal concentrations when evaluated with the control for H. vulgare and H. distichum. Consequently, H. vulgare seemed to be more tolerant of the increase of the three heavy metals concentrations than H. distichum.

References

ADREES, M. – ALI, S. – RIZWAN, M. – IBRAHIM, M. – ABBAS, F. – FARID, M. – ZIA-UR-REHMAN, M. – IRSHAD, M.K. – BHARWANA, S.A. 2015. The effect of excess copper on growth and physiology of important food crops: a review. In Environmental Science and Pollution Research, vol. 22, pp. 8148 – 8162.10.1007/s11356-015-4496-5
Search in Google Scholar Back to article
AKINCI, I.E. – AKINCI, S. 2010. Effect of chromium toxicity on germination and early seedling growth in melon (Cucumis melo L.). In African Journal of Biotechnology, vol. 9, pp. 4589 – 4594.
Search in Google Scholar Back to article
AL-SABTI, K. – KURELEC, B. 1985. Chromosomal aberrations in onion (Allium cepa) induced by water chlorination by-products. In Bulletin of Environmental Contamination and Toxicology, vol. 34, pp. 80 – 88.10.1007/BF01609706
Search in Google Scholar Back to article
ARTICO, L.L. – KOMMLING, G. – MIGITA, N.A. – MENEZES, A.P.S. 2018. Toxicological effects of surface water exposed to coal contamination on the test system Allium cepa. In Water, Air, & Soil Pollution, vol. 229, pp. 248.10.1007/s11270-018-3904-0
Search in Google Scholar Back to article
AWIKA, J.M. 2011. Major cereal grains production and use around the world. In Advances in cereal science: implications to food processing and health promotion, Chapter 1, pp: 1 – 13, Washington, DC: ACS Symposium Series; American Chemical Society.10.1021/bk-2011-1089.ch001
Search in Google Scholar Back to article
BADERNA, D. – LOMAZZI, E. – POGLIAGHI, A. – CIACCIA, G. – LODI, M. – BENFENATI, E. 2015. Acute phytotoxicity of seven metals alone and in mixture: Are Italian soil threshold concentrations suitable for plant protection? In Environmental Research, vol. 140, pp. 102 – 111.10.1016/j.envres.2015.03.023
Search in Google Scholar Back to article
BAIK, B.-K. – ULLRICH, S.E. 2008. Barley for food: Characteristics, improvement, and renewed interest. In Journal of Cereal Science, vol. 48, pp. 233 – 242.10.1016/j.jcs.2008.02.002
Search in Google Scholar Back to article
BIRDSALL, M. – MACLEOD, R.D. 1990. Early growth of the root system in Allium cepa. In Canadian Journal of Botany, vol. 68, pp. 747 – 753.10.1139/b90-099
Search in Google Scholar Back to article
CALABRESE, E.J. – BLAIN, R.B. 2009. Hormesis and plant biology. In Environmental Pollution, vol. 157, pp. 42 – 48.10.1016/j.envpol.2008.07.028
Search in Google Scholar Back to article
CHEN, F. – WANG, F. – ZHANG, G. – WU, F. 2008. Identification of barley varieties tolerant to cadmium toxicity. In Biological Trace Element Research, vol. 121, pp. 171–179.10.1007/s12011-007-8042-2
Search in Google Scholar Back to article
COP (Cherifian Office of Phosphates) 2017. Annuel Report. Avaiable at: 2017 (https://www.ocpgroup.ma/sites/default/files/2018-11/RA%20OCP%202017%20VF.pdf
Search in Google Scholar Back to article
DANERI-CASTRO, S.N. – SVENSSON, B. – ROBERTS, T.H. 2016. Barley germination: spatio-temporal considerations for designing and interpreting ‘omics’ experiments. In Journal of Cereal Science, vol. 70, pp. 29 – 37.10.1016/j.jcs.2016.05.012
Search in Google Scholar Back to article
DATTA, S. – SINGH, J. – SINGH, J. – SINGH, S. – SINGH, S. 2018. Assessment of genotoxic effects of pesticide and vermicompost treated soil with Allium cepa test. In Sustainable Environment Research, vol. 28, pp. 171 – 178.10.1016/j.serj.2018.01.005
Search in Google Scholar Back to article
EBBS, S.D. – KOCHIAN, L.V. 1998. Phytoextraction of zinc by oat (Avena sativa), barley (Hordeum vulgare), and indian mustard (Brassica juncea). In Environmental Science & Technology, vol. 32, pp. 802 – 806.10.1021/es970698p
Search in Google Scholar Back to article
FAOSTAT, 2017. Food and Agriculture Organization of the United Nations, http://www.fao.org/faostat/en/#data/QC/visualize
Search in Google Scholar Back to article
FERNANDES, T.C.C. – MAZZEO, D.E.C. – MARIN-MORALES, M.A. 2007. Mechanism of micronuclei formation in polyploidizated cells of Allium cepa exposed to trifluralin herbicide. In Pesticide Biochemistry and Physiology, vol. 88, pp. 252 – 259.10.1016/j.pestbp.2006.12.003
Search in Google Scholar Back to article
FISKESJÖ, G. 1985. Allium test on river water from Braån and Saxån before and after closure of a chemical factory. In Ambio, vol. 14, pp. 99 – 103.
Search in Google Scholar Back to article
GAPPER, C. – DOLAN, L. 2006. Control of plant development by reactive oxygen species. In Plant Physiology, vol. 141, pp. 341 – 345.10.1104/pp.106.079079
Search in Google Scholar Back to article
GERAS’KIN, S. – CHURYUKIN, R. – VOLKOVA, P. 2017. Radiation exposure of barley seeds can modify the early stages of plants’ development. In Journal of Environmental Radioactivity, vol. 177, pp. 71 – 83.10.1016/j.jenvrad.2017.06.008
Search in Google Scholar Back to article
HAGNER, M. – ROMANTSCHUK, M. – PENTTINEN, O.P. – EGFORS, A. – MARCHAND, C. – AUGUSTSSON, A. 2018. Assessing toxicity of metal contaminated soil from glassworks sites with a battery of biotests. In Science of the Total Environment, vol. 613 – 614, pp. 30 – 38.10.1016/j.scitotenv.2017.08.121
Search in Google Scholar Back to article
HAKKOU, R. – BENZAAZOUA, M. – BUSSIÈRE, B. 2016. Valorization of phosphate waste rocks and sludge from the Moroccan phosphate mines: challenges and perspectives. In Procedia Engineering, vol. 138, pp. 110 – 118.10.1016/j.proeng.2016.02.068
Search in Google Scholar Back to article
HE, S. – YANG, X. – HE, Z. – BALIGAR, V.C. 2017. Morphological and physiological responses of plants to cadmium toxicity: a review. In Pedosphere, vol. 27, pp. 421 – 438.10.1016/S1002-0160(17)60339-4
Search in Google Scholar Back to article
ICARDA (International Center for Agricultural Research in & the Dry Areas). 2005. Morocco and ICARDA: Collaboration since 1977, second (Ed ICARDA). Aleppo, Syria.
Search in Google Scholar Back to article
ICARDA (International Center for Agricultural Research in the Dry Areas. 2017. ANNUAL REPORT 2016 Enhancing Resilience: Helping Dryland Communities to Thrive. Beirut, Lebanon: ICARDA.
Search in Google Scholar Back to article
IKIĆ, I. – MARIČEVIĆ, M. – TOMASOVIĆ, S. – GUNJAČA, J. – ŠATOVIĆ, Z. – ŠARČEVIĆ, H. 2012. The effect of germination temperature on seed dormancy in Croatian-grown winter wheats. In Euphytica, vol. 188, pp. 25 – 34.10.1007/s10681-012-0735-8
Search in Google Scholar Back to article
KOPYRA, M. – GWÓŹDŹ, E.A. 2003. Nitric oxide stimulates seed germination and counteracts the inhibitory effect of heavy metals and salinity on root growth of Lupinus luteus. In Plant Physiology and Biochemistry, vol. 41, pp. 1011 – 1017.10.1016/j.plaphy.2003.09.003
Search in Google Scholar Back to article
LEFÈVRE, I. – MARCHAL, G. – CORRÉAL, E. – ZANUZZI, A. – LUTTS, S. 2009. Variation in response to heavy metals during vegetative growth in Dorycnium pentaphyllum Scop. In Plant Growth Regulation, vol. 59, pp. 1 – 11.10.1007/s10725-009-9382-z
Search in Google Scholar Back to article
LIANG, C. – XIAO, H. – HU, Z. – ZHANG, X. – HU, J. 2018. Uptake, transportation, and accumulation of C60 fullerene and heavy metal ions (Cd, Cu, and Pb) in rice plants grown in an agricultural soil. In Environmental Pollution, vol. 235, pp. 330 – 338.10.1016/j.envpol.2017.12.062
Search in Google Scholar Back to article
MADZHUGINA, Y.G. – KUZNETSOV, V.V. – SHEVYAKOVA, N.I. 2008. Plants inhabiting polygons for megapolis waste as promising species for phytoremediation. In Russian Journal of Plant Physiology, vol. 55, pp. 410 – 419.10.1134/S1021443708030187
Search in Google Scholar Back to article
MARTÍNEZ-MORENO, F. – SOLIS, I. – IGARTUA, E. 2017. Barley Types and varieties in Spain: a historical overview. In Ciencia e Investigación Agraria, vol. 44, pp. 1 – 12.10.7764/rcia.v44i1.1638
Search in Google Scholar Back to article
MESI, A. – KOPLIKU, D. 2013. Cytotoxic and genotoxic potency screening of two pesticides on Allium cepa L. In Procedia Technology, vol. 8, pp. 19 – 26.10.1016/j.protcy.2013.11.005
Search in Google Scholar Back to article
MIRALLES, P. – CHURCH, T.L. – HARRIS, A.T. 2012. Toxicity, uptake, and translocation of engineered nanomaterials in vascular plants. In Environmental Science & Technology, vol. 46, pp. 9224 – 9239.10.1021/es202995d
Search in Google Scholar Back to article
NAGAJYOTI, P.C. – LEE, K.D. – SREEKANTH, T.V.M. 2010. Heavy metals, occurrence and toxicity for plants: A review. In Environmental Chemistry Letters, vol. 8, pp. 199 – 216.10.1007/s10311-010-0297-8
Search in Google Scholar Back to article
NOURI, M. – HADDIOUI, A. 2016. Human and animal health risk assessment of metal contamination in soil and plants from Ait Ammar abandoned iron mine, Morocco. In Environmental Monitoring and Assessment, vol. 188, pp. 6.10.1007/s10661-015-5012-6
Search in Google Scholar Back to article
OZDENER, Y. – KUTBAY, H.G. 2009. Toxicity of copper, cadmium, nickel, lead and zinc on seed germination and seedling growth in Eruca sativa. In Fresenius Environmental Bulletin, vol. 18, pp. 26 – 31.
Search in Google Scholar Back to article
DA ROS, C. – LIBRALATO, G. – GHIRARDINI, A.V. – RADAELLI, M. – CAVINATO, C. 2018. Assessing the potential phytotoxicity of digestate from winery wastes. In Ecotoxicology and Environmental Safety, vol. 150, pp. 26 – 33.10.1016/j.ecoenv.2017.12.029
Search in Google Scholar Back to article
SENEVIRATNE, M. – RAJAKARUNA, N. – RIZWAN, M. – MADAWALA, H.M.S.P. – OK, Y.S. – VITHANAGE, M. 2019. Heavy metal-induced oxidative stress on seed germination and seedling development: a critical review. In Environmental Geochemistry and Health, vol. 41, pp. 1813–1831. https://doi.org/10.1007/s10653-017-0005-810.1007/s10653-017-0005-828702790
Open DOI Search in Google Scholar Back to article
SEREGIN, I.V. – KOZHEVNIKOVA, A.D. 2005. Distribution of cadmium, lead, nickel, and strontium in imbibing maize caryopses. In Russian Journal of Plant Physiology, vol. 52, pp. 565 – 569.10.1007/s11183-005-0084-8
Search in Google Scholar Back to article
SHAHZAD, B. – TANVEER, M. – CHE, Z. – REHMAN, A. – CHEEMA, S.A. – SHARMA, A. – SONG, H. – REHMAN, S. – ZHAORONG, D. 2018. Role of 24-epibrassinolide (EBL) in mediating heavy metal and pesticide induced oxidative stress in plants: A review. In Ecotoxicology and Environmental Safety, vol. 147, pp. 935 – 944.10.1016/j.ecoenv.2017.09.066
Search in Google Scholar Back to article
SINGH, R. – UPADHYAY, A.K. – SINGH, D.P. 2018. Regulation of oxidative stress and mineral nutrient status by selenium in arsenic treated crop plant Oryza sativa. In Ecotoxicology and Environmental Safety, vol. 148, pp. 105–113.10.1016/j.ecoenv.2017.10.008
Search in Google Scholar Back to article
STREET, R.A. – KULKARNI, M.G. – STIRK, W.A. – SOUTHWAY, C. – VAN STADEN, J. 2007. Toxicity of metal elements on germination and seedling growth of widely used medicinal plants belonging to Hyacinthaceae. In Bulletin of Environmental Contamination and Toxicology, vol. 79, pp. 371 – 376.10.1007/s00128-007-9237-0
Search in Google Scholar Back to article
TAMÁS, L. – MISTRÍK, I. – ZELINOVÁ, V. 2017. Heavy metal-induced reactive oxygen species and cell death in barley root tip. In Environmental and Experimental Botany, vol. 140, pp. 34–40.10.1016/j.envexpbot.2017.05.016
Search in Google Scholar Back to article
TÜRKOĞLU, Ş. 2008. Evaluation of genotoxic effects of sodium propionate, calcium propionate and potassium propionate on the root meristem cells of Allium cepa. In Food and Chemical Toxicology, vol. 46, pp. 2035 – 2041.10.1016/j.fct.2008.01.043
Search in Google Scholar Back to article
WANG, W. 1991. Literature review on higher plants for toxicity testing. In Water, Air, and Soil Pollution, vol. 59, pp. 381 – 400.10.1007/BF00211845
Search in Google Scholar Back to article
WANG, C.R. – TIAN, Y. – WANG, X.R. – YU, H.X. – LU, X.W. – WANG, C. – WANG, H. 2010. Hormesis effects and implicative application in assessment of lead-contaminated soils in roots of Vicia faba seedlings. In Chemosphere, vol. 80, pp. 965 – 971.10.1016/j.chemosphere.2010.05.049
Search in Google Scholar Back to article
ZUCCONI, F. – PERA, A. – FORTE, M. – BERTOLDI, M. 1981. Evaluating toxicity of immature compost. In Biocycle, vol. 22, pp. 54 – 57.
Search in Google Scholar Back to article

Responses of Two Barley Subspecies to In vitro-Induced Heavy Metal Stress: Seeds Germination, Seedlings Growth and Cytotoxicity Assay

Abstract

Paradigm

My account