Characterization and Evaluation of Bacillus siamensis Isolate for its Growth Promoting Potential in Tomato

Islam, Amanul; Kabir, Shahinur; Khair, Abul

doi:10.2478/agri-2019-0005

Abstract

In quest of prospective rizospheric bacteria of agricultural importance, one of the isolates from bean (Lablab niger Medikus) was identified as Bacillus siamensis based on morphological, biochemical and 16S rRNA gene sequencing data. Study was carried out to evaluate growth promotion of two tomato cultivars, in vitro and in vivo. Experiments conducted for two consecutive years, following seed treatments revealed that the bacterial isolate increased plant height by 14.66‒15.68%, shoot fresh weight by 34.5‒65.09% and root fresh weight by 75.3‒92.48% over the non-treated control. The bacterial strain showed encouraging results for plant growth promotion in pot study and hence may be useful for the growth enhancement of tomato plant.

References

ALAM, M.S. – CUI, Z. – YAMAGISHI, T. – ISHI, R. 2003. Rice cultivar variation in the growth response to inoculation of free-living rhizobacteria. In Plant Production Science, vol. 6, pp. 50 ‒ 51.10.1626/pps.6.50
Search in Google Scholar Back to article
ALAN, C.G. – KIRAN, R.G. 2018. Plant growth-promoting rhizobacteria promote plant size inequality. In Scientific REPORTs, vol. 8, pp. 13828. DOI: 10.1038/s41598-018-32111-z.10.1038/s41598-018-32111-z
Search in Google Scholar Back to article
ALMONEAFY, A. – XIE, G.L. – TIAN, W.X. – XU, L.H. – ZHANG, G.Q. – IBRAHIM, M. 2012. Characterization and evaluation of Bacillus isolates for their potential plant growth and biocontrol activities against tomato bacterial wilt. In African Journal of Biotechnology, vol. 11, no. 28, pp. 7193 ‒ 7201.
Search in Google Scholar Back to article
ALMONEAFY, A.A. – KAKAR, K.U. – LI, B. – SAAND, M.A. – CHUN-lAN, Y. – XIE, G.L. 2014. Tomato plant growth promotion and antibacterial related-mechanisms of four rhizobacterial Bacillus strains against Ralstonia solanacearum. In Symbiosis, vol. 63, no. 2, pp. 59 ‒ 70. DOI: 10.1007/s13199-014-0288-9.10.1007/s13199-014-0288-9
Open DOI Search in Google Scholar Back to article
AMBAWADE, M.S. – PATHADE, G.R. 2018. Indole Acetic Acid (IAA) Production by Bacillus siamensis BE 76 isolated from Musa balbisiana (Banana). In Journal of Chemical and Pharmaceutical Research, vol. 10, no. 2, pp. 610.
Search in Google Scholar Back to article
AMBAWADE, M.S. – PATHADE, G.R. 2015. Production of gibberellic acid by Bacillus siamensis BE 76 isolated from banana plant (Musa spp.). In International Journal of Science and Research, vol. 4, no. 7, pp. 394 – 398.
Search in Google Scholar Back to article
ARNON, D.I. 1949. Copper enzymes in isolated chloroplasts polyphenol oxidase in Beta vulgaris. In Plant Physiology, vol. 24, no. 1, pp. 1 – 15.10.1104/pp.24.1.1
Search in Google Scholar Back to article
BAHIG, A.E. – ALY, E.A. – KHALED, A.A. – AMEL, K.A. 2008. Isolation, characterization and application of bacterial population from agricultural soil at Sohag Province, Egypt. In Malaysian Journal of Microbiology, vol. 4, no. 2, pp. 42 – 50.10.21161/mjm.11808
Search in Google Scholar Back to article
BAKKER, A.W. – SCHIPPERS, B. 1987. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. In Soil Biology and Biochemistry, vol. 19, pp. 451–457. DOI: 10.1016/0038-0717(87)90037-X10.1016/0038-0717(87)90037-X
Open DOI Search in Google Scholar Back to article
BBS (Bangladesh Bureau of Statistics). 2016. Yearbook of Agricultural Statistics-2015. Bangladesh Bureau of Statistics, Ministry of Planning, Government of the People’s Republic of Bangladesh.
Search in Google Scholar Back to article
CABRA, C.T. – GONZALEZ, C.A.R. – CUASQUER, C.P.V. – ALZATE, O.A.T. – RODRIGUEZ, A.H. 2017. Bacillus effect on the germination and growth of tomato seedlings (Solanum lycopersicum L). In Acta Biolόgica Colombiana, vol. 22, no. 1, pp. 37 – 44.10.15446/abc.v22n1.57375
Search in Google Scholar Back to article
CHOOKIETWATTANA, K. – MANEEWAN, K. 2012. Selection of efficient salt-tolerant bacteria containing ACC deaminase for promotion of tomato growth under salinity stress. In Soil and Environment, vol. 31, no. 1, pp. 30 ‒ 36.
Search in Google Scholar Back to article
FIGUEIREDO, Md. V.B. – BONIFACIO, A. – RODRIGUES, A.C. – ARAUJO, F.Fde. 2016. Plant growth-promoting rhizobacteria: Key mechanisms of action. In CHOUDHARY, D.K. (Ed.) ‒ VARMA, A. Microbial-mediated induced systemic resistance in plants. Singapore: Springer, pp. 27–33. DOI: 10.1007/978-981-10-0388-2_3.10.1007/978-981-10-0388-2_3
Open DOI Search in Google Scholar Back to article
FIGUEROA-LOPEZ, A.M. – CORDERO-RAMIREZ, J.D. – MARTINEZ-ÁLVAREZ, J.C. – LOPEZ-MEYER, M. – LIZARRAGA-SANCHEZ, G.J. – FELIX-GASTELUM, R. – CASTRO-MARTINEZ, C. – MALDONADO-MENDOZA, I.E. 2016. Rhizospheric bacteria of maize with potential for biocontrol of Fusarium verticillioides. In Springer Plus, vol. 5, pp. 330. DOI: 10.1186/s40064-016-1780-x.10.1186/s40064-016-1780-x479282027066355
Open DOI Search in Google Scholar Back to article
ISTA. 1999. International rules for seed testing. In Seed Science and Technology, vol. 27, Supplement rules, pp. 27 – 31.
Search in Google Scholar Back to article
KHALEQUZZAMAN, K.M. – JINNAH, M.A. – RASHID, M.A.A.M. – CHOWDHURY, M.N.A. – ALAM, M.M. 2002. Effect of Pseudomonas fluorescens in controlling bacterial wilt of tomato. In Plant Pathology Journal, vol. 1, pp. 71 – 73.10.3923/ppj.2002.71.73
Search in Google Scholar Back to article
KHALID, A. – ARSHAD, M. – ZAHIR, Z.A. 2004. Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat. In Journal of Applied Microbiology, vol. 96, pp. 473 – 480. DOI:10.1046/j.1365-2672.2003.02161.x10.1046/j.1365-2672.2003.02161.x14962127
Open DOI Search in Google Scholar Back to article
KLOEPPER, J.W. – RYU, C.M. – ZHANG, S. 2004. Induced systemic resistance and promotion plant growth by Bacillus spp. In Phytopathology, vol. 94, pp. 1259 – 1266.10.1094/PHYTO.2004.94.11.1259
Search in Google Scholar Back to article
KUMAR, P. – DUBEY, R.C. – MAHESHWARI, D.K. 2012. Bacillus strains isolated from rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens. In Microbiological Research, vol. 167, no. 8, pp. 493 – 499. DOI: 10.1016/j.micres.2012.05.00210.1016/j.micres.2012.05.00222677517
Open DOI Search in Google Scholar Back to article
KUMAR, S. – STECHER, G. – TAMURA, K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. In Molecular Biology and Evolution, vol. 33, no. 7, pp. 1870 – 1874.10.1093/molbev/msw054
Search in Google Scholar Back to article
MAURHOFER, M. – KEEL, C. – HAAS, D. – DEFAGO, G. 1995. Influence of plant species on disease suppression by Pseudomonas fluorescens strain CHA0 with enhanced production. In Plant Pathology, vol. 44, no. 1, pp. 40 – 50. DOI: 10.1111/j.1365-3059.1995.tb02714.x10.1111/j.1365-3059.1995.tb02714.x
Search in Google Scholar Back to article
NAIK, P. – RAMAN, G. – NARAYANAN, K. – SAKTHIVEL, N. 2008. Assessment of genetic and functional diversity of phosphate solubilizing fluorescent pseudomonads isolated from rhizospheric soil. In BMC Microbiology, vol. 8, pp. 230.10.1186/1471-2180-8-230
Search in Google Scholar Back to article
PAINTER, P.R. – MARR, A.G. 1968. Mathematics of microbial populations. In Annual Review of Microbiology, vol. 22, pp. 519 – 548.10.1146/annurev.mi.22.100168.002511
Search in Google Scholar Back to article
SANA, H. – ANJUM, T. – FATIMA, S. – ALI, A. – MAHBOOB, A. – AKRAM, W. 2014. Potential of some native Bacillus strains to promote growth of tomato. In Pakistan Journal of Biotechnology, vol. 11, no. 2, pp. 153 – 162.
Search in Google Scholar Back to article
SCHAAD, N.W. – JONES, J.B. – CHUN, W. 2001. Laboratory guide for the identification of plant pathogenic bacteria. St. Paul, MN : APS Press, pp.1 – 58.
Search in Google Scholar Back to article
SHIVAKUMAR, S. – BHAKTHAVATCHALU, S. – SULLIA, S.B. 2013. Characterization of multiple plant growth promotion traits of Pseudomonas aeruginosa FP6, a potential stress tolerant biocontrol agent. In Annals of Biological Research, vol. 4, no. 2, pp. 214 – 223.
Search in Google Scholar Back to article
SIVASAKTHI, S. – USHARANI, G. – SARANRAJ, P. 2014. Biocontrol potentiality of plant growth promoting bacteria (PGPR)-Pseudomonas fluorescens and Bacillus subtilis: a review. In African Journal of Agricultural Research, vol. 9, no. 16, pp. 1265 – 1277.
Search in Google Scholar Back to article
SICUIA, O.A. – GROSU, I. – CONSTANTINESCU, F. – VOAIDES, C. – CORNEA, C.P. 2015. Enzymatic and genetic variability in Bacillus spp. strains with plant beneficial qualities. In Agrolife Scientific Journal, vol. 4, no. 2, pp. 124 – 131.
Search in Google Scholar Back to article
SILVA, H.S.A. – ROMEIRO, R.S. – MOUNTEER, A. 2003. Development of a root colonization bioassay for rapid screening of rhizobacteria for potential biocontrol agents. In Journal of Phytopathology, vol. 151, pp. 42 – 46.10.1046/j.1439-0434.2003.00678.x
Search in Google Scholar Back to article
STANIER, R.Y. – DOUDOROFF, M. – ADELBERG, E.A. 1970. General microbiology. London, UK : Macmillan & Co., Ltd, pp. 302 – 306.10.1007/978-1-349-15408-1
Search in Google Scholar Back to article
STEIN, T. 2005. Bacillus subtilis antibiotics: structures, synthesis and specifics functions. In Molecular Microbiology, vol. 56, pp. 845 – 857.10.1111/j.1365-2958.2005.04587.x
Search in Google Scholar Back to article
TAMURA, K. – NEI, M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. In Molecular Biology and Evolution, vol. 10, no. 3, pp. 512 – 526.
Search in Google Scholar Back to article
VESSEY, J.K. – BUSS, T.J. 2002. Bacillus cereus UW85 inoculation effects on growth, nodulation, and N accumulation in grain legumes: controlled-environment studies. In Canadian Journal of Plant Science, vol. 82, no. 2, pp. 282 – 290.10.4141/P01-047
Search in Google Scholar Back to article
WELLER, D.M. – COOK, R.J. 1983. Suppression of take-all of wheat by seed treatments with fluorescent Pseudomonads. In Phytopathology, vol. 73, pp. 463 – 469.10.1094/Phyto-73-463
Search in Google Scholar Back to article
YOON, S.H. – HA, S.M. – KWON, S. – LIM, J. – KIM, Y. – SEO, H. – CHUN, J. 2017. Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. In International Journal of Systematic and Evolutionary Microbiology, vol. 67, pp. 1613 – 161.10.1099/ijsem.0.001755
Search in Google Scholar Back to article

Characterization and Evaluation of Bacillus siamensis Isolate for its Growth Promoting Potential in Tomato

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