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DOPC + OTAB + DNA Complexes – Effect of Ionic Strength and Surface Charge Density on DNA Condensation Cover

DOPC + OTAB + DNA Complexes – Effect of Ionic Strength and Surface Charge Density on DNA Condensation

European Pharmaceutical Journal
Volume 72 (2025): Issue 1 (September 2025)
By: Marcela Chovancová,  Lukáš Hubčík,  Karin Babošová,  Mária Klacsová and  Daniela Uhríková  
Open Access
|May 2025

Figures & Tables

Figure 1.

Structures of DOPC, OTAB and EtBr.DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine, OTAB: octadecyltrimethylammonium bromide, EtBr: 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide
Structures of DOPC, OTAB and EtBr.DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine, OTAB: octadecyltrimethylammonium bromide, EtBr: 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide

Figure 2.

(a) Dependences of the emission fluorescence intensity I of complex DNA + EtBr on DNA/EtBr mole ratio at different NaCl ionic strengths. Error bars are within the size of the symbols. Lines represent the best linear fits of respective experimental data. (b) Dependences of the fluorescence intensity I of DNA/EtBr = 5:1 mol mol−1 complex (▪) on the ionic strength compared to pure EtBr (). Curves are a guide to the eyes only.
(a) Dependences of the emission fluorescence intensity I of complex DNA + EtBr on DNA/EtBr mole ratio at different NaCl ionic strengths. Error bars are within the size of the symbols. Lines represent the best linear fits of respective experimental data. (b) Dependences of the fluorescence intensity I of DNA/EtBr = 5:1 mol mol−1 complex (▪) on the ionic strength compared to pure EtBr (). Curves are a guide to the eyes only.

Figure 3.

(a) Dependence of normalized fluorescence intensity Inorm of DOPC + OTAB + DNA + EtBr complexes on the OTAB/DNA charge ratio at different OTAB/DOPC mole ratios in 5 mmol l−1 NaCl. The points are connected to guide the eyes. Error bars are smaller than the symbol size. (b) Dependence of normalized fluorescence intensity Inorm of DOPC + OTAB + DNA + EtBr complexes on ionic strength at a charge ratio of OTAB/DNA = 10 and at different OTAB/DOPC mole ratios.
(a) Dependence of normalized fluorescence intensity Inorm of DOPC + OTAB + DNA + EtBr complexes on the OTAB/DNA charge ratio at different OTAB/DOPC mole ratios in 5 mmol l−1 NaCl. The points are connected to guide the eyes. Error bars are smaller than the symbol size. (b) Dependence of normalized fluorescence intensity Inorm of DOPC + OTAB + DNA + EtBr complexes on ionic strength at a charge ratio of OTAB/DNA = 10 and at different OTAB/DOPC mole ratios.

Figure 4.

The SAXS patterns of DOPC in 5 and 150 mM of NaCl at 20 °C (a) and 50 °C (b). Intensity is in a logarithmic scale.
The SAXS patterns of DOPC in 5 and 150 mM of NaCl at 20 °C (a) and 50 °C (b). Intensity is in a logarithmic scale.

Figure 5.

The SAXS patterns of DOPC + OTAB + DNA complexes at different ionic strengths and mole ratios OTAB/DOPC = 1 (a) and 0.3 (b) at 20 °C. For illustration, the data points are simulated with the best Lorentzian fit curves. Intensity is in a logarithmic scale.
The SAXS patterns of DOPC + OTAB + DNA complexes at different ionic strengths and mole ratios OTAB/DOPC = 1 (a) and 0.3 (b) at 20 °C. For illustration, the data points are simulated with the best Lorentzian fit curves. Intensity is in a logarithmic scale.

Figure 6.

Dependence of the repeat distance d of the lipid bilayers stacking in the DOPC + OTAB + DNA system on ionic strength at the mole ratios OTAB/DOPC = 0.3 (dots) and 1 (circles) at 20 °C (blue line) and 50 °C (red line).
Dependence of the repeat distance d of the lipid bilayers stacking in the DOPC + OTAB + DNA system on ionic strength at the mole ratios OTAB/DOPC = 0.3 (dots) and 1 (circles) at 20 °C (blue line) and 50 °C (red line).
DOI: https://doi.org/10.2478/afpuc-2025-0003 | Journal eISSN: 2453-6725
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Language: English
Page range: 31 - 40
Submitted on: Jan 13, 2025
Accepted on: Apr 4, 2025
Published on: May 18, 2025
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
DNA,
DOPC,
octadecyltrimethylammonium bromide,
ionic strength,
fluorescence spectroscopy,
SAXS/WAXS
Related subjects:
Pharmacy,
Pharmacy, other

© 2025 Marcela Chovancová, Lukáš Hubčík, Karin Babošová, Mária Klacsová, Daniela Uhríková, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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