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Characterisation of the lipolytic enzymatic activities of fungal rizoenzymes from Rhizopus oryzae in comparison to pancreatin from pigs Cover

Characterisation of the lipolytic enzymatic activities of fungal rizoenzymes from Rhizopus oryzae in comparison to pancreatin from pigs

Open Access
|Aug 2024

Figures & Tables

Figure 1.

Fatty acids profile of the olive oil used after total hydrolysis.

Table 1.

Composition of the olive oil used after total hydrolysis.

Fatty acidOlive oila (%)Analysed valuesb (%)
14:0≤0.05–0.5
16:07.5–20.013.3
16:10.3–3.51.3
17:0≤0.05–0.3
17:1≤0.05–0.3
18:00.5–5.0Not considered
18:155.0–83.073.2
18:23.5–21.010.8
18:3≤0.05–1.01.0
20:0≤0.05–0.6
20:1≤0.05–0.40.4
20:2≤0.05
22:0≤0.05–0.2
22:1≤0.05
22:2≤0.05
24:0≤0.05–0.2
24:1≤0.05

a The values correspond to the trade standard applying to olive oil and olive pomace oil of the International Olive Oil Council (Madrid) COI/T.15/NC no. 3/Rev. 2; 24 November 2006.

b Calculated from the total release at pH 7 with pancreatin and rizoenzymes.

Figure 2.

LC–MS/MS chromatogram of olive oil after Pangrol enzyme reaction at pH = 7 overlayed by a total hydrolysis chromatogram of the same sample.

Figure 3.

Total fatty acids conversion rate (sum of overall fatty acids) as a function of pH for both enzyme preparations.

Figure 4.

Conversion rate of single fatty acids as a function of pH for both enzyme preparations (rizoenzymes (A) and pancreatin (B)).

Figure 5.

Total fatty acids conversion rate (sum of overall fatty acids) as a function of taurocholate concentration (mmol/L) for both enzyme preparations.

Figure 6.

Conversion rate of the single fatty acids as a function of the taurocholate concentration (mM) for both enzyme preparations (rizoenzymes (A) and pancreatin (B)).

DOI: https://doi.org/10.2478/afpuc-2024-0009 | Journal eISSN: 2453-6725 (formerly 1338-6786) | Journal ISSN: 0301-2298
Language: English
Page range: 53 - 60
Submitted on: Feb 28, 2024
Accepted on: Jun 11, 2024
Published on: Aug 10, 2024
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
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© 2024 C. Schön, R. Wacker, M. Rothe, B. Lipowicz, A. Iphöfer, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.