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Experimental evaluation of the nonselective and selective TMEM16 family calcium-activated chloride channel blockers in the airways Cover

Experimental evaluation of the nonselective and selective TMEM16 family calcium-activated chloride channel blockers in the airways

European Pharmaceutical Journal
Volume 70 (2023): Issue 1 (December 2023)
By: J. Mažerik,  L. Smieško,  L. Fedorová and  E. Gondáš  
Open Access
|Sep 2023

Figures & Tables

Figure 1.

Comparison of the changes in the number of AC-induced coughs by OVA sensitization and different treatments. Tested drugs were administered by inhalation (A, B) or intraperitoneally (C, D). The measurements of response on the inhalation of substances were performed after 60 and 120 min, and the effect when they were administered intraperitoneally was evaluated after 60 and 240 min. For the statistical analysis, one-way ANOVA and Bonferroni post hoc test were used.*p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 versus negative control group (saline); +p ≤ 0.05, ++p ≤ 0.01, and +++p ≤ 0.001 negative control group versus positive control group (codeine).
Comparison of the changes in the number of AC-induced coughs by OVA sensitization and different treatments. Tested drugs were administered by inhalation (A, B) or intraperitoneally (C, D). The measurements of response on the inhalation of substances were performed after 60 and 120 min, and the effect when they were administered intraperitoneally was evaluated after 60 and 240 min. For the statistical analysis, one-way ANOVA and Bonferroni post hoc test were used.*p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 versus negative control group (saline); +p ≤ 0.05, ++p ≤ 0.01, and +++p ≤ 0.001 negative control group versus positive control group (codeine).

Figure 2.

Comparison of sRaw values in healthy OVA-sensitized guinea pigs treated with TMEM16 blockers and control drugs when challenged with inhalational histamine and methacholine. The substances were administered by inhalation or intraperitoneally.#p ≤ 0.05 versus salbutamol; for further explanation of statistical analysis, see legend to Fig. 1.
Comparison of sRaw values in healthy OVA-sensitized guinea pigs treated with TMEM16 blockers and control drugs when challenged with inhalational histamine and methacholine. The substances were administered by inhalation or intraperitoneally.#p ≤ 0.05 versus salbutamol; for further explanation of statistical analysis, see legend to Fig. 1.

Figure 3.

Results of CBF analysis. The changes in values were monitored on samples obtained from healthy and OVA-sensitized, untreated animals and 100 μl of substances was administered directly to the samples in the same concentration that was used for inhalation. B-benzbromarone, A01-CaCCinh-A01, OVAS-negative control saline tested in sensitized airways, OVAB-benzbromarone tested in sensitized airways, and OVAA01-CaCCinh-A01 tested in sensitized airways. */+ p ≤ 0.05, one-way ANOVA and Bonferroni posthoc test.
Results of CBF analysis. The changes in values were monitored on samples obtained from healthy and OVA-sensitized, untreated animals and 100 μl of substances was administered directly to the samples in the same concentration that was used for inhalation. B-benzbromarone, A01-CaCCinh-A01, OVAS-negative control saline tested in sensitized airways, OVAB-benzbromarone tested in sensitized airways, and OVAA01-CaCCinh-A01 tested in sensitized airways. */+ p ≤ 0.05, one-way ANOVA and Bonferroni posthoc test.
DOI: https://doi.org/10.2478/afpuc-2023-0016 | Journal eISSN: 2453-6725
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Language: English
Page range: 1 - 7
Submitted on: May 19, 2023
Accepted on: Aug 14, 2023
Published on: Sep 30, 2023
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
calcium-activated chloride channels,
TMEM16A channel,
cough reflex,
bronchoconstriction,
mucociliary clearance,
guinea pigs
Related subjects:
Pharmacy,
Pharmacy, other

© 2023 J. Mažerik, L. Smieško, L. Fedorová, E. Gondáš, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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