Figure 1
Figure 2
Figure 3
Figure 4
| 0.1% formic acid aqueous solution in acetonitrile, % | Time, min |
|---|---|
| 95 | 1 |
| 70 | 4 |
| 50 | 7 |
| 95 | 2 |
Bioactive substances of Daucus carota subsp_ sativus fruits of Yaskrava, Nantska Kharkivska and Olenka species and of Dauci carotae subsp_ sativi fructuum extractum siccum_
| Compound | m/z of [M H]− | Daughter ion m/z | Content of phenolic compounds as absolutely dry matter, μg/g | |||
|---|---|---|---|---|---|---|
| Daucus carota subsp. sativus fruits | Dauci carotae subsp. sativi fructuum extractum siccum | |||||
| Yaskrava species | Olenka species | Nantska Kharkivska species | ||||
| Quinic acid | 191 | 85 | 1166.43 ± 29.16 | 502.83 ±12.57 | 680.20 ± 17.05 | 3997.56 ± 99.94 |
| 3-(3,4-Dihydroxycinnamoyl) quinic acid (chlorogenic acid) | 353 | 191 | 129.75 ± 3.24 | 39.87 ±1.00 | 242.48 ± 6.62 | 603.49 ± 15.09 |
| 3,4-Dihydroxycinnamic acid (R)-1-carboxy-2-(3,4-dihydroxyphenyl)ethyl ester (rosmarinic acid) | 359 | 161 | 0.71 ± 0.02 | 0.57 ± 0.01 | — | — |
| 3,4-Dihydroxybenzeneacrylic acid (caffeic acid) | 392 | 174 | — | — | — | 158.91 ± 3.97 |
| Luteolin-7-O-glucoside (cynaroside) | 447 | 285 | 99.57 ± 2.49 | 87.48 ± 2.19 | 80.45 ± 2.01 | 1997.36 ± 49.93 |
| Quercetin-3-O-rutinoside (rutin) | 609 | 300 | 14.91 ± 0.37 | 11.61 ± 0.27 | 11.50 ± 0.28 | — |
| Isorhamnetin-3-O-rutinoside (scolymoside) | 563 | 291 | 6.22 ± 0.16 | 5.48 ± 0.14 | 5.10 ± 0.12 | 124.77 ± 3.12 |
| 4′,5,7-Trihydroxyflavone (apigenin) | 269 | 117 | 0.25 ± 0.01 | — | — | 30.29 ± 0.76 |
| 3′-Methylquercetin (isorhamnetin) | 315 | 300 | 0.06 ± 0.01 | — | — | — |
| Apigenin 8-C-glucoside (vitexin) | 431.1 | 311 | 0.15 ± 0.01 | 0.11 ± 0.01 | 0.10 ± 0.01 | 4.17 ± 0.10 |
| Phloretin 2′-glucoside (phloridzin) | 435 | 273 | 0.09 ± 0.01 | — | 0.04 ± 0.01 | — |
| Kaempherol-3-O-glucoside (astragalin) | 447 | 284 | 3.15 ± 0.08 | 2.65 ± 0.07 | 2.68 ± 0.07 | 67.47 ± 1.69 |
| Quercetin 3-D-galactoside (hyperoside) | 451 | 344 | 15.73 ± 0.39 | 11.79 ± 0.29 | 12.23 ± 0.35 | 273.92 ± 6.85 |
| Isorhamnetin-3-O-glucoside | 477 | 314 | 6.86 ± 0.17 | 6.40 ± 0.16 | 5.75 ± 0.14 | 155.89 ± 3.90 |
| 3,5,7-Trihydroxyflavone (galangin) | 486 | 195 | 3.96 ± 0.10 | 2.99 ± 0.07 | 2.80 ± 0.07 | — |
| 3′,4′,5,6,7-Pentamethoxyflavone (sinensetin) | 414 | 272 | 0.10 ± 0.01 | 0.08 ± 0.01 | 0.06 ± 0.01 | — |
| Kaempferol 3-O-(6″-O-p-coumaroyl)glucopyranoside (tiliroside) | 528 | 233 | — | — | — | 33.05 ± 0.83 |
| Content of alicyclic acids | 1166.43 ± 29.16 | 502.83 ± 12.57 | 680.20 ± 17.05 | 3997.56 ± 99.94 | ||
| Total content of hydroxycinnamic acids | 130.46 ± 3.26 | 40.44 ± 1.01 | 242.48 ± 6.06 | 762.40 ± 19.06 | ||
| Total content of flavonoids | 151.05 ± 3.78 | 128.58 ± 3.21 | 120.71 ± 3.02 | 2686.92 ± 67.17 | ||
| Total content of phenolic compounds | 281.51 ± 7.04 | 169.02 ± 4.23 | 363.19 ± 9.09 | 3449.32 ± 86.23 | ||
| Total content of identified bioactive substances | 1447.94 ± 36.20 | 671.87 ± 16.80 | 1043.39 ± 26.08 | 7446.88 ± 186.17 | ||