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Purification of Murine Gammaherpesvirus 68 With Use of Differential Centrifugation Cover

Purification of Murine Gammaherpesvirus 68 With Use of Differential Centrifugation

European Pharmaceutical Journal
Volume 68 (2021): Issue 1 (January 2021)
By: R. Hodoši,  E. Nováková and  M. Šupolíková  
Open Access
|Jul 2021

Figures & Tables

Figure 1

Diagram of particle behaviour during differential centrifugation and density gradient centrifugation. a) During the differential centrifugation of a suspension of particles in a centrifugal field, the movement of the particles depends on their density, shape and size. b) To separate the biological particles by means of a density gradient, the samples are layered on the prepared gradient before centrifugation. To achieve separation, the sample is centrifuged, until the isopycnic position for the desired particles in the gradient is achieved.
Diagram of particle behaviour during differential centrifugation and density gradient centrifugation. a) During the differential centrifugation of a suspension of particles in a centrifugal field, the movement of the particles depends on their density, shape and size. b) To separate the biological particles by means of a density gradient, the samples are layered on the prepared gradient before centrifugation. To achieve separation, the sample is centrifuged, until the isopycnic position for the desired particles in the gradient is achieved.

Figure 2

Schematic diagram of virus purification steps.
Schematic diagram of virus purification steps.

Figure 3

Polypeptide profile of purified MHV-68 in gradient SDS-PAGE (6%–14%, w/v). Lane 1: ladder (Precision Plus Protein™ Dual Xtra); lane 2: purified MHV-68 (centrifuged 6 hours) – 10 μl/lane; lane 3: purified MHV-68 (centrifuged 6 hours) – 5 μl/lane; lane 4: purified MHV-68 (centrifuged 6 hours) – 3 μl/lane.
Polypeptide profile of purified MHV-68 in gradient SDS-PAGE (6%–14%, w/v). Lane 1: ladder (Precision Plus Protein™ Dual Xtra); lane 2: purified MHV-68 (centrifuged 6 hours) – 10 μl/lane; lane 3: purified MHV-68 (centrifuged 6 hours) – 5 μl/lane; lane 4: purified MHV-68 (centrifuged 6 hours) – 3 μl/lane.

Figure 4

Molecular weights (in kDa) of viral proteins of purified MHV-68, gradient SDS-PAGE (6%–14% w/v). Lane 1: ladder (Precision Plus Protein™ Dual Xtra); lane 2: purified MHV-68 (centrifuged 6 hours) – 12 μl/lane; lane 3: purified MHV-68 (centrifuged 6 hours) – 6 μl/lane.
Molecular weights (in kDa) of viral proteins of purified MHV-68, gradient SDS-PAGE (6%–14% w/v). Lane 1: ladder (Precision Plus Protein™ Dual Xtra); lane 2: purified MHV-68 (centrifuged 6 hours) – 12 μl/lane; lane 3: purified MHV-68 (centrifuged 6 hours) – 6 μl/lane.
DOI: https://doi.org/10.2478/afpuc-2021-0009 | Journal eISSN: 2453-6725
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Language: English
Page range: 76 - 79
Submitted on: Jun 14, 2021
|
Accepted on: Jun 17, 2021
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Published on: Jul 30, 2021
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
MHV-68,
purification,
virus proteins,
SDS-PAGE
Related subjects:
Pharmacy,
Pharmacy, other

© 2021 R. Hodoši, E. Nováková, M. Šupolíková, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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