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Rapid and simple CZE-UV method for quality control of B1 and B6 vitamins in drugs and dietary supplements Cover

Rapid and simple CZE-UV method for quality control of B1 and B6 vitamins in drugs and dietary supplements

European Pharmaceutical Journal
Volume 68 (2021): Issue 1 (January 2021)
By: M. Matuskova,  I. Cizmarova,  P. Chalova,  P. Mikus and  J. Piestansky  
Open Access
|Jul 2021

Figures & Tables

Figure 1

Analysis of THI and PYR in various pharmaceutical dosage forms – a) injection solutions Milgamma NA, b) film-coated tablets B-komplex Sanofi and c) capsules Diclovit. d) Illustrative electropherogram of THI and PYR at the concentration level 0.25 μg/mL. e) Illustrative electropherogram obtained from the CZE-UV analysis of non-spiked and spiked drug Diclovit. The spiked concentrations of THI and PYR were 5, 10 and 25 μg/mL. The injected volume was 200 nL.
Analysis of THI and PYR in various pharmaceutical dosage forms – a) injection solutions Milgamma NA, b) film-coated tablets B-komplex Sanofi and c) capsules Diclovit. d) Illustrative electropherogram of THI and PYR at the concentration level 0.25 μg/mL. e) Illustrative electropherogram obtained from the CZE-UV analysis of non-spiked and spiked drug Diclovit. The spiked concentrations of THI and PYR were 5, 10 and 25 μg/mL. The injected volume was 200 nL.

THI and PYR concentrations in three pharmaceutical samples determined by the CZE-UV method_

Preparation Parameters
Found ± SD (μg/mL)RSD (%), n=3Declared (μg/mL)
Milgamma NA (inj)THI53.15 ± 0.260.4950
PYR23.01 ± 0.230.9825
Diclovit (cps)THI50.81 ± 1.272.5050
PYR43.18 ± 1.072.4850
B-komplex Sanofi (tbl flm)THI11.59 ± 0.181.5610
PYR5.52 ± 0.509.005

Performance parameters of the CZE-UV method_

ThiaminePyridoxine
tm (min)7.0111.02
RSDtm (%), n = 60.280.76
RSDarea (%), n = 62.288.60
a (mAU)134.76−39.09
RSDa (%), n = 62.271.33
b (mAU/μg mL)94.7736.22
RSDb (%), n = 60.490.29
r20.99960.9990
Linear range (μg/mL)0.5–1001–100
LOD (μg/mL)0.080.15
LOQ (μg/mL)0.250.50
N79006400
R8.81
DOI: https://doi.org/10.2478/afpuc-2021-0002 | Journal eISSN: 2453-6725
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Language: English
Page range: 59 - 62
Submitted on: Jun 15, 2021
Accepted on: Jun 17, 2021
Published on: Jul 25, 2021
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
capillary zone electrophoresis,
hydrodynamically closed system,
ultraviolet detection,
thiamine,
pyridoxine,
quality control
Related subjects:
Pharmacy,
Pharmacy, other

© 2021 M. Matuskova, I. Cizmarova, P. Chalova, P. Mikus, J. Piestansky, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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