Figure 1
Dependence of normalised intensity of scattered light (INORM) on the concentration of added DDAO (cDDAO), both types of membrane at a lipid concentration of 0.1 mmol/dm3. Inset shows a comparison of the experiment at three different concentrations of POPE-POPG-TOCL (intensity (I) was not normalised).
Figure 2
Dependence of scattered light intensity (I) on the concentration of added DDAO, cPOPE+POPG+TOCL = 0.06 mmol/dm3. Arrows indicate the critical concentrations DtSAT, DtSOL acquired using bilinear functions (full line) and DtMID using reverse sigmoid function (dashed line).
Figure 3
Linear dependencies of critical DDAO concentrations on the concentration of POPE-POPG (left) and POPE-POPG-TOCL (right). The figure shows the global fit used to calculate the effective molar ratios (Re ) and the partition coefficients (Kp). DwSAT represents the concentration of DDAO surfactant in the water phase at the stage of complete saturation of the lipid bilayers by DDAO.
Table 1
Global fit (Equation 3) results of POPE-POPG and POPE-POPG-TOCL static light scattering data.
| Critical DDAO concentration | POPE-POPG | POPE-POPG-TOCL | ||
|---|---|---|---|---|
| Re | DW [mmol/dm3] | Re | DW [mmol/dm3] | |
| D tSAT | 3.7 ± 0.2 | 0.9 ± 0.1 | 5.0 ± 0.3 | 1.0 ± 0.1 |
| DtMID | 4.6 ± 0.3 | 1.2 ± 0.2 | 6.3 ± 0.3 | 1.2 ± 0.2 |
| DtSOL | 5.1 ± 0.3 | 1.3 ± 0.2 | 7.5 ± 0.4 | 1.4 ± 0.2 |
| Kp | 5,300 ± 400 | 6,500 ± 500 | ||
[i] Re is the effective ratio of the amount of DDAO integrated into the lipid bilayer to the amount of lipid. DW is the concentration of surfactant in the water phase. Kp is molar partition coefficient of DDAO between the bilayer and water phase.