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Study of the solubilisation process of bacterial model membranes induced by DDAO Cover

Study of the solubilisation process of bacterial model membranes induced by DDAO

European Pharmaceutical Journal
Volume 68 (2021): Issue 1 (January 2021)
By: K. Želinská and  J. Gallová  
Open Access
|May 2021

Figures & Tables

Figure 1

Dependence of normalised intensity of scattered light (INORM) on the concentration of added DDAO (cDDAO), both types of membrane at a lipid concentration of 0.1 mmol/dm3. Inset shows a comparison of the experiment at three different concentrations of POPE-POPG-TOCL (intensity (I) was not normalised).
Dependence of normalised intensity of scattered light (INORM) on the concentration of added DDAO (cDDAO), both types of membrane at a lipid concentration of 0.1 mmol/dm3. Inset shows a comparison of the experiment at three different concentrations of POPE-POPG-TOCL (intensity (I) was not normalised).

Figure 2

Dependence of scattered light intensity (I) on the concentration of added DDAO, cPOPE+POPG+TOCL = 0.06 mmol/dm3. Arrows indicate the critical concentrations DtSAT, DtSOL acquired using bilinear functions (full line) and DtMID using reverse sigmoid function (dashed line).
Dependence of scattered light intensity (I) on the concentration of added DDAO, cPOPE+POPG+TOCL = 0.06 mmol/dm3. Arrows indicate the critical concentrations DtSAT, DtSOL acquired using bilinear functions (full line) and DtMID using reverse sigmoid function (dashed line).

Figure 3

Linear dependencies of critical DDAO concentrations on the concentration of POPE-POPG (left) and POPE-POPG-TOCL (right). The figure shows the global fit used to calculate the effective molar ratios (Re ) and the partition coefficients (Kp). DwSAT represents the concentration of DDAO surfactant in the water phase at the stage of complete saturation of the lipid bilayers by DDAO.
Linear dependencies of critical DDAO concentrations on the concentration of POPE-POPG (left) and POPE-POPG-TOCL (right). The figure shows the global fit used to calculate the effective molar ratios (Re ) and the partition coefficients (Kp). DwSAT represents the concentration of DDAO surfactant in the water phase at the stage of complete saturation of the lipid bilayers by DDAO.

Global fit (Equation 3) results of POPE-POPG and POPE-POPG-TOCL static light scattering data_

Critical DDAO concentrationPOPE-POPGPOPE-POPG-TOCL
ReDW [mmol/dm3]ReDW [mmol/dm3]
D tSAT3.7 ± 0.20.9 ± 0.15.0 ± 0.31.0 ± 0.1
DtMID4.6 ± 0.31.2 ± 0.26.3 ± 0.31.2 ± 0.2
DtSOL5.1 ± 0.31.3 ± 0.27.5 ± 0.41.4 ± 0.2
Kp5,300 ± 4006,500 ± 500
DOI: https://doi.org/10.2478/afpuc-2020-0019 | Journal eISSN: 2453-6725
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Language: English
Page range: 17 - 23
Submitted on: Nov 30, 2020
|
Accepted on: Mar 1, 2021
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Published on: May 11, 2021
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
N,N-dimethyl-1-dodecanamine-N-oxide,
solubilisation,
bacterial model membranes,
cardiolipin
Related subjects:
Pharmacy,
Pharmacy, other

© 2021 K. Želinská, J. Gallová, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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