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Concentration-dependent effect of silymarin on concanavalin A-stimulated mouse spleen cells in vitro Cover

Concentration-dependent effect of silymarin on concanavalin A-stimulated mouse spleen cells in vitro

European Pharmaceutical Journal
Volume 67 (2020): Issue 1 (January 2020)
By: G. Hrčková,  T. Mačák Kubašková,  D. Mudroňová and  A. Bardelčíková  
Open Access
|Nov 2020

Figures & Tables

Figure 1

The effect of silymarin on CoA-activated mouse spleen cells in vitro: (a) proliferation index of T lymphocytes; (b) viability test of T lymphocytes using trypan blue staining. Significantly different values between CoA alone and CoA + silymarin treated groups are indicated as *p < 0.05, **p < 0.01, ***p < 0.001.
The effect of silymarin on CoA-activated mouse spleen cells in vitro: (a) proliferation index of T lymphocytes; (b) viability test of T lymphocytes using trypan blue staining. Significantly different values between CoA alone and CoA + silymarin treated groups are indicated as *p < 0.05, **p < 0.01, ***p < 0.001.

Figure 2

The effect of silymarin on cytokine secretion and mRNA levels in control unstimulated splenocytes and CoA-activated mouse spleen T lymphocytes in vitro: (a) concentration of IFN-γ and (b) IL-4 in supernatants of cells after 70 h cultivation; (c) relative gene expression for IFN-γ and (d) IL-4 in T lymphocytes after 70 h of cultivation. Significantly different values are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001.
The effect of silymarin on cytokine secretion and mRNA levels in control unstimulated splenocytes and CoA-activated mouse spleen T lymphocytes in vitro: (a) concentration of IFN-γ and (b) IL-4 in supernatants of cells after 70 h cultivation; (c) relative gene expression for IFN-γ and (d) IL-4 in T lymphocytes after 70 h of cultivation. Significantly different values are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001.

Figure 3

The effect of silymarin on mRNA levels in control unstimulated splenocytes and CoA-activated mouse spleen T lymphocytes in vitro for (a) transcription factor NF-κB and (b) transcription factor Foxp3. Significantly different values are indicated as *p < 0.05, **p < 0.01, ***p < 0.001.
The effect of silymarin on mRNA levels in control unstimulated splenocytes and CoA-activated mouse spleen T lymphocytes in vitro for (a) transcription factor NF-κB and (b) transcription factor Foxp3. Significantly different values are indicated as *p < 0.05, **p < 0.01, ***p < 0.001.

Figure 4

The effect of silymarin on mitochondrial membrane potential in naïve and CoA-activated mouse spleen cells and after treatment with silymarin (b), expressed as the mean intensity of fluorescence (MIF) for Rhodamine 123: (a) representative plots of MIF for control and individual silymarin-treated groups. Significantly different values are indicated as: *p < 0.05.
The effect of silymarin on mitochondrial membrane potential in naïve and CoA-activated mouse spleen cells and after treatment with silymarin (b), expressed as the mean intensity of fluorescence (MIF) for Rhodamine 123: (a) representative plots of MIF for control and individual silymarin-treated groups. Significantly different values are indicated as: *p < 0.05.

Figure 5

The proportions of live, early apoptotic, and late apoptotic/dead cells determined for the suspensions of naïve splenocytes, CoA-activated T lymphocytes, and following in vitro treatment with silymarin after 70 h of cultivation (b). Representative flow cytometric dot plots showing Annexin V and propidium iodide staining of naïve splenocytes and CoA-activated cells following silymarin treatment (a). Significantly different values between CoA alone and silymarin-treated groups for early apoptotic cells are indicated as *p < 0.05 and for live cells are indicated as Ñp < 0.05.
The proportions of live, early apoptotic, and late apoptotic/dead cells determined for the suspensions of naïve splenocytes, CoA-activated T lymphocytes, and following in vitro treatment with silymarin after 70 h of cultivation (b). Representative flow cytometric dot plots showing Annexin V and propidium iodide staining of naïve splenocytes and CoA-activated cells following silymarin treatment (a). Significantly different values between CoA alone and silymarin-treated groups for early apoptotic cells are indicated as *p < 0.05 and for live cells are indicated as Ñp < 0.05.
DOI: https://doi.org/10.2478/afpuc-2020-0003 | Journal eISSN: 2453-6725
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Language: English
Page range: 17 - 26
Submitted on: Oct 2, 2019
Accepted on: Dec 10, 2019
Published on: Nov 18, 2020
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
silymarin,
mouse,
splenocytes,
proliferation,
mitochondrial potential,
apoptosis
Related subjects:
Pharmacy,
Pharmacy, other

© 2020 G. Hrčková, T. Mačák Kubašková, D. Mudroňová, A. Bardelčíková, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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