Exploration of Novel Target Via Genome Mining and Establishment of Proofman-LMTIA Assay for the Rapid Detection of Chlamydia abortus
References
- Marti H, Jelocnik M: Animal Chlamydiae: A Concern for Human and Veterinary Medicine. Pathogens. 2022, 11(3):364.
- Chaiwattanarungruengpaisan S, Thongdee M, Arya N, Paungpin W, Sirimanapong W, Sariya L: Diversity and genetic characterization of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis). Acta Trop 2024, 253:107183.
- Chaiwattanarungruengpaisan S, Thongdee M, Anuntakarun S, Payungporn S, Arya N, Punchukrang A, Ramasoota P, Singhakaew S, Atithep T, Sariya L: A new species of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis). PLoS One 2021, 16(5):e0252081.
- Li C, Gao J, Shi S, Gao W, Zhu X: The Seroprevalence of Chlamydia Infection in Sheep in Shanxi Province, China. Vaccines (Basel) 2022, 9(12):656.
- Turin L, Surini S: Recent advances and public health implications for environmental exposure to Chlamydia abortus: from enzootic to zoonotic disease. Vet Res 2022, 53(1):37.
- Ali A, Derar DR, Mousa HA, Osman SA, Refaai W, Almundarij TI, Al-Dubaib MA, Allam SA: First report on the isolation of Chlamydia abortus from female dromedary camels with ovarian hydrobursitis. Theriogenology 2022, 191:102-108.
- Meijer A, Brandenburg A, de Vries J, Beentjes J, Roholl P, Dercksen D: Chlamydophila abortus infection in a pregnant woman associated with indirect contact with infected goats. Eur J Clin Microbiol Infect Dis 2004, 23(6):487-490.
- Essig A, Longbottom D: Chlamydia abortus: New aspects of infectious abortion in sheep and potential risk for pregnant women. Curr Clin Microbiol 2015, 2(1):22-34.
- Burgener AV, Seth-Smith HMB, Kern-Baumann S, Durovic A, Blaich A, Menter T, Bruder E, Roloff T, Martinez A, Borel N, Albini S, Hösli I, Egli A, Weisser M, Hinić V: A case study of zoonotic Chlamydia abortus infection: diagnostic challenges from clinical and microbiological perspectives. Open Forum Infect Dis 2022, 9(10):ofac524.
- Chisu V, Zobba R, Masala G, Tran TL, Ngo Viet QT, Tran DB, Nguyen HB, Tran KT, Alberti A: First molecular detection of zoonotic Chlamydia species in Vietnamese goats. Pathogens 2022, 11 (8):903.
- Longbottom D, Livingstone M, Maley S, van der Zon A, Rocchi M, Wilson K, Wheelhouse N, Dagleish M, Aitchison K, Wattegedera S, Nath M, Entrican G, Buxton D: Intranasal infection with Chlamydia abortus induces dose-dependent latency and abortion in sheep. PLoS One 2013, 8(2): e57950.
- Rodolakis A, Laroucau K: Chlamydiaceae and chlamydial infections in sheep or goats. Vet Microbiol 2015, 181(1-2):107-118.
- Longbottom D, Sait M, Livingstone M, Laroucau K, Sachse K, Harris SR, Thomson NR, Seth-Smith HMB: Genomic evidence that the live Chlamydia abortus vaccine strain 1B is not attenuated and has the potential to cause disease. Vaccine (Basel) 2018, 36(25):3593-3598.
- Livingstone M, Wattegedera SR, Palarea-Albaladejo J, Aitchison K, Corbett C, Sait M, Wilson K, Chianini F, Rocchi MS, Wheelhouse N, Entrican G, Longbottom D: Efficacy of Two Chlamydia abortus Subcellular Vaccines in a Pregnant Ewe Challenge Model for Ovine Enzootic Abortion. Vaccines (Basel) 2021, 9(8):898.
- Barati S, Bakhtiarim NM, Shokoohizadeh L, Ghorbanpoor M, Momtaz H: Genotyping of Chlamydia abortus using multiple loci variable number of tandem repeats analysis technique. BMC Vet Res 2022, 18(1):54.
- Wang D, Wang Y, Zhang M, Zhang Y, Sun J, Song C, Xiao F, Ping Y, Pan C, Hu Y, Wang C, Liu Y: ladder-shape melting temperature isothermal amplification of nucleic acids. Biotechniques 2021, 71(1):358-369.
- Wang Y, Wang B, Xu D, Zhang M, Zhang X, Wang D: Development of a ladder-shape melting temperature isothermal amplification (LMTIA) assay for detection of African swine fever virus (ASFV). J Vet Sci 2022, 23(4):e51.
- Gu M, Xiao F, Wang B, Zhang Y, Ding C, Zhang G, Wang D: Study on detection of soybean components in edible oil with ladder-shape melting temperature isothermal amplification (LMTIA) assay. Anal Methods 2023, 15(5):581-586.
- Song C, Wang B, Wang Y, Liu J, Wang D: Detection of Listeria monocytogenes in food using the Proofman-LMTIA assay. Molecules 2023, 28(14):5457.
- Xiao F, Gu M, Zhang Y, Xian Y, Zheng Y, Zhang Y, Sun J, Ding C, Zhang G, Wang D: Detection of soybean-derived components in dairy products using proofreading enzyme-mediated probe cleavage coupled with ladder-shape melting temperature isothermal amplification (Proofman-LMTIA). Molecules 2023, 28(4):1685.
- Zhang X, Li Z, Zhang Y, Xu D, Zhang L, Xiao F, Wang D: Rapid discrimination of Panax quinquefolium and Panax ginseng using the Proofman-Duplex-LMTIA technique. Molecules 2023, 28(19):6872.
- Cui P, Hu Z, Guo M, Wang Y, Xu D, Yao W, Liu J, Song C, Sun J, Xiao F, Wang D: Rapid and duplex detection of sweetpotato and maize components in starch products using Proofman-LMTIA method. Microchem J 2024, 204:111082-111082.
- Liu J, Wang Y, Li T, Huang K, Song C, Cui P, Yin B, Sun J, Xiao F, Wang D: Dual detection of Bupleurum scorzonerifolium Willd. and Bupleurum chinense DC. using proofman– LMTIA method. Chem Biol Technol Ag 2024, 11(1):104-104.
- Ma D, Deng L, Huang L, Peng A, Bi W, Li R: Genome mining of novel targets and construction of Ladder-shaped melting temperature isothermal amplification assays for the identification of Cronobacter sakazakii and Cronobacter malonaticus. Foodborne Pathog Dis 2024, 21(9):586-592.
- Xiao F, Zhang Y, Gu M, Xi X, Zhang Y, Sun J, Wei Q, Hu B, Zhang G, Wang D: Comparative study on the target sequences of thaumatin-like protein (TLP) and internal transcribed spacer (ITS) in the detection of corn component in edible oil by ladder-shape melting temperature isothermal amplification (LMTIA). LWT-food Sci Technol 2024, 197:115903.
- Zhou J, Li Z, Lou Z, Fei Y: Prevalence, diagnosis, and vaccination situation of animal chlamydiosis in China. Front Vet Sci 2018, 5:88.
- Burnard D, Polkinghorne A: Chlamydial infections in wildlife-conservation threats and/or reservoirs of ‘spill-over’ infections? Vet Microbiol 2016, 196:78-84.
- Borel N, Polkinghorne, Pospischil A: A review on chlamydial diseases in animals: still a challenge for pathologists? Vet Pathol 2018, 55(3):374-390.
- Manual OT: Office International des Epizooties. Chapter 3.7.5. – Enzootic abortion of ewes (ovine chlamydiosis). In: Manual of diagnostic tests and vaccines for terrestrial animals. World Organisation for Animal Health 2018, 1456–1465.
- O’Neill LM, O’Driscoll Á, Markey B: Comparison of three commercial serological tests for the detection of Chlamydia abortus infection in ewes. Ir Vet J 2018, 71:13.
- Wilson K, Livingstone M, Longbottom D: Comparative evaluation of eight serological assays for diagnosing Chlamydophila abortus infection in sheep. Vet Microbiol 2009, 135(1-2):38-45.
- Buendía AJ, Cuello F, Del Rio L, Gallego MC, Caro MR, Salinas J: Field evaluation of a new commercially available ELISA based on a recombinant antigen for diagnosing Chlamydophila abortus (Chlamydia psittaci serotype 1) infection. Vet Microbiol 2001, 78(3):229-239.
- Livingstone M, Entrican G, Wattegedera S, Buxton D, McKendrick IJ, Longbottom D: Antibody responses to recombinant protein fragments of the major outer membrane protein and polymorphic outer membrane protein POMP90 in Chlamydophila abortus-infected pregnant sheep. Clin Diagn Lab Immunol 2005, 12(6):770-777.
- Longbottom D, Psarrou E, Livingstone M, Vretou E: Diagnosis of ovine enzootic abortion using an indirect ELISA (rOMP91B iELISA) based on a recombinant protein fragment of the polymorphic outer membrane protein POMP91B of Chlamydophila abortus. FEMS Microbiol Lett 2001, 195(2):157-161.
- McCauley LM, Lancaster MJ, Young P, Butler KL, Ainsworth CG: Comparison of ELISA and CFT assays for Chlamydophila abortus antibodies in ovine sera. Aust Vet J 2007, 85(8):325-328.
- Pantchev A, Sting R, Bauerfeind R, Tyczka J, Sachse K: New real-time PCR tests for species-specific detection of Chlamydophila psittaci and Chlamydophila abortus from tissue samples. Vet J 2009, 181(2):145-150.
- Krõlov K, Frolova J, Tudoran O, Suhorutsenko J, Lehto T, Sibul H, Mäger I, Laanpere M, Tulp I, Langel Ü: Sensitive and rapid detection of Chlamydia trachomatis by recombinase polymerase amplification directly from urine samples. J Mol Diagn 2014, 16(1):127-35.
- Hulse LS, McDonald S, Johnston SD, Beagley KW: Rapid point-of-care diagnostics for the detection of Chlamydia pecorum in koalas (Phascolarctos cinereus) using loop-mediated isothermal amplification without nucleic acid purification. Microbiologyopen 2019, 8(12):e916.
- Somboonna N, Choopara I: Point-of-care Chlamydia trachomatis detection using loop-mediated isothermal amplification and hydroxynaphthol blue. Methods Mol Biol 2019, 2042:11-17.
Language: English
Page range: 159 - 172
Submitted on: Jan 5, 2026
Accepted on: May 14, 2026
Published on: Jun 23, 2026
Published by: University of Belgrade, Faculty of Veterinary Medicine
In partnership with: Paradigm Publishing Services
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© 2026 Zhaohui Cui, Yahang Han, Yanan Zhang, Jiayi Wang, Weidong Zhang, Xiyao Huang, Junqiang Li, Deguo Wang, published by University of Belgrade, Faculty of Veterinary Medicine
This work is licensed under the Creative Commons Attribution 4.0 License.