Have a personal or library account? Click to login
Occurrence, Enterotoxigenic Profile, and Antimicrobial Resistance of Staphylococcus aureus Isolates from Bovine Mastitis Milk Samples Cover

Occurrence, Enterotoxigenic Profile, and Antimicrobial Resistance of Staphylococcus aureus Isolates from Bovine Mastitis Milk Samples

Open Access
|Apr 2026

References

  1. Savini, Vincenzo: Pet-to-man travelling staphylococci – A world in progress (coauthored book – Editor: Vincenzo Savini – London, United Kingdom; Elsevier/Academic Press, 2018. Print.
  2. Alagić D, Carpenter TE, Thurmond MC: Partial Budget Analysis for Vaccination against Clinical Coliform Mastitis on a Bosnian Dairy. Proceedings, 11th International Symposium on Veterinary Epidemiology and Economics, Cairns, Australia, Theme 5 – Evaluation of animal disease: Economics session 5.2.6, 2006, p 706.
  3. Alagić D, Smajlović M, Članjak E, Čaklovica K: Staphylococcus aureus – patogen prenosiv hranom, Veterinarski fakultet, Univerziteta u Sarajevu, 2015, p 72; ISBN : 978-9958-599-58-3 (in Bosnian language); 2015, 72-75.
  4. Deng Z, Koop G, Hogeveen H, Fischer EAJ, van den Borne BHP, van der Tol R, Lam TJ GM: Transmission dynamics of Staphylococcus aureus and Streptococcus agalactiae in a Dutch dairy herd using an automatic milking system. Prev Vet Med 2021, 192:105384.
  5. Sukalić T, Đuričić D, Pavljak I, Končurat A, Cvetnić Ž, Grbavac J, Bačanek B, Jurmanović J, Samardžija M: Antimikrobna osjetljivost uzročnika mastitisa krava s područja sjeverozapadne Hrvatske u razdoblju od 2014. do 2018. godine, Vet Stn 2021, 52 (2):147-156.
  6. Rifatbegović M, Nicholas RAJ . Mutevelić T, Hadžiomerović M, Maksimović Z: Pathogens associated with bovine mastitis: the experience of Bosnia and Herzegovina. Vet Sci 2024, 11:63.
  7. Haag AF, Fitzgerald JR, Penadés JR : Staphylococcus aureus in Animals. Microbiol Spectr 2019, 7(3):10.1128.
  8. Grispoldi L, Massetti L, Sechi P, Iulietto MF, Ceccarelli M, Karama M, Popescu PA, Pandolfi F, Cenci-Goga BT : Short communication: Characterization of enterotoxin-producing S. aureus isolated from mastitic cows. J Dairy Sci 2019, 102(2):1059-1065.
  9. Fursova KK., Shchannikova MP, Loskutova IV, Shepelyakovskaya AO, Laman AG, Boutanaev AM, Sokolov SL., Artem’eva OA, Nikanova DA, Zinovieva, Brovko FA: Exotoxin diversity of Staphylococcus aureus isolated from milk of cows with subclinical mastitis in Central Russia. J Dairy Sci 2018, 101(5):4325–4331.
  10. 1Krömker V, Leimbach S: Mastitis treatment— Reduction in antibiotic usage in dairy cows. Reprod Dom Anim 2017, 52(Suppl. 3):21-29.
  11. WHO: World Health Organization. Food Safety, 2024. [https://www.who.int/news-room/fact-sheets/detail/food-safety].
  12. Kernodle DS: Mechanisms of resistance to β-lactam antibiotics. In: Gram-Positive Pathogens 2006, 769-781.
  13. Kaur DC, Chate SS: Study of antibiotic resistance pattern in methicillin resistant Staphylococcus aureus with special reference to newer antibiotic. J Glob Infect Dis 2015, 7(2):78-84.
  14. CLSI: Clinical and Laboratory Standard Institute. CLSI Guideline M100 – Performance Standards for Antimicrobial Disk Susceptibility Tests (33th edn). Wayne, PA: CLSI, 2023 [https://clsi.org/].
  15. Cvetnić L, Samardžija M, Duvnjak S, Habrun B, Cvetnić M, Jaki Tkalec V, Đuričić D, Benić M: Multi locus sequence typing and spa typing of Staphylococcus aureus isolated from the milk of cows with subclinical mastitis in Croatia. Microorganisms 2021, 9(4):725.
  16. Firdaus, I. Laboratory Handbook on Bovine Mastitis. National Mastitis Council (NMC). Available Online February 2017, [https://www.academia.edu/50651208/Laboratory_Handbook_on_Bovine_Mastitis].
  17. Fetsch A. (2017). Staphylococcus aureus 1st Edition. Publisher: Academic Press. Publication date: September 26, 2017. [eBook ISBN : 9780128097977 Paperback ISBN : 9780128096710].
  18. DTU National Food Institute – EU Reference Laboratory Antimicrobial resistance: Isolation of methicillin-resistant Staphylococcus aureus (MRSA) from food-producing animals and farm environment. Available Online (Accessed on May 22, 2023), 2023. [https://www.food.dtu.dk/english//media/institutter/foedevareinstituttet/temaer/antibiotikaresistens/eurl-ar/protocols/mrsa/2_716_mrsa-pcr-2-v3-1.pdf].
  19. Metoda 01M.PCR, Institut za higijenu i tehnologiju mesa AT S 01-049. Belgrade, Serbia
  20. ISO 19020:2017: Microbiology of the food chain — Horizontal method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs. Published (Edition 1, 2017). This publication was last reviewed and confirmed in 2022. Therefore, this version remains current, 2022, [https://www.iso.org/standard/63747.html].
  21. IBM SPSS Statistics 20. Statistical Package for the Social Sciences. [https://www.ibm.com/support/pages/downloading-ibm-spss-statistics-20].
  22. Zigo F, Farkašová Z, Výrostková J, Regecová I, Ondrašovičová S, Vargová M, Sasáková N, Pecka-Kielb E, Bursová Š, Kiss DS: Dairy Cows’ Udder Pathogens and Occurrence of Virulence Factors in Staphylococci. Animals 2022, 12(4):470.
  23. Vujinović S, Graber HU, Vićić I, Vejnović B, Stevanović O, Krnjaić D, Milivojević D, Katić, V: Genotypes and virulence factors in Staphylococcus aureus isolated from dairy cows with subclinical mastitis in Serbia. Comp Immunol Microbiol Infect Dis 2023, 101:102056.
  24. Büthe T, Jessberger N, Schneider B, Kreienbrock L, Plötz M: Occurrence of mastitis pathogens in cow’s milk samples from the Hanover region, north-western Germany – an overview of routine laboratory data from 2005 to 2023. Vet Res Commun 2025, 49(4):208.
  25. Smistad M, Bakka HC, Sølverød L, Jørgensen HJ, Wolff C: Prevalence of udder pathogens in milk samples from Norwegian dairy cows recorded in a national database in 2019 and 2020. Acta Vet Scand 2023, 65(1):19.
  26. Dobrut A, Siemińska I, Sroka-Oleksiak A, Drożdż K, Sobońska J, Mroczkowska U, Brzychczy-Włoch M: Molecular and phenotypic identification of bacterial species isolated from cows with mastitis from three regions of Poland. BMC Vet Res 2024, 20(1):193
  27. Naranjo-Lucena A, Becker P, Madigan G, Cupial R, Byrne B, Johnson A: Longitudinal patterns in the isolation and antimicrobial resistance of bovine mastitis-causing bacteria in Ireland. Antibiotics 2025, 14(3):243.
  28. Fang R, Cui J, Cui T, Guo H, Ono HK, Park C-H, Okamura M, Nakane A, Hu DL : Staphylococcal Enterotoxin C Is an important virulence factor for mastitis. Toxins 2019, 11(3):141.
  29. Pajić M, Boboš S, Velebit B, Rašić Z, Katić V, Radinović M, Nikolić A, Simonović D, Babić M: Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows. Acta Vet-Beograd 2016, 66(4):466-477.
  30. Boynukara B, Gulhan T, Alisarli M, Gurturk K, Solmaz H: Classical enterotoxigenic characteristics of Staphylococcus aureus strains isolated from bovine subclinical mastitis in Van, Turkey. Int J Food Microbiol 2008, 125(2):209-211.
  31. Zaatout N, Hezil D: A meta-analysis of the global prevalence of methicillin-resistant Staphylococcus aureus (MRSA) isolated from clinical and subclinical bovine mastitis. J Appl Microbiol 2022, 132(1):140-154
  32. Paterson GK, Morgan FJ, Harrison EM, Peacock SJ, Parkhill J, Zadoks RN, Holmes MA: Prevalence and properties of mecC methicillin-resistant Staphylococcus aureus (MRSA) in bovine bulk tank milk in Great Britain. J Antimicrob Chemother 2014, 69(3):598-602.
  33. Zigo F, Farkašová Z, Výrostková J, Regecová I, Ondrašovičová S, Vargová M, Sasáková N, Pecka-Kielb E, Bursová Š, Kiss DS: Dairy Cows’ Udder Pathogens and Occurrence of Virulence Factors in Staphylococci. Animals 2022, 12(4):470
  34. Suleiman AS, Bhattacharya P, Islam MA: Global prevalence and dynamics of mecA and mecC genes in MRSA: Meta-meta-analysis, meta-regression, and temporal investigation. J Infect Public Health. 2025, 18(7):102802.
  35. Dierikx C, Hengeveld P, Witteveen S, van Hoek A, van Santen-Verheuvel M, Montizaan M, Kik M, Maas M, Schouls L, Hendrickx A, Dutch MRSA surveillance study group & van Duijkeren: Genomic comparison of mecC-carrying methicillin-resistant Staphylococcus aureus from hedgehogs and humans in the Netherlands. J Antimicrob Chemother 2023, 78(5):1168-1174.
  36. Nemati G, Romanó A, Wahl F, Berger T, Rojo LV and Graber HU: Bovine Staphylococcus aureus: a European study of contagiousness and antimicrobial resistance. Front Vet Sci 2023, 10:1154550.
DOI: https://doi.org/10.2478/acve-2026-0004 | Journal eISSN: 1820-7448 | Journal ISSN: 0567-8315
Language: English
Page range: 53 - 65
Submitted on: Dec 4, 2025
|
Accepted on: Mar 2, 2026
|
Published on: Apr 2, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Related subjects:

© 2026 Tanja Ilić, Enida Članjak-Kudra, Dragan Knežević, Branko Velebit, Oliver Stevanović, Nataša Kilibarda, Davor Alagić, published by University of Belgrade, Faculty of Veterinary Medicine
This work is licensed under the Creative Commons Attribution 4.0 License.