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Glycosylation Changes in Dairy Calf IGG Heavy Chains during Pre-Ruminant to Ruminant Transition: Pilot Study Cover

Glycosylation Changes in Dairy Calf IGG Heavy Chains during Pre-Ruminant to Ruminant Transition: Pilot Study

Open Access
|Dec 2025

References

  1. Butler JE: Bovine immunoglobulins: an augmented review. Vet Immunol Immunopathol 1983, 4(1-2):43-152.
  2. Zhao Y, Jackson SM, Aitken R: The bovine antibody repertoire. Dev Comp Immunol 2006, 30:175-186.
  3. Vlasova AN, Saif LJ: Bovine Immunology: Implications for Dairy Cattle. Front Immunol 2021, 12:643206.
  4. Fuji S, Nishiura T, Nishikawa A, Miura R, Taniguchi N: Structural heterogeneity of sugar chains in immunoglobulin G. Conformation of immunoglobulin G molecule and substrate specificities of glycosyltransferases. J Biol Chem 1990, 265(11): 6009-6018.
  5. Raju TS, Briggs JB, Borge SM, Jones AJ: Species-specific variation in glycosylation of IgG: evidence for the species-specific sialylation and branch-specific galactosylation and importance for engineering recombinant glycoprotein therapeutics. Glycobiology 2000, 10(5):477-486.
  6. Saba JA, Kunkel JP, Jan DC, Ens WE, Standing KG, Butler M, Jamieson JC, Perreault H: A study of immunoglobulin G glycosylation in monoclonal and polyclonal species by electrospray and matrix-assisted laser desorption/ionization mass spectrometry. Anal Biochem 2002, 305(1):16-31.
  7. Adamczyk B, Tharmalingam-Jaikaran T, Schomberg M, Szekrényes Á, Kelly RM, Karlsson NG, Guttman A, Rudd PM: Comparison of separation techniques for the elucidation of IgG N-glycans pooled from healthy mammalian species. Carbohydr Res 2014, 389:174-185.
  8. Takimori S, Shimaoka H, Furukawa J, Yamashita T, Amano M, Fujitani N, Takgawa Y, Hammarström L, Kacskovics I, Shinohara Y, Nishimura S. Alteration of the N-glycome of bovine milk glycoproteins during early lactation. FEBS J 2011, 278(19): 3769-3781.
  9. Feeney S, Gerlach JQ, Slattery H, Kilcoyne M, Hickey RM, Joshi L: Lectin microarray profiling and monosaccharide analysis of bovine milk immunoglobulin G oligosaccharides during the first 10 days of lactation. Food Sci Nutr 2019, 7(5):1564-1572.
  10. Valk-Weeber RL, Deelman-Driessen C, Dijkhuizen L, Eshuis-de Ruiter T, van Leeuwen SS: In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile. J Agric Food Chem 2020, 68(24):6544-6553.
  11. Gazi I, Reiding KR, Groeneveld A, Bastiaans J, Huppertz T, Heck AJR: Key changes in bovine milk immunoglobulin G during lactation: NeuAc sialylation is a hallmark of colostrum immunoglobulin G N-glycosylation. Glycobiology 2023, 33(2):115-125.
  12. Haakenson JK, Huang R, Smider VV. Diversity in the Cow Ultralong CDR H3 Antibody Repertoire. Front Immunol 2018, 9:1262.
  13. Mayer B, Kis Z, Frenyó LV, Hammarström L, Kacskovics I: The neonatal Fc receptor (FcRn) is expressed in the bovine lung. Vet Immunol Immunop 2004, 98:85-89.
  14. Weaver DM, Tyler JW, VanMetre DC, Hostetler DE and Barrington GM: Passive transfer of colostral immunoglobulins in calves. J Vet Intern Med 2000, 14:569–577.
  15. Chigerwe M, Tyler JW, Nagy DW, Middleton JR: Frequency of detectable serum IgG concentrations in precolostral calves. Am J Vet Res 2008, 69(6):791-795.
  16. Reber AJ, Donovan DC, Gabbard J, Galland K, Aceves-Avila M, Holbert KA, Marshall L, Hurley DJ: Transfer of maternal colostral leukocytes promotes development of the neonatal immune system I. Effects on monocyte lineage cells. Vet Immunol Immunopathol 2008, 123(3-4):186-196.
  17. Reber AJ, Donovan DC, Gabbard J, Galland K, Aceves-Avila M, Holbert KA, Marshall L, Hurley DJ: Transfer of maternal colostral leukocytes promotes development of the neonatal immune system Part II. Effects on neonatal lymphocytes. Vet Immunol Immunopathol 2008; 123(3-4):305-313.
  18. Rajala P, Castrén H: Serum immunoglobulin concentrations and health of dairy calves in two management systems from birth to 12 weeks of age. J Dairy Sci 1995, 78(12):2737-2744.
  19. Fratrić N, Ilić V, Milošević-Jovčić N, Stojić V: Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of PEG precipitable immune complexes in preruminant calves’ sera. Acta veterinaria 2010, 60(2-3):155-164.
  20. Murphy JM, Hagey JV, Chigerwe M: Comparison of serum immunoglobulin G half-life in dairy calves fed colostrum, colostrum replacer or administered with intravenous bovine plasma. Vet Immunol Immunopathol 2014, 158(3-4):233-237.
  21. Jennewein MF, Alter G: The Immunoregulatory Roles of Antibody Glycosylation. Trends Immunol 2017, 38(5):358-372.
  22. Alter G, Ottenhoff THM, Joosten SA : Antibody glycosylation in inflammation, disease and vaccination. Semin Immunol 2018, 39:102-110.
  23. Irvine EB, Alter G: Understanding the role of antibody glycosylation through the lens of severe viral and bacterial diseases. Glycobiology 2020, 30(4):241-253.
  24. Li D, Lou Y, Zhang Y, Liu S, Li J, Tao J: Sialylated immunoglobulin G: a promising diagnostic and therapeutic strategy for autoimmune diseases. Theranostics 2021, 11(11):5430-5446.
  25. Zlatina K, Galuska SP: Immunoglobulin Glycosylation – An Unexploited Potential for Immunomodulatory Strategies in Farm Animals. Front Immunol 2021, 12:753294.
  26. Vattepu R, Sneed SL, Anthony RM: Sialylation as an Important Regulator of Antibody Function. Front Immunol 2022, 13:818736.
  27. Krištić J, Lauc G. The importance of IgG glycosylation-What did we learn after analyzing over 100,000 individuals. Immunol Rev 2024, 328(1):143-170.
  28. Isernhagen L, Galuska CE, Vernunft A, Galuska SP. Structural Characterization and Abundance of Sialylated Milk Oligosaccharides in Holstein Cows during Early Lactation. Foods, 2024, 13(16):2484.
  29. Fratrić N, Gvozdić D, Vuković D, Savić O, Buač M, Ilić V: Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes’ IgG. Vet Immunol Immunopathol 2012, 150(3-4):161-168.
  30. Chase CC, Hurley DJ, Reber AJ: Neonatal immune development in the calf and its impact on vaccine response. Vet Clin North Am Food Anim Pract 2008, 24:87-104.
  31. Adler Y, Lamour A, Jamin C, Menez JF, Le Corre R, Shoenfeld Y, Youinou P: Impaired binding capacity of asialyl and agalactosyl IgG to Fc gamma receptors. Clin Exp Rheumatol 1995, 13:315–319.
  32. Kaneko Y, Nimmerjahn F, Ravetch JV: Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation. Science 2006, 313:670–673.
  33. Scallon BJ, Tam SH, McCarthy SG, Cai AN, Raju TS: Higher levels of sialylated Fc glycans in immunoglobulin G molecules can adversely impact functionality. Mol Immunol 2007, 44:1524–1534.
  34. Kovačić M, Fratrić N, Arsić A, Mojsilović S, Drvenica I, Marković D, Maslovarić I, Grujić Milanović J, Ivanov M, Ilić V: Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. Res Vet Sci 2020, 133:63-74.
  35. Tsuji S, Kato H, Matsuoka Y, Fukushima T: Molecular weight heterogeneity of bovine serum transferrin. Biochem Genet 1984, 22(11-12):1145-1159.
  36. Akerström B, Brodin TH, Reis K, Björck L: Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies. J Immunol 1985, 135:2589-2592.
  37. Zlatina K, Isernhagen L, Galuska CE, Murani E, Galuska SP. Changes in the N-glycosylation of porcine immunoglobulin G during postnatal development. Front Immunol 2024, 15:1361240.
  38. Corbeil LB: Antibodies as effectors. Vet Immunol Immunopathol 2002, 87, 169-175.
  39. Kovačić M, Marković D, Maslovarić I, Obrenović S, Grujić-Milanović J, Arsić A, Milanović Z, Savić O, Fratrić N, Ilić V: Serum proteins and lipids in mild form of calf bronchopneumonia: Candidates for reliable biomarkers. Acta Veterinaria 2017, 67(2):201-221.
  40. Altvater-Hughes TE, Hodgins HP, Hodgins DC, Bauman CA, Paibomesai MA, Mallard BA. Investigating the IgM and IgG B cell receptor repertoires and expression of ultralong complementarity determining region 3 in colostrum and blood from Holstein-Friesian cows at calving. Animals (Basel). 2024, 14(19):2841.
  41. Zhao Q, Li P, Wang B, Li B, Gao M, Ren G, Rile G, Rila S, Ma K, Bao F. Bovine ultra-long CDR H3 specific for bovine rotavirus displays potent virus neutralization and therapeutic effects in infected calves. Biomolecules 2025, 15(5):689.
  42. Duncan JR, Wilkie BN, Hiestand F, Winter AJ: The serum and secretory immunoglobulins of cattle: characterization and quantitation. J Immunol 1972, 108: 965-976.
  43. Cao J, Guo S, Arai K, Lo EH, Ning M: Studying extracellular signaling utilizing a glycoproteomic approach: lectin blot surveys, a first and important step. Methods Mol Biol 2013, 1013:227-233.
  44. Geyer H, Geyer R. Strategies for analysis of glycoprotein glycosylation. Biochim Biophys Acta 2006, 1764(12):1853-1869.
  45. Shinozuka Y, Suzuki N, KItsukawa M, Hayashi M, Suenaga N, Shimizu Y, Kurumisawa T, Kawai K. Longitudinal and cross-sectional studies to evaluate changes in cow milk microbiota over the lactation stages. Acta Veterinaria-Beograd 2024, 74(2):236-245.
  46. Shade KC, Conroy ME, Washburn N, Kitaoka M, Huynh DJ, Laprise E, Patil SU, Shreffler WG, Anthony RM: Sialylation of immunoglobulin E is a determinant of allergic pathogenicity. Nature 2020, 582(7811):265-270.
  47. Day CJ, Paton AW, Higgins MA, Shewell LK, Jen FE, Schulz BL, Herdman BP, Paton JC, Jennings MP: Structure aided design of a Neu5Gc specific lectin. Sci Rep 2017, 7(1):1495.
  48. Kovačić MV. Structural and functional properties of immune complexes in calves with bronchopneumonia [In Serbian: Структурне и функционалне особине имунокомплекса код телади оболеле од бронхопнеумоније]. Doctoral dissertation, University of Belgrade, Faculty of Chemistry, 2018.
DOI: https://doi.org/10.2478/acve-2025-0036 | Journal eISSN: 1820-7448 | Journal ISSN: 0567-8315
Language: English
Page range: 467 - 481
Submitted on: May 25, 2025
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Accepted on: Nov 6, 2025
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Published on: Dec 11, 2025
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Related subjects:

© 2025 Dejana D. Kosanović, Biljana Ž. Ristić, Marijana V. Kovačić, Radoš Ž. Knežević, Natalija P. Fratrić, Vesna Lj. Ilić, published by University of Belgrade, Faculty of Veterinary Medicine
This work is licensed under the Creative Commons Attribution 4.0 License.