Have a personal or library account? Click to login

Development of ELISA Milk-Based Test for Reference Sera Preparation and for Serological Detection of Bovine Brucellosis

Open Access
|Sep 2025

References

  1. WOAH: Brucellosis diagnostic tests 2022, (https://www.woah.org/en/what-we-do/animal-health-and-welfare/disease-data-collection/Accessed 19th June 2024).
  2. Morgan W J: The diagnosis of Brucella abortus infection in Britain. In: RP Crawford & RJ Hidalgo (Eds.), Bovine brucellosis, an international symposium. 1977, pp. 21–39. College Station: Texas A&M University Press.
  3. Nielsen K: Diagnosis of brucellosis by serology. Vet Microbiol 2002, 90: 447–459.
  4. Trotta A, Marinaro M, Cirilli M, Sposato A, Adone R, Beverelli M, Buonavoglia D, Corrente M: Brucella melitensis B115-based ELISA to unravel false positive serologic reactions in bovine brucellosis: a field study. BMC Vet Res 2020, 16(1):50.
  5. Godfroid J, Nielsen K, Saegerman C: Diagnosis of brucellosis in livestock and wildlife. Croat Med J 2010, 51, 296–305.
  6. Rahman AKMA, Smit S, Devleesschauwer B, Kostoulas P, Abatih E, Saegerman C, Shamsuddin M, Berkvens D, Dhand NK and Ward MP: Bayesian evaluation of three serological tests for the diagnosis of bovine brucellosis in Bangladesh. Epidemiol Infect 2019, 147: e73.
  7. Gall D, Nielsen K: Serological diagnosis of bovine brucellosis: a review of test performance and cost comparison. Rev Sci Tech 2004, 23(3):989-1002.
  8. Ducrotoy MJ, Conde-Alvarez R, Blasco JM, Moriyon I: A review of the basis of the immunological diagnosis of ruminant brucellosis. Vet Immunol Immunopathol 2016, 171:81–102.
  9. Praud A, Duran-Ferrer M, Fretin D, Jay M, O’Connor M, Stournara A, Tittarelli M, Travassos Dias I, Garin-Bastuji B: Evaluation of three competitive ELISAs and a fluorescence polarisation assay for the diagnosis of bovine brucellosis. Vet J 2016, 216:38-44.
  10. WOAH: Brucellosis: Brucella abortus. 2018, (https://www.cfsph.iastate.edu/diseaseinfo/factsheets/Accessed 19th June 2024).
  11. Andrade RS, de Oliveira MM, de Sousa Bueno Filho JS, Ferreira F, Godfroid J, Lage AP, Dorneles EMS: Accuracy of serological tests for bovine brucellosis: A systematic review and meta-analysis. Prev Vet Med 2023, 222, 106079.
  12. Van AA, Brioens P, Dekeyser P, Uytterrhagen L, Sijens RJ, Boey A: A comparative study of ELISA and other methods for the detection of Brucella antibodies in bovine sera. Vet Microbiol 1984, 10, 10–21.
  13. Nielsen K, Kelly L, Mallory M: Standardization of smooth lipopolysaccharide preparations for use in diagnostic serological tests for bovine antibody Brucella abortus. J Immunoassay 1998, 19(4):239-50.
  14. Noriello S: Laboratory-acquired brucellosis. Emerg Infect Dis. 2004, 10: 1848-1850.
  15. Terzi G, Büyüktanır Ö, Genç O, Gücükoğlu A, Yurdusev N: Detection of Brucella Antibody and DNA in Cow Milk by ELISA and PCR Methods. Kafkas Univ Vet Fak Derg 2010, 16 (Suppl-A): 47-52.
  16. Khan TI, Ehtisham-ul-Haque S, Waheed U, Khan I, Younus M and Ali S: Milk Indirect-ELISA and Milk Ring Test for Screening of Brucellosis in Buffaloes, Goats and Bulk Tank Milk Samples Collected from Two Districts of Punjab, Pakistan. Pak Vet J 2018, 38(1): 105-108.
  17. Nawaz M, Khan I, Shakeell M, Yousaf A, Naseer Z, Munibullah, Zohaib A, Hussain R and Ullah Q: Bovine and Caprine Brucellosis Detected by Milk Indirect ELISA and Milk Ring Test in Islamabad Capital Territory, Pakistan. Pakistan J Zool 2021,1-4.
  18. Caroff M, Bundle DR, Perry MB, Cherwonogrodzky JW, Duncan JR: Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3. Infect and Immun 1984, 46 (2): 384-388.
  19. Genç O, Büyüktanır Ö, Yurdusev N: Development of qualitative and quantitative ELISA models for bovine Brucellosis diagnosis. Kafkas Univ Vet Fak Derg 2010, 16: 287-291.
  20. Gen ç E, Serdar G, Kılıçoğlu Y, Genç O: Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. Kafkas Univ Vet Fak Derg 2022, 28(4): 431-436.
  21. DeLong ER, DeLong DM, Clarke-Pearson DL: Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics 1988, 44(3):837-845.
  22. McGiven JA: New developments in the immunodiagnosis of brucellosis in livestock and wildlife. Rev Sci Tech 2013, 32:163-176.
  23. Moreno E: Retrospective and prospective perspectives on zoonotic brucellosis. Front Microbiol 2014, 5: 213.
  24. Ducrotoy MJ, Munoz PM, Conde-Alvarez R, Blasco JM, Moriyon I: A systematic review of current immunological tests for the diagnosis of cattle brucellosis. Prev Vet Med 2018, 151:57–72.
  25. Vanzini VR, Aguirre NP, Valentini BS, Torioni de Echaide S, Lugaresi CI, Marchesino MD, Nielsen K: Comparison of an indirect ELISA with the Brucella milk ring test for detection of antibodies to Brucella abortus in bulk milk samples. Vet Microbiol 2001, 82(1):55-60.
  26. Nielsen K, Gall D, Jolley M, Leishman G, Balsevicius S, Smith P, Nicoletti P, Thomas F: A homogeneous fluorescence polarization assay for detection of antibody to Brucella abortus. J Immunol Methods 1996, 195(1-2):161-8.
  27. Arif S, Heller J, Hernandez-Jover M, McGill DM, Thomson PC: Evaluation of three serological tests for diagnosis of bovine brucellosis in smallholder farms in Pakistan by estimating sensitivity and specificity using Bayesian latent class analysis. Prev Vet Med 2018, 149, 21–28.
  28. Romero CS, Samama CM, Vincent JL, Wiberg S, Spahn DR: Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle. J Clin Microbiol 1995, 33(12): 3198–3200.
  29. Andrade RS, de Oliveira MM, de Sousa BFJS, Ferreira F, Godfroid J, Lage AP, Dorneles EMS: Accuracy of serological tests for bovine brucellosis: A systematic review and meta-analysis. Prev Vet Med 2024, Jan:222:106079. doi: 10.1016/j.prevetmed.2023.106079.
  30. Abdoel T, Dias IT, Cardoso R, Smits HL: Simple and rapid field tests for brucellosis in livestock. Vet Microbiol 2008, 130 (3–4): 312-319.
  31. Gusi AM, Bertu WJ, de Miguel MJ, Dieste-Perez L, Smits HL, Ocholi RA; Blasco JM, Moriyon I, Munoz PM: Comparative performance of lateral flow immunochromatography, iELISA and rose bengal tests for the diagnosis of cattle, sheep, goat and swine brucellosis. PLoS Negl Trop Dis 2019, 13, e0007509.
  32. Sotnikov DV, Berlina AN, Zherdev AV, Eskendirova SZ, Mukanov KK, Ramankulov YM, Mukantayev KN and Dzantiev BB: Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots. Appl Sci 2020, 10, 738:1-7.
  33. Genç O, Büyüktanır Ö, Yurdusev N: Rapid immunofiltration assay based on colloidal gold-protein G conjugate as an alternative screening test for bovine and ovine brucellosis: Trop Anim Health Prod 2012, 44(2):213-5.
DOI: https://doi.org/10.2478/acve-2025-0028 | Journal eISSN: 1820-7448 | Journal ISSN: 0567-8315
Language: English
Page range: 375 - 386
Submitted on: Oct 31, 2024
Accepted on: May 30, 2025
Published on: Sep 18, 2025
Published by: University of Belgrade, Faculty of Veterinary Medicine
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Related subjects:

© 2025 Evrim Genç, Özlem Büyüktanir Yaş, Oktay Genç, published by University of Belgrade, Faculty of Veterinary Medicine
This work is licensed under the Creative Commons Attribution 4.0 License.