Involvement of serotonergic, noradrenergic and gabaergic systems in the antinociceptive effect of a ketamine-magnesium sulfate combination in acute pain

Savić Vujović Katarina; Vučković Sonja; Stojanović Radan; Divac Nevena; Medić Branislava; Srebro Dragana; Kadija Marko; Prostran Milica

doi:10.2478/acve-2018-0009

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Involvement of serotonergic, noradrenergic and gabaergic systems in the antinociceptive effect of a ketamine-magnesium sulfate combination in acute pain

Acta Veterinaria

Volume 68 (2018): Issue 1 (March 2018)

By: Savić Vujović Katarina, Vučković Sonja, Stojanović Radan, Divac Nevena, Medić Branislava, Srebro Dragana, Kadija Marko and Prostran Milica

Open Access

|Mar 2018

Abstract

Ketamine and magnesium can interact in additive, supra-additive and antagonistic manners in analgesia or anesthesia. Ketamine is a non-competitive NMDA receptor antagonist. Magnesium is an endogenous non-competitive NMDA antagonist that causes anion channel blockade in a dose-dependent manner. It has been established that ketamine and magnesium interact synergistically in the tail-immersion test in rats.

To determine the role of serotonergic, GABAergic and noradrenergic systems in analgesia induced by the ketamine-magnesium sulfate combination.

Experiments were performed on male Wistar albino rats (200-250 g). Antinociception was evaluated by the tail-immersion test.

Methysergide (0.5 and 1 mg/kg, sc) administered alone did not affect nociception in rats. Methysergide (0.5 and 1 mg/kg, sc) antagonized the antinociceptive effect of the ketamine (5 mg/kg)-magnesium sulfate (5mg/kg) combination. Bicuculline (0.5 and 1 mg/kg, sc) given alone did not change the threshold to thermal stimuli in rats. Bicuculline (0.5 and 1 mg/kg, sc) antagonized the antinociceptive effect of the ketamine (5 mg/kg)-magnesium sulfate (5 mg/kg) combination. Yohimbine (0.5, 1 and 3 mg/kg, sc) applied alone did not change nociception. Yohimbine at a dose of 0.5 mg/kg did not influence the effect of ketamine (5 mg/kg)-magnesium sulfate (5 mg/kg), while yohimbine at doses of 1 and 3 mg/kg antagonized the antinociceptive effect of this combination.

Serotonergic, noradrenergic and GABAergic systems participate, at least in part, in the antinociceptive effect of the ketamine-magnesium sulfate combination in acute pain in rats.

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Articles in this issue

DOI: https://doi.org/10.2478/acve-2018-0009 | Journal eISSN: 1820-7448 | Journal ISSN: 0567-8315

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Language: English

Page range: 108 - 118

Submitted on: Jun 16, 2017

Accepted on: Dec 28, 2017

Published on: Mar 27, 2018

Published by: University of Belgrade, Faculty of Veterinary Medicine

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

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© 2018 Savić Vujović Katarina, Vučković Sonja, Stojanović Radan, Divac Nevena, Medić Branislava, Srebro Dragana, Kadija Marko, Prostran Milica, published by University of Belgrade, Faculty of Veterinary Medicine
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Volume 68 (2018): Issue 1 (March 2018)