Analytical approach for conversion of conventional to green RP-HPLC methods for analysis of lipophilic neutral and basic active pharmaceutical ingredients
References
- N. Nakov, J. Acevska, K. Brezovska, Z. Kavrakovski and A. Dimitrovska, Green Strategies Toward Eco-Friendly HPLC Methods in Pharma Analysis, in High Performance Liquid Chromatography – Recent Advances and Applications, IntechOpen, London 2023; https://doi.org/10.5772/intechopen.110035
- M. Yabré, L. Ferey, I. T. Somé and K. Gaudin, Greening reversed-phase liquid chromatography methods using alternative solvents for pharmaceutical analysis, Molecules 23(5) (2018) Article ID 1065 (25 pages); https://doi.org/10.3390/molecules23051065
- J. Namieśnik, Green analytical chemistry – some remarks, J. Sep. Sci. 24(2) (2001) 151–153; https://doi.org/10.1002/1615-9314(20010201)24:2<151::AID-JSSC151>3.0.CO;2-4
- P. T. Anastas and N. Eghbali, Green chemistry: Principles and practice, Chem. Soc. Rev. 39 (2010) 301–312; https://doi.org/10.1039/B918763B
- American Chemical Society, 12 Principles of Green Chemistry; https://www.acs.org/content/acs/en/greenchemistry/principles/12-principles-of-green-chemistry.html; last access date May 6, 2026.
- K. Gaudin, Potential of green solvents as mobile phases in liquid chromatography, J. Chromatogr. A 1750 (2025) Article ID 465810; https://doi.org/10.1016/j.chroma.2025.465810
- S. Bocian and S. Studzińska, Solvents for green pharmaceutical liquid chromatography – possibilities and limitations, J. Pharm. Biomed. Anal. 269 (2026) Article ID 117238; https://doi.org/10.1016/j.jpba.2025.117238
- O. Kalisz, M. Tobiszewski, A. Nowaczyk and S. Bocian, Exploring the potential of green chemistry in reversed-phase liquid chromatography: A review of sustainable solvents, Trends Anal. Chem. 181 (2024) Article ID 118007; https://doi.org/10.1016/j.trac.2024.118007
- J. Płotka, M. Tobiszewski, A. M. Sulej, M. Kupska, T. Górecki and J. Namieśnik, Green chromatography, J. Chromatogr. A 1307 (2013) 1–20; https://doi.org/10.1016/j.chroma.2013.07.099
- C. J. Welch, N. Wu, M. Biba, R. Hartman, T. Brkovic, X. Gong, R. Helmy, W. Schafer, J. Cuff, Z. Pirzada and L. Zhou, Greening analytical chromatography, Trends Anal. Chem. 29(7) (2010) 667–680; https://doi.org/10.1016/j.trac.2010.03.008
- S. N. Atapattu, Applications and retention properties of alternative organic mobile phase modifiers in reversed-phase liquid chromatography, J. Chromatogr. Open 5 (2024) Article ID 100135 (13 pages); https://doi.org/10.1016/j.jcoa.2024.100135
- P. M. Nowak, A. Bis and A. Zima, ChlorTox Base – A useful source of information on popular reagents in terms of chemical hazards and greenness assessment, Green Anal. Chem. 6 (2023) Article ID 100065 (6 pages); https://doi.org/10.1016/j.greeac.2023.100065
- M. Tobiszewski and J. Namieśnik, Scoring of solvents used in analytical laboratories by their toxicological and exposure hazards, Ecotoxicol. Environ. Saf. 120 (2015) 169–173; https://doi.org/10.1016/j.ecoenv.2015.05.043
- L. R. Snyder, J. J. Kirkland and J. L. Glajch, Practical HPLC Method Development, 2nd ed., Wiley, New York 1997.
- B. V. Trifunovska, A. Atanasova, P. Antovska, J. Lazova, K. Brezovska, A. Dimitrovska and N. Nakov, Transformation model to green HPLC methods for lipophilic acidic compounds based on isoeluotropic series of elution solvents, Microchem. J. 213 (2025) Article ID 113853; https://doi.org/10.1016/j.microc.2025.113853
- L. R. Snyder and H. Poppe, Mechanism of solute retention in liquid-solid chromatography and the role of the mobile phase in affecting separation: Competition versus “sorption”, J. Chromatogr. A, 184(4) (1980) 363–413; https://doi.org/10.1016/S0021-9673(00)93872-X
- E. Soczewiński, Mechanistic molecular model of liquid-solid chromatography: Retention-eluent composition relationships, J. Chromatogr. A 965(1–2) (2002) 109–116; https://doi.org/10.1016/S0021-9673(01)01278-X
- V. G. Asmita, S. G. Ashwini, V. B. Hupparage, M. Shubhrajit, K. Aditya and K. Jaydeep, Method development and validation of dapagliflozin by RP-HPLC, J. Pharm. Negat. Results 13(S6) (2022) 4316–4335; https://doi.org/10.47750/pnr.2022.13.S06.571
- T. Deepan and M. D. Dhanaraju, Stability-indicating HPLC method for the simultaneous determination of dapagliflozin and saxagliptin in bulk and tablet dosage form, Curr. Issues Pharm. Med. Sci. 31(1) (2018) 39–43; https://doi.org/10.1515/cipms-2018-0009
- United States Pharmacopeia – National Formulary (USP 48–NF 43), United States Pharmacopeial Convention, Rockville MD, USA; last access date May 06, 2026
- M. Maheshwari and P. Soni, RP-HPLC method development and validation for rapid estimation of diazepam in bulk and pharmaceutical dosage form, Res. J. Pharm. Technol. 15(5) (2022) 1938–1942; https://doi.org/10.52711/0974-360X.2022.00322
- DrugBank; https://www.drugbank.com; last access date May 06, 2026.
- G. Ramis-Ramos and M. C. García-Álvarez-Coque, Solvent Selection in Liquid Chromatography, in Liquid Chromatography, Elsevier, Amsterdam 2013, pp. 225–249; https://doi.org/10.1016/B978-0-12-415807-8.00010-9
- J. Frost, Regression Analysis: An Intuitive Guide for Using and Interpreting Linear Models, Jim Publishing, Central Missouri, USA, 2019.
- L. R. Snyder, Classification of the solvent properties of common liquids, J. Chromatogr. A 92(2) (1974) 223–230; https://doi.org/10.1016/S0021-9673(00)85732-5
- S. Bolton and C. Bon, Pharmaceutical Statistics: Practical and Clinical Applications, 5th ed., CRC Press, Boca Raton 2010; https://doi.org/10.3109/9781420074239
- International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use, ICH Harmonised Guideline, Validation of Analytical Procedures: Q2(R2), final version, November 2023; https://database.ich.org/sites/default/files/ICH_Q2%28R2%29_Guideline_2023_1130_ErrorCorrection_2025.pdf; last access date May 06, 2026
- A. Gałuszka, Z. M. Migaszewski, P. Konieczka and J. Namieśnik, Analytical eco-scale for assessing the greenness of analytical procedures, Trends Anal. Chem. 37 (2012) 61–72; https://doi.org/10.1016/j.trac.2012.03.013
- F. Pena-Pereira, W. Wojnowski and M. Tobiszewski, AGREE – Analytical GREEnness metric approach and software, Anal. Chem. 92(14) (2020) 10076–10082; https://doi.org/10.1021/acs.anal-chem.0c01887
Language: English
Accepted on: Aug 24, 2026
Published on: Sep 23, 2026
Published by: Croatian Pharmaceutical Society
In partnership with: Paradigm Publishing Services
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© 2026 Bojana Vulovska Trifunovska, Ana Atanasova, Packa Antovska, Katerina Brezovska, Aneta Dimitrovska, Natalija Nakov, published by Croatian Pharmaceutical Society
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.