Haemostatic activity of butanolic extracts of Lamium album and Lamium purpureum aerial parts

Bubueanu, Corina; Iuksel, Rasit; Panteli, Minerva

doi:10.2478/acph-2019-0026

Abstract

Lamium album and Lamium purpureum are species belonging to the genus Lamium. Aerial parts of the two species and roots of Lamium album have applications in human and veterinary traditional medicine. Haemostatic properties of butanolic extracts of Lamium species were investigated by two experimental models in Wistar rats: haemostatic test by tail bleeding time determination and acenocoumarolcarrageenan test. Results of the haemostatic test by tail bleeding determination demonstrated haemostatic activity of both extracts. In the acenocoumarol-carrageenan test, only the Lamium album extract showed haemostatic activity, comparable to that of vitamin K. Based on the qualitative chemical data on iridoid glycosides (HPTLC), 8-acetylshanzhiside methyl ester might be assumed to be responsible for haemostatic activity. Based on the acute toxicity test, none of the extracts showed toxicity.

References

1. N. Ito, R. Kakuda, Y. Yaoita and M. Kikuchi, Five new phenylethanoid glycosides from the whole plants of Lamium purpureum L., Chem. Pharm. Bull. 54 (2006) 1705–1708; https://doi.org/10.1248/cpb.54.170510.1248/cpb.54.1705
Search in Google Scholar Back to article
2 F. N. Yalçın and D. Kaya, Ethnobotany, pharmacology and phytochemistry of the genus Lamium (Lamiaceae), FABAD J. Pharm. Sci. 31 (2006) 43–52.
Search in Google Scholar Back to article
3 Z. P. Yordanova, M. K. Zhiponova, E. T. Iakimova, M. A. Dimitrova and V. M. Kapchina-Toteva, Revealing the reviving secret of the white dead nettle (Lamium album L.), Phytochem. Rev. 13 (2014) 375–389; https://doi.org/10.1007/s11101-014-9356-2410.1007/s11101-014-9356-2
Search in Google Scholar Back to article
4. R. Paduch, G. Matysik, M. Wójciak-Kosior, M. Kandefer-Szerszeń, A. Skalska-Kamińska, M. Nowak--Kryska and P. Niedziela, Lamium album extracts express free radical scavenging and cytotoxic activities, Polish J. Environ. Stud. 17 (2008) 569–580.
Search in Google Scholar Back to article
5. S. Damtoft, Iridoid glucosides from Lamium album, Phytochemistry31 (1992) 175–178; https://doi.org/10.1016/0031-9422(91)83030-O10.1016/0031-9422(91)83030-O
Search in Google Scholar Back to article
6. V. Hodisan, T. Perseca and E. Muica, Study on aminoacids from Lamium album L. (Lamiaceae family), Clujul Med. 52 (1979) 361–365.
Search in Google Scholar Back to article
7. G. Flamini, P. L. Cioni and I. Morelli, Composition of the essential oils and in vivo emission of volatiles of four Lamium species from Italy: L. purpureum, L. hybridum, L. bifidum and L. amplexicaule, Food Chem. 91 (2005) 63–68; https://doi.org/10.1016/J.FOODCHEM.2004.05.04710.1016/j.foodchem.2004.05.047
Search in Google Scholar Back to article
8. A. Et Sattar, N. Handgieva and S. Popov, Volatile constituents from Lamium maculatum leaves and Nepeta mushrum roots, Dokl. Bulg. Akad. Nauk46 (1993) 37–39.
Search in Google Scholar Back to article
9. C. Bubueanu, G. Campeanu, L. Pirvu and G. Bubueanu, Antioxidant activity of butanolic extracts of Romanian native species – Lamium album and Lamium purpureum, Rom. Biotechnol. Lett.18 (2013) 8855–8862.
Search in Google Scholar Back to article
10. H. Wagner and S. Bladt, Plant Drug Analysis. A Thin Layer Chromatography Atlas, 2nd ed, Springer-Verlag, Berlin-Heidelberg 1996, pp. 88–90.10.1007/978-3-642-00574-9
Search in Google Scholar Back to article
11. K. Greene, A. Schiviz, W. Hoellriegl, M. Poncz and E.-M. Muchitsch, Towards a standardization of the murine tail bleeding model, J. Thromb. Haemostasis8 (2010) 2820–2822; https://doi.org/10.1111/j.1538-7836.2010.04084.x10.1111/j.1538-7836.2010.04084.x21138523
Search in Google Scholar Back to article
12. M. Gábor and Z. Rázga, The acenocoumarol-carrageenin test, Acta Physiol. Hung.75(1990) 337–342.
Search in Google Scholar Back to article
13. R. Aiyalu, K. Muthuswamy and A. Ganesan, Haemostatic effect of fresh juice and methanolic extract of Eupatorium ayapana leaves in rat model, Int. J. Biol. Med. Res. 1 (2010) 85–87.
Search in Google Scholar Back to article
14. M. Li, R. Zhang, Z. Jia, J. Sheng, J. Qiu and J. Wang, Isolation and identification of hemostatic ingredients from Lamiophlomis rotata (Benth.) Kudo, Phytother. Res. 23 (2009) 816–822; https://doi.org/10.1002/ptr.266910.1002/ptr.266919173219
Search in Google Scholar Back to article
15. P. C. Fan, H. P. Ma, Y. Hao, X. R. He, A. J. Sun, W. Jiang, M. X. Li, L. L. Jing, L. He, J. Ma and Z. P. Jia, A new anti-fibrinolytic hemostatic compound 8-O-acetyl shanzhiside methylester extracted from Lamiophlomis rotata, J. Ethnopharmacol. 187 (2016) 232–238; https://doi.org/10.1016/j.jep.2016.04.01610.1016/j.jep.2016.04.01627085939
Search in Google Scholar Back to article

Haemostatic activity of butanolic extracts of Lamium album and Lamium purpureum aerial parts

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