Role of nitric oxide synthase on brain GABA transaminase activity and GABA levels

Lourdes A. Vega Rasgado; Guillermo Ceballos Reyes; Fernando Vega Díaz

doi:10.2478/acph-2018-0022

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Role of nitric oxide synthase on brain GABA transaminase activity and GABA levels

Acta Pharmaceutica

Volume 68 (2018): Issue 3 (September 2018)

By: Lourdes A. Vega Rasgado, Guillermo Ceballos Reyes and Fernando Vega Díaz

Open Access

|Jul 2018

Abstract

In an attempt to clarify the controversial role of nitric oxide (NO) in seizures, the effects of NO on brain GABA transaminase (GABA-T) activity and GABA levels were investigated. To this aim, the effects of the substrate (l-arginine) and inhibitors (Nω-nitro-l-arginine methyl ester, 7-nitroindazole) of NO synthase (NOS) on GABA-T activity and GABA levels in vitro and ex vivo were analyzed. In vitro NO diminished GABA-T activity and increased GABA. Ex vivo NO modified GABA-T activity and GABA levels biphasically. Inhibition of endothelial and neuronal NOS (eNOS and nNOS) had opposite effects on GABA-T activity and GABA levels, even during seizures induced by pentylenetetrazole. Different effects of NO on GABA-T activity and on GABA levels, depending on the NOS isoform involved, may explain its contradictory role in seizures, the endothelial NOS acting as an anticonvulsant and the neuronal NOS as a proconvulsant. nNOS inhibitors may represent a new generation of antiepileptics.

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Articles in this issue

DOI: https://doi.org/10.2478/acph-2018-0022 | Journal eISSN: 1846-9558 | Journal ISSN: 1330-0075

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Language: English

Page range: 349 - 359

Accepted on: Feb 12, 2018

Published on: Jul 4, 2018

Published by: Croatian Pharmaceutical Society

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

GABA,

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© 2018 Lourdes A. Vega Rasgado, Guillermo Ceballos Reyes, Fernando Vega Díaz, published by Croatian Pharmaceutical Society
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 68 (2018): Issue 3 (September 2018)