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TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound Cover

TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound

Acta Pharmaceutica
Volume 64 (2014): Issue 4 (December 2014)
By:  and    
Open Access
|Dec 2014
References

References

  1. 1. L. Liberti, D. Brown, T. Flaster, C. Lessing, J. Sutherland and M. Tims, Comfrey, in The Review of Natural Products (Eds. A. DerMarderosian and J. A. Beutler), Wolters Kluwer Health, St. Luis 2006.
    Search in Google Scholar
  2. 2. E. Teuscher, G. Willuhn and D. Loew, Symphyti Radix, in Teedrogen und Phytopharmaka (Ed. M. Wichtl), 5th ed., Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart 2009, pp. 644-646.
    Search in Google Scholar
  3. 3. J. Bruneton, Pyrrolizidine Alkaloids, in Pharmacognosy - Phytochemistry Medicinal Plants, 2nd ed., Intercept Ltd., Andover 1999, pp. 833-848.
    Search in Google Scholar
  4. 4. E. Röder and V. Neuberger, Pyrrolizidinalkaloide in Symphytum-Arten, Dtsch. Apoth. Ztg. 128 (1988) 1991-1994.
    Search in Google Scholar
  5. 5. C. E. Couet, J. Hopley and A. B. Hanley, Metabolic activation of pyrrolizidine alkaloids by human, rat and avocado microsomes, Toxicon 34 (1996) 1058-1061; DOI: 10.1016/0041-0101(96)00056-6.
    Open DOISearch in Google Scholar
  6. 6. Y. P. Wang, J. Yan, P. P. Fu and M. W. Chou, Human liver microsomal reduction of pyrrolizidine alkaloid N-oxides to form the corresponding carcinogenic parent alkaloid, Toxicol. Lett. 155 (2005) 411-420; DOI: 10.1016/j.toxlet.2004.11.010.
    Open DOISearch in Google Scholar
  7. 7. R. Chizzola, Rapid sample preparation technique for the determination of pyrrolizidine alkaloids in plant extracts, J. Chromatogr. A 668 (1994) 427-433; DOI: 10.1016/0021-9673(94)80135-5.
    Open DOISearch in Google Scholar
  8. 8. U. Zweifel and J. Luethy, Collaborative experiments on the determination of pyrrolizidine alkaloids (PAs) in Symphytum extracts, Pharm. Arch. Helv. 65 (1990) 165-169.
    Search in Google Scholar
  9. 9. H. Wagner, U. Neidhardt and G. Tittel, DC- und HPLC-Analyse der Pyrrolizidin-N-oxid-Alkaloide von Symphyti Radix, Planta Med. 41 (1981) 232-239; DOI: 10.1055/s-2007-971708.
    Open DOISearch in Google Scholar
  10. 10. C. E. Couet, C. Crews and A. B. Hanley, Analysis, separation, and bioassay of pyrrolizidine alkaloids from comfrey (Symphytum officinale), Nat. Toxins 4 (1996) 163-167; DOI: 10.1002/19960404NT3.
    Open DOISearch in Google Scholar
  11. 11. J. C. Altamirano, S. R. Gratz and K. A. Wolnik, Investigation of pyrrolizidine alkaloids and their N-oxides in commercial comfrey-containing products and botanical materials by liquid chromatography electrospray ionization mass spectrometry, J. AOAC Int. 88 (2005) 406-412.
    Open DOISearch in Google Scholar
  12. 12. H. J. Huizing, F. de Boer and M. Malingré, Preparative ion-pair high-performance liquid chromatography and gas chromatography of pyrrolizidine alkaloids from comfrey, J. Chromatogr. A 214 (1981) 257-262; DOI: 10.1016/S0021-9673(00)98534-0.
    Open DOISearch in Google Scholar
  13. 13. T. Mroczek, K. Glowniak and A. Wlaszczyk, Simultaneous determination of N-oxides and free bases of pyrrolizidine alkaloids by cation-exchange solid-phase extraction and ion-pair highperformance liquid chromatography, J. Chromatogr. A 949 (2002) 249-262; DOI: 10.1016/S0021-9673(01)01498-4.
    Open DOISearch in Google Scholar
  14. 14. G. Tittel, H. Hinz and H. Wagner, Quantitative Bestimmung der Pyrrolizidinalkaloide in Symphyti radix durch HPLC, Planta Med. 37 (1979) 1-8; DOI: 10.1055/s-0028-1097287.
    Open DOISearch in Google Scholar
  15. 15. J. C. Wuilloud, S. R. Gratze, B. M. Gamble and K. A. Wolnik, Simultaneous analysis of hepatotoxic pyrrolizidine alkaloids and N-oxides in comfrey root by LC-ion trap mass spectrometry, Analyst 129 (2004) 150-156; DOI: 10.1039/B311030C.
    Open DOISearch in Google Scholar
  16. 16. J. M. Betz, R. M. Eppley, W. C. Taylor and D. Andrzejewski, Determination of pyrrolizidine alkaloids in commercial comfrey products (Symphytum sp.), J. Pharm. Sci. 83 (1994) 649-653; DOI: 10.1002/jps.2600830511.
    Open DOISearch in Google Scholar
  17. 17. N. H. Oberlies, N.-C. Kim, D. R. Brine, B. J. Collins, R. W. Handy, C. M. Sparacino, M. C. Wani and M. E. Wall, Analysis of herbal teas made from the leaves of comfrey (Symphytum officinale): reduction of N-oxides results in order of magnitude increases in the measurable concentration of pyrrolizidine alkaloids, Public Health Nutr. 7 (2004) 919-924; DOI: 10.1079/PHN2004624.
    Open DOISearch in Google Scholar
  18. 18. E. Röder, H. Wiedenfeld, R. Kersten and R. Kröger, Determination of open chain pyrrolizidine alkaloids by capillary gas chromatography, Planta Med. 56 (1990) 522; DOI: 10.1055/s-2006-961075.
    Open DOISearch in Google Scholar
  19. 19. G. Aurnhammer, B. Dengler, L. Hörhammer, P. H. List, B. Mühlenbruch, M. Rattenberger, H. Roth, H. J. Roth, R. Schaette, W. Schmid and K.-H. Surborg, Symphytum, in Hagers Handbuch der pharmazeutischen Praxis, Sechster Band, Chemikalien und Drogen, Teil B: R, S (Eds. P. H. Lisz and L. Hörhammer), 4th ed. Springer Verlag, Berlin 1978, pp. 706-710.
    Search in Google Scholar
  20. 20. D. Janeš, B. Kalamar and S. Kreft, Improved method for isolation of lycopsamine from roots of comfrey (Symphytum officinale), Nat. Prod. Commun. 7 (2012) 861-862.
    Search in Google Scholar
  21. 21. E. Roeder, Analysis of Pyrrolizidine Alkaloids, Curr. Org. Chem. 3 (1999) 557-576.
    Open DOISearch in Google Scholar
  22. 22. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2(R1), Current Step 4 version, November 2005; http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf; access date December 8, 2013.
    Search in Google Scholar
DOI: https://doi.org/10.2478/acph-2014-0031 | Journal eISSN: 1846-9558 | Journal ISSN: 1330-0075
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Language: English
Page range: 503 - 508
Accepted on: Jun 6, 2014
Published on: Dec 20, 2014
Published by: Croatian Pharmaceutical Society
In partnership with: Paradigm Publishing Services
Keywords:
comfrey,
lycopsamine,
retrorsine,
TLC,
densitometry
Related subjects:
Pharmacy,
Pharmacy, other

© 2014 Damjan Janeš, Samo Kreft, published by Croatian Pharmaceutical Society
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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