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Effects of early dexamethasone treatment on several markers of inflammation and fibrosis in an animal model of lung silicosis in rats – A pilot study Cover

Effects of early dexamethasone treatment on several markers of inflammation and fibrosis in an animal model of lung silicosis in rats – A pilot study

Open Access
|Dec 2022

Figures & Tables

Fig 1

Differential count of leukocytes in the blood (%) in the control groups with saline instead of silica (Sal), in the silica-injected and non-treated animals (Sil), and in the silica-injected and dexamethasone-treated animals (Dex) after 14 or 28 days of the treatment delivery. Abbreviations: Ly: lymphocytes, Neu: neutrophils, Mo: monocytes, Eo: eosinophils. Statistical differences among the groups: a p<0.05 for Sil14 vs Sal14; b p<0.05 for Sil28 vs Sil14; c p<0.05 for Dex14 vs Sil14; d p<0.05 for Dex28 vs Sil28.

Fig 2

Differential count of leukocytes in the BAL fluid (%) in the control groups with saline instead of silica (Sal), in the silica-injected and non-treated animals (Sil), and in the silica-injected and dexamethasone-treated animals (Dex) after 14 or 28 days of the the treatment delivery. Abbreviations: Ma: macrophages, Neu: neutrophils, Eo: eosinophils, Ly: lymphocytes. Statistical differences among the groups: a p<0.05 and b p<0.001 for Sil14 vs Sal14; d p<0.05 and e p<0.001 for Sil28 vs Sal28; f p<0.05 for Sil28 vs Sil14; g p<0.05 and h p<0.01 for Dex28 vs Sil28.

Fig 3

Thickness of the wall of bronchioles and pulmonary vessels (in μm) – staining for presence of collagen by Sirius red in the control groups with saline instead of silica (Sal), in the silica-injected and non-treated animals (Sil), and in the silica-injected and dexamethasone-treated animals (Dex) after 14 or 28 days of the treatment delivery. Statistical differences among the groups: a p<0.05 for Sal28 vs Sal14, b p<0.001 for Sil14 vs Sal14, c p<0.01 for Sil14 vs Sal14, d p<0.001 for Sil28 vs Sal28, e p<0.01 for Sil28 vs Sal28, f p<0.01 for Dex14 vs Sil14, g p<0.001 for Dex28 vs Sil28.

Fig 4

Thickness of the wall of bronchioles and pulmonary vessels (in μm) – staining for presence of smooth muscle actin (SMA) by antibody against SMA in the control groups with saline instead of silica (Sal), in the silica-injected and non-treated animals (Sil), and in the silica-injected and dexamethasone-treated animals (Dex) after 14 or 28 days of the treatment delivery. Statistical differences among the groups: a p<0.05 for Sil14 vs Sal14, b p<0.001 for Sil28 vs Sal28, c p<0.01 for Sil28 vs Sal28, d p<0.01 for Dex14 vs Sil14, e p<0.001 for Dex28 vs Sil28.

Absolute counts of leukocytes in the blood and in the BAL fluid in the control groups with saline instead of silica (Sal), in the silica-injected and non-treated animals (Sil), and in the silica-injected and dexamethasone-treated animals (Dex) after 14 or 28 days of the treatment delivery_

14 days28 days
Leukocytes in the blood (x 109/L)
Sal14Sil14Dex14Sal28Sil28Dex28
Ly2.95±1.074.33±0.84 a1.99±0.87 g3.21±0.834.18±1.231.42±0.53 j
Neu0.63±0.190.97±0.401.14±0.400.61±0.220.67±0.221.49±0.79 h
Mo0.04±0.020.03±0.010.04±0.020.05±0.030.05±0.020.03±0.01
Eo0.03±0.030.04±0.040.05±0.050.03±0.040.06±0.040.04±0.02
Leukocytes in the BAL fluid (x 103/mL)
Sal14Sil14Dex14Sal28Sil28Dex28
Ma97.7±41.595.4±37.7146±38.8 f107±45.9102±43.884.3±42.4
Neu2.29±1.6614.3±3.83 c35.1±49.93.51±1.3526.8±24.1 d8.68±5.23
Eo0.25±0.320.81±0.23 b1.47±1.570.23±0.232.39±2.17 d0.62±0.35 h
Ly0.99±0.642.35±1.704.06±2.100.71±0.274.92±2.89 e1.40±0.87 i
DOI: https://doi.org/10.2478/acm-2022-0012 | Journal eISSN: 1338-4139 | Journal ISSN: 1335-8421
Language: English
Page range: 102 - 114
Submitted on: Oct 18, 2022
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Accepted on: Nov 13, 2022
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Published on: Dec 22, 2022
Published by: Sciendo
In partnership with: Paradigm Publishing Services
Publication frequency: 3 issues per year

© 2022 Adamcakova Jana, Balentova Sona, Hanusrichterova Juliana, Barosova Romana, Mikolka Pavol, Prso Kristian, Mokry Juraj, Kalenska Dagmar, Kunertova Lenka, Mokra Daniela, published by Sciendo
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