Table 1
Indicators of experimental and control rats adenohypophysis after adaptation to long-term exposure to heavy metal salts complex (X ± SD).
| Indicator | Series of animals | |
|---|---|---|
| Control rats (n=6) | Experimental rats (n=6) | |
| Pituitary length, mm | 7.92±0.83 | 6.64±0.59 |
| Pituitary width, mm | 4.26±0.19 | 3.63±0.2* |
| Capsule thickness, μm | 3.52±0.27 | 1.42±0.19*** |
| Vessels area, μm2 | 215.83±5.96 | 224.3±7.96 |
| Chromophobic cells | 57.46 ± 2.58 | 65.15 ± 0.43 * |
| Chromophilic acidophiles | 31.28 ± 1.71 | 30.2 ± 0.59 |
| Chromophilic basophils | 11.26 ± 0.06 | 4.65 ± 0.18 *** |
| ACTH (pg / ml) | 591.0±1.83 | 319.5 ± 3.17*** |
Fig.1
Morphological rearrangements in the experimental rat pituitary gland under the condition of 30-days adaptation to heavy metals salts: 1 – venous pleural effusion; 2 – edema tissue trabeculae; 3-collagenization of the vessel walls. Staining: A – by hematoxylin-eosin; B – by Mason-Goldner.
Fig. 2
Morphological rearrangements in the experimental rats pituitary gland under the condition of 30-days adaptation to heavy metals salts: 1 – edema of connective tissue trabeculae; 2 – discomplexation of cellular trabeculae; 3 – cyst. Staining: by hematoxylin-eosin.
Fig. 3
Cellular composition of the experimental rats adenohypophysis after the condition 2 of 30-day readaptation to heavy metal salts: 1 – chromophilic basophils; 2 – chromophobes; 3 – chromophilic acidophiles. Staining: by Mason Goldner.
Table 2
Expression level of HSP90 in adenohypophysis adenocytes of control and experimental rats after adaptation to long-term exposure to heavy metal salts complex (X ± SD).
| HSP90 expression | Control group total number 6 animals | Experimental group total number 6 animals |
|---|---|---|
| Low positive 1 | 69.05±0.16 | – |
| Moderately positive 2 | 31.91±0.82 | 42.75±1.26*** |
| Strong positive 3 | – | 57.14±1.61*** |
Fig.4
Expression of hsp90 in the cytoplasm of experimental animals adenocytes under the con dition of 30-day readaptation to 90-day exposure to heavy metal salts: 1 – high level of hsp90 expression in the adenocytes cytoplasm. Immunohistochemical study of hsp90 expression.