Table 1
The sequences of primers used in the analysis
| GENE NAME | FORWARD PRIMER | REVERSE PRIMER |
|---|---|---|
| CD105 | CTCAGGTCCCCAATGCTACC | GGTTGAAGGCCAGGTAGAGT |
| CD73 | CCCATTGACGAACGGAACAA | TATACCACGTGAATTCCGCC |
| CD14 | CACTAGAGCCCTGCGAAGTA | CGACGGCAATCATACACTGG |
| CD34 | ATGAGACCTCCAGCTGTGAG | AGGTCAGACTGGTGCTTTCT |
| CD90 | CGAGAATGCTACCACCTTGC | AGCCGGAGTTCACATGTGTA |
| CD45 | ACCTAGGCAAACATGTGAGGA | CTTCCAGATCAAAATTTCCACGA |
| HPRT | CCATCACATCGTAGCCCTC | ACTTTTATATCGCCCGTTGAC |
| ACTB | CCCTTGCCGCTCCGCCTTC | GCAGCAATATCGGTCATCCAT |
Figure 1
Photographs of morphological changes between the beginning and end-point of in vitro culture. Taken using an inverted microscope, using a relief contrast and 10x magnification.
Figure 2
The results of the flow cytometry analysis of the available canine ASC markers. The coloured peaks represent the cells stained by the specific antibodies, while the transparent peaks correspond to isotype controls.
Figure 3
The results of the RT-qPCR analysis of the change in expression of ASC specific markers during a 14-day primary culture of adipocyte derived stem cells. All data was presented as a log10 of fold change.