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Persistence of dengue genome in a remotely infected patient Cover

Persistence of dengue genome in a remotely infected patient

Asian Biomedicine
Volume 17 (2023): Issue 6 (December 2023)
By: Soraya Thaivanich,  Jirayu Visuthranukul,  Kesinee Arunyingmongkol,  Udomsak Bunworasate,  Padet Siriyasatien and  Wanla Kulwichit  
Open Access
|Dec 2023

Figures & Tables

Figure 1.

Dengue RNA was detected in the cellular compartment of mobilized bone marrow-derived hematopoietic stem cells by RT-nested PCR targeting the dengue envelope region. The RT-nested PCR products were separated on 2% agarose gels along with the DNA marker indicated on the left side in base pairs (M). Lane 1 = mobilized hematopoietic stem cells, lane 2 = cell-free compartment (supernatant), lane 3 = cell pellet fraction, lane 4 = negative control. Lanes 1–4 are all from blood collected after G-CSF administration; dengue RNA was not detected in any fractions from pre-G-CSF blood (data not shown). Lanes 5–8 = dengue serotypes 1–4, respectively, as positive controls. G-CSF, granulocyte colony-stimulating factor; PCR, polymerase chain reaction; RT, reverse transcription.
Dengue RNA was detected in the cellular compartment of mobilized bone marrow-derived hematopoietic stem cells by RT-nested PCR targeting the dengue envelope region. The RT-nested PCR products were separated on 2% agarose gels along with the DNA marker indicated on the left side in base pairs (M). Lane 1 = mobilized hematopoietic stem cells, lane 2 = cell-free compartment (supernatant), lane 3 = cell pellet fraction, lane 4 = negative control. Lanes 1–4 are all from blood collected after G-CSF administration; dengue RNA was not detected in any fractions from pre-G-CSF blood (data not shown). Lanes 5–8 = dengue serotypes 1–4, respectively, as positive controls. G-CSF, granulocyte colony-stimulating factor; PCR, polymerase chain reaction; RT, reverse transcription.

Detection of dengue viral RNA in various fractions of blood obtained before and after G-CSF injection to mobilize hematopoietic stem cells into peripheral blood

Post-G-CSF blood fractionsPre-G-CSF blood fractions
CFMSCCPSRPBMCCP
Dengue envelope-specific RT-PCRNegPosNegNegNegNeg
DOI: https://doi.org/10.2478/abm-2023-0072 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
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Language: English
Page range: 287 - 290
Published on: Dec 28, 2023
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year
Keywords:
dengue,
G-CSF,
hematopoietic,
pathogenesis,
persistence,
serotype,
stem cell person with remote infection
Related subjects:
Medicine,
Assistive professions, nursing,
Basic medical science,
Basic medical science, other,
Clinical medicine,
Clinical medicine, other

© 2023 Soraya Thaivanich, Jirayu Visuthranukul, Kesinee Arunyingmongkol, Udomsak Bunworasate, Padet Siriyasatien, Wanla Kulwichit, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution 4.0 License.

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