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Antibody response to enterotoxigenic Bacteroides fragilis of Filipino colorectal cancer patients Cover

Antibody response to enterotoxigenic Bacteroides fragilis of Filipino colorectal cancer patients

Open Access
|Dec 2023

Figures & Tables

Figure 1.

An overview of the method adopted in this study. CRC, colorectal cancer; ELISA, enzyme-linked immunosorbent assay; ETBF, enterotoxigenic Bacteroides fragilis.
An overview of the method adopted in this study. CRC, colorectal cancer; ELISA, enzyme-linked immunosorbent assay; ETBF, enterotoxigenic Bacteroides fragilis.

Figure 2.

Checkerboard titration results at 1:1,000 secondary antibody dilution: X-axis = primary antibody (pooled plasma samples) concentration; Y-axis = anti-ETBF IgG antibody level absorbance at OD450 nm. (A) 80 μg/mL coating antigen; (B) 50 μg/mL coating antigen; (C) 25 μg/mL coating antigen; (D) 10 μg/mL coating antigen; (E) 5 μg/mL coating antigen. ETBF, enterotoxigenic Bacteroides fragilis.
Checkerboard titration results at 1:1,000 secondary antibody dilution: X-axis = primary antibody (pooled plasma samples) concentration; Y-axis = anti-ETBF IgG antibody level absorbance at OD450 nm. (A) 80 μg/mL coating antigen; (B) 50 μg/mL coating antigen; (C) 25 μg/mL coating antigen; (D) 10 μg/mL coating antigen; (E) 5 μg/mL coating antigen. ETBF, enterotoxigenic Bacteroides fragilis.

Figure 3.

Comparison of optical density (OD) values (nanometer) of anti-ETBF IgG and anti-ETBF IgA levels between CRC patients and healthy individuals (cases, n = 39; controls, n = 39). (A) Bacterial lysate as coating antigen; (B) culture broth as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.
Comparison of optical density (OD) values (nanometer) of anti-ETBF IgG and anti-ETBF IgA levels between CRC patients and healthy individuals (cases, n = 39; controls, n = 39). (A) Bacterial lysate as coating antigen; (B) culture broth as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.

Figure 4.

Comparison of the mean OD values (nanometer) of the anti-ETBF IgG and IgA antibody levels of CRC patients when grouped by (A) tumor grade and (B) tumor stage using bacterial lysate as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.
Comparison of the mean OD values (nanometer) of the anti-ETBF IgG and IgA antibody levels of CRC patients when grouped by (A) tumor grade and (B) tumor stage using bacterial lysate as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.

Figure 5.

Comparison of the mean OD values (nanometer) of the anti-ETBF IgG and IgA antibody levels of CRC patients when grouped by tumor grade (A) and tumor stage (B) using bacterial culture broth as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.
Comparison of the mean OD values (nanometer) of the anti-ETBF IgG and IgA antibody levels of CRC patients when grouped by tumor grade (A) and tumor stage (B) using bacterial culture broth as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.

Demographic and clinical characteristics of CRC cases and controls

CharacteristicsCases (n = 39)Controls (n = 39)P value*
Sex
  Male22 (56%)22 (56%)
  Female17 (44%)17 (44%)
Age at initial diagnosis (years)
  <5011 (28%)10 (26%)0.49
  ≥5028 (72%)29 (74%)0.43
Mean age at initial diagnosis (years ± SD)58 ± 12
Median age at initial diagnosis61
Tumor grade
  G1 (well differentiated)11 (28%)
  G2 & G3 (moderately and poorly differentiated)17 (44%)
  No information available11 (28%)
Tumor stage
  T1 & T2 (early)8 (20%)
  T3 & T4 (advanced)21 (54%)
  No information available10 (26%)
DOI: https://doi.org/10.2478/abm-2023-0070 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
Language: English
Page range: 273 - 280
Published on: Dec 28, 2023
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year

© 2023 Ana Maria D. Cariño, Gregg Austine Balanag, Edrienne Myenna Magat, Allan Fellizar, Teresa Sy Ortin, Oliver Villaflores, Leonardo Guevarra, Pia Marie Albano, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution 4.0 License.